Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,303,073	Δ12 bp	coding (175‑186/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1303072	1 (0.030)	23 (0.630)	22/490	0.8	95.8%	coding (174/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	1303085 =	1 (0.030)					coding (187/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

CAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGC  >  NC_000913/1302829‑1303302                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       cAGGAGTGTCCTGCTCCACGTTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGGGATGGCCAATATCGAAGCGAAATATCATCTGAATGATCCGtatttca                                                                                                                                                                                                                                 <  2:267370‑M1/251‑8 (MQ=255)               tCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaaca                                                                                                                                                                                                                    >  2:98066‑M1/1‑230 (MQ=255)                                          aCAGAAGGCACAGGACAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGACGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcg                                                                                                                                                                                        >  1:302644‑M1/1‑203 (MQ=255)                                              aaGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtcc                                                                                                                                                                                            >  2:290406‑M1/1‑199 (MQ=255)                                              aaGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtcc                                                                                                                                                                                            <  1:290406‑M1/243‑45 (MQ=255)                                               aGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaa                                                                                                                                                                                   <  2:99601‑M1/251‑54 (MQ=255)                                                ggCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacgttgattgcggtccatcgtttaaa                                                                                                                                                                                  <  2:354983‑M1/251‑55 (MQ=255)                                                                                        aCGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagtt                                                                                                                                          <  2:143271‑M1/251‑95 (MQ=255)                                                                                                  cTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttc                                                                                                                                >  2:103962‑M1/1‑147 (MQ=255)                                                                                                                          attCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcat                                                                                                         <  2:108412‑M1/250‑128 (MQ=255)                                                                                                                                   aCTATTTAGTTCTTTTTGTTGCGCCTAGCGACGGGCAGCCCTTTTACCGGTGAACGTACTTTGCCGGCAGAAGTGATGGACAATATCGAAGCGAAATATCATCTTAATGATCC‑‑‑‑‑‑‑‑‑‑‑‑GTATTTCAGCTCCCTGAAACAACTGGCGCCCGGTGTTTTCGGTCAATCGTTTAAATATACAGATTATTCGGTGAATGACTTGGGTGCATCACGTTTTCTCGTTTCTGCCAAACTGGGAGCCGCAGGATTTTTCTTTGc                                                                                   >  2:336995/1‑251 (MQ=255)                                                                                                                                      aTTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggt                                                                                            >  1:262425‑M1/1‑111 (MQ=255)                                                                                                                                                   tgatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgtt                                                                               >  1:229395‑M1/1‑98 (MQ=255)                                                                                                                                                    gatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgtt                                                                               <  1:250235‑M1/250‑154 (MQ=255)                                                                                                                                                                gcCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgtt                                                                   >  1:302809‑M1/1‑85 (MQ=255)                                                                                                                                                                      aaGCCATTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccg                                                            <  2:195015‑M1/251‑173 (MQ=255)                                                                                                                                                                          ccatctaacggcaaccgtactttaccgctagTAGTGATGGCCAATATCGAAGCGAAATCTCATCCTAACGATCCGtatttcagctacccgcaacaaatggcgcacggtgatttcggtccatcgtttaactataaagattattcggtcaatgtcttggttgcatccagttttcccgtttctgcaacactgggcgccgcagcatttttccttgcggtaatactgggtgttagtcctggcgttattgccgcatt                                                        >  2:237385‑M1/16‑75 (MQ=255)                                                                                                                                                                                                                  ccAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctg                <  2:275169‑M1/251‑217 (MQ=255)                                                                                                                                                                                                                     atatCGAAGCGAACTATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctggc              >  1:129421‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                      tatCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctggca             >  1:276645‑M1/1‑31 (MQ=255)                                                                                                                                                                                                                           aaGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctggcaatgacc       <  1:158719‑M1/251‑226 (MQ=255)                                                                                                                                                                                                                            aGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctggcaatgaccg      >  2:18160‑M1/1‑25 (MQ=255)                                                                                                                                                                                                                              cGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctggcaatgaccg      >  2:252547‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                                aaaTATCATCTTAATGATCCGtagttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagagtattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaggggactataccgtgatgggtctgtcaatgttcggggt  <  2:54397‑M1/251‑231 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       CAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGC  >  NC_000913/1302829‑1303302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.