Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,699	0	A	C	59.0%	‑6.7 / 45.0	39	S63A (TCA→GCA)	tfaQ	putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base A (10/6);  new base C (11/12);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATT  >  NC_000913/1634454‑1634886                                                                                                                                                                                                                                                      |                                                                                                                                                                                             aaTATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCtcat                                                                                                                                                                                         <  1:377911/251‑1 (MQ=11)   gatGCTCGCTGGCTACCTGCATCAGGCTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGGTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAAAGAGATGCCACGATGCCtcatca                                                                                                                                                                                       <  2:225059/250‑1 (MQ=11)      gCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTg                                                                                                                                                                                    <  1:136754/251‑1 (MQ=11)                 aCCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAg                                                                                                                                                                         <  1:376778/250‑1 (MQ=31)                  ccTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACACCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGcc                                                                                                                                                                       >  2:64250/1‑251 (MQ=11)                        tCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATGCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGAAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGACGCCGGAAGCCACGTGATAAAACGTTTTACCCCGATGATCTTCTACGAGATGCCACGCTGACTCATCACGGTTGA‑AAACAGCCACGa                                                                                                                                                                   >  2:276555/1‑249 (MQ=11)                               gttTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAGCGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAagccagcc                                                                                                                                                           <  2:255161/250‑1 (MQ=14)                                tttgttGTTTCCTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCTAGTTCAGAAATAAAGAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCGCGAGGACTCGGTACTTTTGA‑AAACATCCATGAAGCCAGCCg                                                                                                                                                          <  2:151796/246‑1 (MQ=11)                                 tttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCgg                                                                                                                                                         >  1:93356/1‑250 (MQ=11)                                                ccTCCCGGATCCGGAACAGTTTTTCTGCTCCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCa                                                                                                                                         <  1:185702/251‑1 (MQ=11)                                                       gATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCgg                                                                                                                                  <  1:3470/251‑1 (MQ=31)                                                        aTCCGGCACAGATTTTCTGCTTCAGTGTCCTTCACCCAGGATGTGCCGTTCCTCTTCTGAAACTCCCCTTCCGGCGATACCCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGCAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACTATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGt                                                                                                                                 >  2:233292/1‑251 (MQ=11)                                                              aaccgTTTTTCTTCTTCCTTATCGTCCACCCGGGCTGTGCCTTTCCACTTCTGATATTCCCCCCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGAACCGAGTTCAAAATTAAATACCCCGTCGCCGAAAGCCACGTCATAGAC‑GGTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGttt                                                                                                                          <  2:365508/247‑1 (MQ=11)                                                                cAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTg                                                                                                                         <  1:205293/251‑1 (MQ=11)                                                                                  aTCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATg                                                                                                         >  2:108261/1‑249 (MQ=11)                                                                                              ggCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATg                                                                                           >  2:73111/1‑251 (MQ=11)                                                                                                 tgtgCCGTTCCACTTCTCAAACTCCCCTTCCGGCGATAACCCGGAAACATTTTCCGGTAACGGACCGAGTTCAGAAATCAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCAGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGTTGCAATATCGGTCCTGCTTGCTGGCAGACCTGTATGAGGCGGAATATATGCAt                                                                                        >  1:351066/1‑251 (MQ=11)                                                                                                          ccACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCa                                                                                <  2:71250/250‑1 (MQ=31)                                                                                                               tctgatattCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGTCGGAATATATTCGTCGCCTTCACCAAt                                                                           <  1:82552/242‑1 (MQ=11)                                                                                                               tCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAATCCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAAt                                                                           >  1:373253/1‑250 (MQ=14)                                                                                                                    aaCTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGCAATAAATAACGCGTCGACGGAAGCCACGTCCTAAACCGTTTTAACCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGCGCAATATCGGTACTGTTTGCTGGCAGAACTGTATTCGGCGGAATATATGCCTCACCTTCACCAATAAATTc                                                                     >  2:146645/1‑251 (MQ=11)                                                                                                                       tacccTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAATCCACGTCATAGACGGTTTTACACCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTGTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCAt                                                                   <  2:76618/248‑1 (MQ=11)                                                                                                                        ccccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCGCTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGATGCGGAATAGATGCATCACCTGCACCAATAAATTCAt                                                                   <  2:233735/249‑1 (MQ=9)                                                                                                                          cctcccGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGt                                                               >  2:201437/5‑251 (MQ=11)                                                                                                                          cctcccGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGt                                                               >  2:70946/5‑251 (MQ=11)                                                                                                                           ctcccGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGGATGAGGGGGAATATATGCGTCACCTGCACCAATAAATTCATTAGtt                                                              >  2:294115/4‑251 (MQ=11)                                                                                                                                ggCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGc                                                         >  2:64722/1‑251 (MQ=31)                                                                                                                                 gCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGTGACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGcc                                                        <  2:240020/251‑1 (MQ=11)                                                                                                                                          aGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGAtt                                                >  2:196474/1‑250 (MQ=31)                                                                                                                                           ggTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATtat                                              <  2:117849/251‑1 (MQ=11)                                                                                                                                                aaTTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTCTGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAAtt                                         <  2:364233/250‑1 (MQ=11)                                                                                                                                                        ggTAACGGACCGAGTTCAGAAATAAATAACGAGTCGCCGGAAGCAACGACATAGACGGTATTACCCCGATGGTCTTCCACGAGCTGCCCAGCAGCCTCATCACTGTTGACAAAC‑GCCACAAAGCCCGCAGGAAAATCTGGCGGTGCAATATAGGTACTGTTTGCAGGCAGACCGGTCTGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGt                                 >  1:221240/1‑251 (MQ=2)                                                                                                                                                          tAACGGAACGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCCTTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTcc                               >  2:217669/1‑251 (MQ=11)                                                                                                                                                             cGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAAACAGTTTTACCCCGATGATCTTCAACGAGATGCAACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTCCTGTTTGCTGGAAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCCTTAGTTCCAGCCAGCAGATTATAAATTTTTATGGTCCGTg                            >  1:59684/1‑251 (MQ=11)                                                                                                                                                                    aGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTc                     <  2:320875/251‑1 (MQ=11)                                                                                                                                                                          gAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCAt               >  1:22930/1‑251 (MQ=11)                                                                                                                                                                           aaataaatAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGA‑AAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCAtt              >  1:79928/1‑251 (MQ=11)                                                                                                                                                                            aataaataACGCGTCGCCGGAAGCCACGTCATACACCGTTTTACACCGATGATCTTCAACGAGATGCCACGATAACTCATCCCTGTTGA‑AAACAGCCACGAAGCAAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTc             >  1:210167/1‑251 (MQ=9)                                                                                                                                                                                        gTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTATTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCAtt  >  2:256949/1‑250 (MQ=14)                                                                                                                                                                                                                                                      |                                                                                                                                                                                             AATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGA‑AAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATT  >  NC_000913/1634454‑1634886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑