Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,576,911	C→A	G237C (GGC→TGC)	yddB ←	putative TonB‑dependent receptor YddB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,576,911	0	C	A	100.0%	70.4 / NA	24	G237C (GGC→TGC)	yddB	putative TonB‑dependent receptor YddB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/12);  total (12/12)

CCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTATAATCACGGCTGGAGCCGGTATATTTTAAGGTTAAATCGTGGGTAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCCGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACC  >  NC_000913/1576679‑1577152                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  caaaTTTCGCGATAAGAGAGAGGGAAGGGGCTGGTATTATAATCACGGCTGGAGCCGGTATATTTTAAGGTTAAATCGTGGGTAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGAGATATCAGACTGGCGGCGCGATAAACCGGCGGGAACGCAGAAGTTATCAGCGAGTTc                                                                                                                                                                                                                                 >  1:250983/4‑251 (MQ=255)                               cTGGTATTATAATCACGGCTGGAGCCGGTATATTTTAAGGTTAAATCGTGGGTAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGAATAAAAGTTCtt                                                                                                                                                                                                   >  1:280344/1‑251 (MQ=255)                                      tataATCACGGCTGGAGCCGGTATATTTTAAGGTTAAATCGTGGGTAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTaaa                                                                                                                                                                                             >  2:79811/1‑250 (MQ=255)                                         aaTCACGGCTGGAGCCGGTATATTTTAAGGTTAAATCGTGGGTAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCt                                                                                                                                                                                         <  2:192822/251‑1 (MQ=255)                                                                tttAAGGTTAAATAGTGGGTACAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGGAAAGAAGAAGTTATCAGAAAGTTCCTGCTTAAACGACAAGGGATAAAAGTTTTTTTTACAATCTGGGGCGTAACAAGTAcctcatg                                                                                                                                                                   >  2:372539/1‑244 (MQ=255)                                                                                   tAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATgcg                                                                                                                                               >  1:309394/1‑251 (MQ=255)                                                                                            cGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGtt                                                                                                                                       <  2:238053/250‑1 (MQ=255)                                                                                                  cAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtctc                                                                                                                                 >  2:154156/1‑250 (MQ=255)                                                                                                            aaaTTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCg                                                                                                                      >  1:32084/1‑251 (MQ=255)                                                                                                                      aaTGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAAcc                                                                                                             <  1:57182/250‑1 (MQ=255)                                                                                                                        tGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATACACCGGCGGCAACGCAGAAGGTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAATAATTCCTGAAGAGCCTTGATTAAATTCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGt                                                                                                          >  2:290695/1‑251 (MQ=255)                                                                                                                            ggaTCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTTGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCATTCCGa                                                                                                      <  2:242097/249‑1 (MQ=255)                                                                                                                            gTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGa                                                                                                      <  2:242099/251‑1 (MQ=255)                                                                                                                             tATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGa                                                                                                      >  1:63479/1‑250 (MQ=255)                                                                                                                                               aCTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAAccc                                                                                   >  2:235962/1‑251 (MQ=255)                                                                                                                                                          ccGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCtt                                                                        <  2:250983/251‑1 (MQ=255)                                                                                                                                                                aCAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATTCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTatc                                                                  <  2:143733/251‑1 (MQ=255)                                                                                                                                                                           agtCGAAACATAATCCGCGCGGGTGATAGCAGACTGGCGTCGCGATAAGCCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACTACAAGGTATAAAAGTACTTTTTAAAAGCTGGTGAGTAATAAGTACTTCCTGAAGAGCCTTGATGAAATGCGCTCTTGTTATTCTCATCTATATGCGATGTTAACCAGTCCGAACGCTTATTGCGATAACCCAATTTCACCGTGCTATCATCGGCGTTgt                                                       <  2:52030/249‑2 (MQ=255)                                                                                                                                                                                aaaCATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGGTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAAGTTCACCTTGCTATCATCAGCGTTGAAGCGt                                                  <  1:170126/251‑1 (MQ=255)                                                                                                                                                                                  aCATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGttt                                                <  2:178853/251‑1 (MQ=255)                                                                                                                                                                                        cccGCGCGGGTGATAGCAGACTGGCGGCGCGATAAACCGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTGCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCTCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAAATTCACCGTTCTATCATCAGCGTTGAAGCGTTTGATCtt                                          <  1:343778/250‑1 (MQ=255)                                                                                                                                                                                                                      gATAAAACGGCGGTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGAcc            >  1:304648/1‑251 (MQ=255)                                                                                                                                                                                                                              ggcggTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCa     >  2:247122/1‑250 (MQ=255)                                                                                                                                                                                                                                cggTAACGCAGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGTTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATTTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCCCCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCAGCAATTCCCCCGCCATTGAAGCGACCAAATTCAAcc  <  1:110584/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  CCCATTTCGCGATCAGACTGCGGGAAGGTGCTGGTATTATAATCACGGCTGGAGCCGGTATATTTTAAGGTTAAATCGTGGGTAAAGCGGTCGCTGGCAAACCAGGTAAATTTGCTTAATGCAGTATCGATAACGTTTTTATACTGTGCCCGACCGGCGACAATGCCGTCATTCGAAACATAATCCGCGCGGGTGATATCAGACTGGCGGCGCGATAAACCGGCGGTAACGCCGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACC  >  NC_000913/1576679‑1577152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑