Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,978,243	G→T	intergenic (‑46/+275)	flhD ← / ← insB5	DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,978,243	0	G	T	100.0%	100.1 / NA	29	intergenic (‑46/+275)	flhD/insB5	DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/14);  total (15/14)

ATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACA  >  NC_000913/1978015‑1978481                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               aTTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGaa                                                                                                                                                                                                                          <  2:14160/251‑1 (MQ=255)         cttacAGCTTAACCATTTGAGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGAAAGTAGTAAATATGACAAGTTGATGTCATAACTGTGTTTCAGCAACTCGGAGGTATGCATTCTTCCCACCCAGAATAACCAAATTTATTTTTATGCGGTTTCACATCACCACGCGATGTCGCCGGCAAGCCCCGGt                                                                                                                                                                                                                  >  2:199168/5‑251 (MQ=255)            ccAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTaaa                                                                                                                                                                                                               <  1:149153/251‑1 (MQ=255)                   aaCCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATAGGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGGCATGTCTCCGGTAAGCCCCGGTAAAAaataat                                                                                                                                                                                                        <  1:124358/251‑1 (MQ=255)                      cagttGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAg                                                                                                                                                                                                     <  2:177641/248‑1 (MQ=255)                        tttGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGc                                                                                                                                                                                                    >  2:70619/1‑250 (MQ=255)                                      aGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCTCCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGct                                                                                                                                                                                     <  2:135546/251‑2 (MQ=255)                                      aGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCg                                                                                                                                                                                     >  2:24452/1‑251 (MQ=255)                                              tAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACTCTGTGCAAGTAGTAAATATGCCCCGTTGCTTTAATAACTTTGTTTCAGCAACTCGGAGGTCTGCATTATTCCCACCCAGAATAACCAACTTTCTTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTg                                                                                                                                                                             >  1:193841/1‑251 (MQ=255)                                                  gTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAAt                                                                                                                                                                         <  1:152516/251‑1 (MQ=255)                                                    tGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATa                                                                                                                                                                        <  2:76537/250‑1 (MQ=255)                                                                              gaCGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAatat                                                                                                                                             >  2:287922/1‑251 (MQ=255)                                                                                       tagaggACGCTTTGTACTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTAc                                                                                                                                     >  2:110425/5‑250 (MQ=255)                                                                                                                  aaCGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCt                                                                                                         <  2:11548/251‑1 (MQ=255)                                                                                                                  aaCGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCAGTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTGTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCt                                                                                                         <  1:24452/251‑1 (MQ=255)                                                                                                                  aaCGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGAAGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCt                                                                                                         <  2:263798/251‑1 (MQ=255)                                                                                                                    cGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTa                                                                                                        >  2:308356/1‑250 (MQ=255)                                                                                                                              ggagacagtatgcgatgttcttgacATAACTGTGTCTCAACTACTCGGAGGAATGCATTATTACCACCCAGCATAACCAACTTTTTTTTTATGCGGTTTCACCTCACCCCGTGATCTCGCCGGGAATACCCGGAAACAAATAATTAGACTTAGAATAGTTCCGATCAGCTGCAATAACCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAACTGGGACTTAACACTTATCTAAGATTTTACCt                                                                                             >  2:220057/25‑251 (MQ=255)                                                                                                                                             aGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTg                                                                              >  2:186052/1‑251 (MQ=255)                                                                                                                                                         aaaTGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACt                                                                   >  1:102274/1‑250 (MQ=255)                                                                                                                                                                                gTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAgggggg                                           >  1:169222/1‑251 (MQ=255)                                                                                                                                                                                   tGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACACAGTGATGTCGCCGGGACGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAAACACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTg                                        >  1:178426/1‑251 (MQ=255)                                                                                                                                                                                     cATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGTGGCTGCg                                      <  2:222355/251‑1 (MQ=255)                                                                                                                                                                                                 ccaGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCaataat                          <  1:282106/251‑1 (MQ=255)                                                                                                                                                                                                           cAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGc                 >  2:204085/1‑250 (MQ=255)                                                                                                                                                                                                                        aTGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAac   >  2:173293/1‑251 (MQ=255)                                                                                                                                                                                                                          gCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAaca  <  1:70619/250‑1 (MQ=255)                                                                                                                                                                                                                                 cACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCa                               >  2:147374/1‑214 (MQ=255)                                                                                                                                                                                                                                 cACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCa                               <  1:147374/214‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               ATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACA  >  NC_000913/1978015‑1978481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑