Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,823,167	A→G	Y237C (TAT→TGT)	nadE →	NH3‑dependent NAD(+) synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,823,167	0	A	G	100.0%	90.0 / NA	28	Y237C (TAT→TGT)	nadE	NH3‑dependent NAD(+) synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/22);  total (6/22)

GGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGT  >  NC_000913/1822920‑1823396                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      ggTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCt                                                                                                                                                                                                                                    <  2:366046/251‑1 (MQ=255)          gtggGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACGGTCTGGAAGGGaa                                                                                                                                                                                                                           <  1:115005/251‑1 (MQ=255)           tggGCACCGATCATGCAGCAGCAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAACCAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGCGGCACTCGGCGTGACCTATGACAATATCGCCGACTGTCtaaaccgga                                                                                                                                                                                                                            >  1:156554/1‑241 (MQ=255)           tggGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGTAACACCTGTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCt                                                                                                                                                                                                                                    <  2:156554/241‑1 (MQ=255)             ggCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGGGACCTATGACAATATCGACGACTGTCTGGAAGGGaaaa                                                                                                                                                                                                                         >  1:196067/1‑250 (MQ=255)                 ccGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTa                                                                                                                                                                                                                    <  1:323230/251‑1 (MQ=255)                 ccGATAATGCCGCAGAAGCCAGTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGGATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAActta                                                                                                                                                                                                                    <  1:323232/251‑4 (MQ=255)                          cagcagAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAg                                                                                                                                                                                                           <  2:114187/251‑1 (MQ=255)                                    cATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGaa                                                                                                                                                                                                 <  1:103012/251‑1 (MQ=255)                                     aTTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCACCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTCTAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGCCGCCAGAAc                                                                                                                                                                                                >  1:153404/1‑251 (MQ=255)                                        aCCGGATTCGTCACTAAAGATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACaa                                                                                                                                                                                              <  2:209217/250‑1 (MQ=255)                                                                 aCGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGaa                                                                                                                                                                    <  2:123096/251‑1 (MQ=255)                                                                   gactgtgcGGACATTAATCCGCTGTATCGTCTCCACAAACGTCAGGGTAAACAGGTACTTGCTGCATTAGCTTCACCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTTGAAGATTATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCTTTACCTATGTCAATATCTACGACTGTCTGGAAGTGAAAAACGTACCTCAACAGTTCTGCAGAACAATAGTGAACTGGTATCTGAAAACCgctct                                                                                                                                                                  <  2:74200/244‑5 (MQ=255)                                                                                                       aaaCGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGAttt                                                                                                                              <  2:13833/251‑1 (MQ=255)                                                                                                                                  gcATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGTTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACatat                                                                                                   <  1:178603/251‑1 (MQ=255)                                                                                                                                  gcATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGGACatat                                                                                                   <  2:40048/251‑1 (MQ=255)                                                                                                                                  gcATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACatat                                                                                                   <  2:285581/251‑1 (MQ=255)                                                                                                                                      tAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTgg                                                                                                <  1:280702/250‑1 (MQ=255)                                                                                                                                      tAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACGCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTTAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTAGCTCATATTgg                                                                                                <  1:132090/250‑1 (MQ=255)                                                                                                                                      tAGCTTGCCCGGAACACCTTTATAAGAAAGCGCAAACGGACGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGGCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTGCTGGAAAAAGTAATAATTTGCACATATTgg                                                                                                <  2:301871/250‑1 (MQ=255)                                                                                                                                         cTTGCCAGGTACACCTTTATCAGAAAGCGACAACGGCAGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATtg                                                                                            >  2:163989/1‑251 (MQ=255)                                                                                                                                           tGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATtgt                                                                                           <  2:334422/250‑1 (MQ=255)                                                                                                                                              ccGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCg                                                                                        >  1:117619/1‑250 (MQ=255)                                                                                                                                                         ttATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTa                                                                              <  1:203915/249‑1 (MQ=255)                                                                                                                                                              aGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGAAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGtt                                                                       >  2:298017/1‑251 (MQ=255)                                                                                                                                                                                              cGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCg                                        <  2:339127/250‑1 (MQ=255)                                                                                                                                                                                               gCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTGTCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGGAATTGGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAc                                      <  1:220726/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccTATGACAATATCGACGACTGGCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGGATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAAGTTGCACATAGTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCAGCGTGAACGCCTTATTCGACCTAGAAAACTATTCAAATTCAATGCATTGCAGGAGt  <  2:39873/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      GGTGTCGTGGTGGGCACCGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGTCAGGGTAAACAGTTACTGGCGGCATTAGCTTGCCCGGAACACCTTTATAAGAAAGCGCCAACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGT  >  NC_000913/1822920‑1823396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑