Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	413,605	C→T	E717K (GAA→AAA)	sbcC ←	ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	413,605	0	C	T	100.0%	111.3 / NA	33	E717K (GAA→AAA)	sbcC	ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

AGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTCCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAAT  >  NC_000913/413358‑413839                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           agagTTTGCGCCTGCCGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGATCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCaa                                                                                                                                                                                                                                         <  2:102021/251‑1 (MQ=255) agagTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCaa                                                                                                                                                                                                                                         <  2:108133/251‑1 (MQ=255)   ggTTTGCGCCTGACGGGGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATa                                                                                                                                                                                                                                       <  2:186158/250‑1 (MQ=255)      ttGCGCCTGACGGCGCTGGTTTTCCATATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATAcc                                                                                                                                                                                                                                     <  1:31685/250‑1 (MQ=255)      ttGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATAcc                                                                                                                                                                                                                                     <  2:342019/250‑1 (MQ=255)              gACGGCGCTGGCTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCTGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACAGTGTGCATGTACCTGCCGCCAGTTTTTCAATACCACAGTttc                                                                                                                                                                                                                             <  1:168909/250‑1 (MQ=255)                  gcgcTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTCGTGATTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCCAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCGAGTTTTTCAATACCACAGTTTCTTCGc                                                                                                                                                                                                                        >  1:47094/1‑251 (MQ=255)                        gTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCg                                                                                                                                                                                                                  >  2:58002/1‑251 (MQ=255)                            tCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCggg                                                                                                                                                                                                                >  1:210115/1‑249 (MQ=255)                                          cagccgttccctgcgggTTAGTGTTTTTTCCTTCATTAGCTGCGCAAGGAACGCCTTCTGGTCCTCAAAGGCGCTTGCCTGTAGCGCGGTTGT‑AAACTGGGCCCTGGTTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCg                                                                                                                                                                                                <  1:231696/235‑1 (MQ=255)                                                    aGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTc                                                                                                                                                                                      >  2:371403/1‑251 (MQ=255)                                                        gCGTTAGTGATTGTTCATCCATTAGCGCCGCAAGGACCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTcc                                                                                                                                                                                     >  2:222962/1‑248 (MQ=255)                                                                   tgttCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCa                                                                                                                                                                        <  1:3323/250‑1 (MQ=255)                                                                                                      aTCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGt                                                                                                                                    >  2:380228/1‑251 (MQ=255)                                                                                                                                  gt‑gtCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGcttc                                                                                                        >  2:247919/1‑251 (MQ=255)                                                                                                                                                 cTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACCTTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGCGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGACTCCGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCg                                                                                          >  1:240785/1‑251 (MQ=255)                                                                                                                                                                  tttGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCtcttct                                                                         >  2:72893/1‑251 (MQ=255)                                                                                                                                                                             aGAACATCATGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCtg                                                              >  2:143808/1‑251 (MQ=255)                                                                                                                                                                                  aTCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGc                                                          >  1:154222/1‑250 (MQ=255)                                                                                                                                                                                       gTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGt                                                     <  1:58002/250‑1 (MQ=255)                                                                                                                                                                                       gTTGCTGGAACGTCTGCTGCTGGCTGTGTATTGCGAGACAGTGATCATGTACCTGACGCCAGTTTTTCAATACCACAGTTGCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTGTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCGGCTCTGCGCGTCGTGCTGACGTGTCGCCAACCAGCTCTCGTCTTCGTCTCCCTGTCGCAATGTc                                                    <  2:40145/251‑1 (MQ=255)                                                                                                                                                                                         tGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACATGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTc                                                    >  1:158415/1‑249 (MQ=255)                                                                                                                                                                                            tGTAACTTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCGCTTCTTCATCTTCCTGTGGCAATGTCAGTg                                                <  2:282023/250‑1 (MQ=255)                                                                                                                                                                                                       ctgctgGCTGTGTAATGCGAGACATTGATCATGTACCTGCCGCCAGGTTTTCAATACCACAGATTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCAGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGATTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTc                                     >  1:11517/1‑250 (MQ=255)                                                                                                                                                                                                             gcagtgtAATGCGAGACATTGTTAATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGCCGTCAGCTGCTGAATACGGTTTTGCAGCGAGGTTAATTCGTTCTGGCGTTGCTGCCACCTCTGCGCTTCTTGCTGCCGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCCATggcg                              >  2:81205/4‑248 (MQ=255)                                                                                                                                                                                                                gtgtAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTa                           <  2:87307/251‑1 (MQ=255)                                                                                                                                                                                                                gtgtAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTa                           <  1:259343/251‑1 (MQ=255)                                                                                                                                                                                                                     aTGCGAGACATTGTTCATGTACCTGCCGCAAGTTTTTCAATACCACAGTTTCTTCGCAGTGAGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCACTGTCAGTGCATAACCCGTCAATGTCGTTAAAagt                      >  2:378083/1‑251 (MQ=255)                                                                                                                                                                                                                       gCGAGACATTGTTCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCGCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAagta                     <  1:222962/250‑1 (MQ=255)                                                                                                                                                                                                                                   tCATGTACCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAGCCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCgtt         <  1:232085/250‑1 (MQ=255)                                                                                                                                                                                                                                         aCCTGCCGCCAGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAAt  >  2:164793/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTc     <  2:14146/238‑1 (MQ=255)                                                                                                                                                                                                                                                   aGTTTTTCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTc     >  1:14146/1‑238 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           AGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGT‑GTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACATTGTTCATGTACCTGCCGCCAGTTTTCCAATACCACAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAAT  >  NC_000913/413358‑413839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑