Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,673,859	G→A	intergenic (‑150/‑54)	mdtU ← / → tqsA	protein MdtU/autoinducer 2 exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,673,859	0	G	A	100.0%	68.3 / NA	20	intergenic (‑150/‑54)	mdtU/tqsA	protein MdtU/autoinducer 2 exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/4);  total (16/4)

GCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCG  >  NC_000913/1673620‑1674085                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                   gcgcCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGaaa                                                                                                                                                                                                                         <  1:377549/251‑1 (MQ=255)             ctCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACattatt                                                                                                                                                                                                              >  2:344026/1‑250 (MQ=255)                          aaGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGgaag                                                                                                                                                                                                <  1:125765/251‑1 (MQ=255)                                                     gTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGc                                                                                                                                                                     >  2:145926/1‑251 (MQ=255)                                                                  aTAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGCACAAGCATGGCAAAGCCGATCATCACGCt                                                                                                                                                        >  2:275042/1‑251 (MQ=255)                                                                          cTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGcc                                                                                                                                                >  1:26188/1‑251 (MQ=255)                                                                                                     ttATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGc                                                                                                                     >  1:98524/1‑251 (MQ=255)                                                                                                        tCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTgg                                                                                                                  >  1:81725/1‑251 (MQ=255)                                                                                                                                   gatgatAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAGAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTg                                                                                       <  1:517/251‑1 (MQ=255)                                                                                                                                                       gTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTCTCACTTCTTTACCTGAGCGGATTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCg                                                                   >  1:48372/1‑251 (MQ=255)                                                                                                                                                         tGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGtt                                                                 >  2:160101/1‑251 (MQ=255)                                                                                                                                                                                   ttAAGAATTAACTCACAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGATCGTAAAATACATTATTATTAGCAAAGGAATAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGACCTAAAAATCGTCATTATGTTGGGAATGCTGGTCCTTATTCTCTGCGATATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCCTTATTTATTGCTGtt                                        >  2:248000/1‑250 (MQ=255)                                                                                                                                                                                     aaGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTAtt                                     >  1:108090/1‑251 (MQ=255)                                                                                                                                                                                         aTTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCtt                                  >  2:54101/1‑250 (MQ=255)                                                                                                                                                                                                      atTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGAGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGc                     >  2:366899/1‑250 (MQ=255)                                                                                                                                                                                                        tttCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAac                  >  1:68078/1‑251 (MQ=255)                                                                                                                                                                                                              ttcttTACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATgg            >  1:82286/1‑251 (MQ=255)                                                                                                                                                                                                                   tACCTGAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTTATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGc       >  2:262763/1‑251 (MQ=255)                                                                                                                                                                                                                        gAGCGGTTTGATTTTCGTTATGATAACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCGCGCATTATTTATGGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGc   <  1:218746/250‑1 (MQ=255)                                                                                                                                                                                                                        gAGCGGTTTGATTTTCGATATGATAACGGAGCGCACAAGACATTATTATTACCAAACGAAGCACAAACCGGGAAACGCATCCCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTCTTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCg  >  1:213342/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                   GCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAATTGTCCTCGGTAGTTGAACACGCCTGATTTGTATCATAGCTTAAGAATTAACTCAAAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGGAAGAAAAAACGGGGACAAGCATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCG  >  NC_000913/1673620‑1674085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑