Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,985	C→T	K5K (AAG→AAA)	rpmI ←	50S ribosomal subunit protein L35

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,985	0	C	T	100.0%	81.8 / NA	24	K5K (AAG→AAA)	rpmI	50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

ACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTCCATACCGATTTGCTGG  >  NC_000913/1799739‑1800228                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aCGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTa                                                                                                                                                                                                                                                  <  1:4238/250‑1 (MQ=255)          atatGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGc                                                                                                                                                                                                                                         >  1:91634/1‑250 (MQ=255)                      cTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACtt                                                                                                                                                                                                                            >  1:311338/1‑251 (MQ=255)                        gTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTc                                                                                                                                                                                                                           <  1:68668/250‑1 (MQ=255)                                  tCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCAtt                                                                                                                                                                                                                >  2:232734/1‑251 (MQ=255)                                    tAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGt                                                                                                                                                                                                              >  1:196472/1‑251 (MQ=255)                                          aaaaGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTGTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCCCCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTtaataa                                                                                                                                                                                                        <  2:224590/251‑1 (MQ=255)                                                   aaaCGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACaa                                                                                                                                                                                               <  1:110088/251‑1 (MQ=255)                                                      cGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAgg                                                                                                                                                                                            <  2:121847/251‑1 (MQ=255)                                                             ggCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACa                                                                                                                                                                                      >  2:353747/1‑250 (MQ=255)                                                                     cGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAACACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCtgtg                                                                                                                                                                             >  1:3202/1‑251 (MQ=255)                                                                                           cAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCacagac                                                                                                                                                        >  1:51904/1‑250 (MQ=255)                                                                                                ccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTGTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCCCCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCt                                                                                                                                                  <  2:84824/251‑1 (MQ=255)                                                                                                                            cGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTa                                                                                                                       <  1:37847/250‑1 (MQ=255)                                                                                                                                             tacgtttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTTGGAGCGAGCACCATGat                                                                                                      <  2:168650/250‑1 (MQ=255)                                                                                                                                                                    aGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGt                                                                              >  1:178044/1‑251 (MQ=255)                                                                                                                                                                                        gtgcttgtgcttAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTg                                                          <  2:29825/251‑1 (MQ=255)                                                                                                                                                                                                    aaaaCCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATGCGACCACTGCCATTTCTTGc                                               <  2:19567/250‑1 (MQ=255)                                                                                                                                                                                                                         tttGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTACTAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTa                          >  2:62612/1‑250 (MQ=255)                                                                                                                                                                                                                         tttGAAGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGAGATTa                          >  1:31765/1‑250 (MQ=255)                                                                                                                                                                                                                             aaGCGCTTAGCAGCACCGCGAACGGTTTTAATTTTTGGCCTTTTAATCACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGACGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCAc                     >  2:215129/1‑251 (MQ=255)                                                                                                                                                                                                                              aGCGCTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACt                    <  1:117483/251‑1 (MQ=255)                                                                                                                                                                                                                                  cTTAGCAGCACCGCGTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTcc                 >  2:334095/1‑250 (MQ=255)                                                                                                                                                                                                                                                gTACGGTTTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTCCATACCGATTTGCTgg  >  2:226306/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTCCATACCGATTTGCTGG  >  NC_000913/1799739‑1800228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑