Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	120.9 / NA	35	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/23);  total (12/23)

CATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAC  >  NC_000913/3485968‑3486449                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      cATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAGTCACCAg                                                                                                                                                                                                                                          <  2:39563/251‑1 (MQ=255)     gATGTACTGCATGTATGCAAAGTACGTCACATTACCGTGCAGTACAGTTGATCGCCCTTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCGGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTTCCCATCCAAGAGCAAGCTTATTCACCAGGGTg                                                                                                                                                                                                                                      <  2:352294/251‑1 (MQ=255)      aTGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGTGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTTCTTGGCAAACCGCAAACAGACTCGACTCTCGAATGGTTCTTGTCTCATTGTCACATTCATAAGTACCCATCCAAGAGCAAGCTTAGTCTCCATGGTGa                                                                                                                                                                                                                                     <  2:209392/251‑1 (MQ=255)             gCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAg                                                                                                                                                                                                                                <  2:382158/249‑1 (MQ=255)             gCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑Cg                                                                                                                                                                                                                             <  1:176291/251‑1 (MQ=255)              cATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑Cgg                                                                                                                                                                                                                            >  1:127284/1‑251 (MQ=255)                 atATGCAAAGGACTTCACATTACCGTGCAGTACATTTGATAGCCCGTCCCCATGGATCGGGAACGATTTTTCGGAAATCCAGAGACAGCGGCGTTTGCTGGCTCTGGAGAAACCTTAAAACAGATGATAACCTGGCATGGTGCTTGGGAAACCGCAAACAGTCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCAGAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGGGAAAAAG‑CGGaaa                                                                                                                                                                                                                         <  2:167707/250‑1 (MQ=255)                       aaaGGACGTCACATTACCGTGCATTACAGTGGATACCCCCTGCACAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTAGCTTGCTCTGGAGAAAGCTTATAACAGAGGATAACCCCGCATGTTGTTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCGTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTga                                                                                                                                                                                                                   <  2:32834/251‑2 (MQ=255)                            aCGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGtactac                                                                                                                                                                                                              >  1:168848/1‑251 (MQ=255)                                            cAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCtg                                                                                                                                                                                              >  1:372241/1‑251 (MQ=255)                                                 cAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCa                                                                                                                                                                                         <  2:311208/251‑1 (MQ=255)                                                                       tAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAgg                                                                                                                                                                   >  2:262874/1‑251 (MQ=255)                                                                            gaaagtaaaattagacaaaccAGAGACAGCGGCGTGATCTGGCTATGGAGAAAGCTAATAACAAAGGAGAAGCGCGTATGTTGCTTGGTAGACCGCAGACAGACCCGACTCTCGAATGGTTCTTGTCTCATGGCCACATTCATAAGTACCCAGCCAAGAGTAAGCTTAAACACGATGGTGAAAAAG‑CGGAAACGCTGGACGACATCGAAAAAGGCTGAGTGACAGGGCTGAACAAATACGAAGAGAGTaaa                                                                                                                                                              <  2:64084/232‑1 (MQ=255)                                                                             gAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAg                                                                                                                                                             <  1:347903/251‑1 (MQ=255)                                                                                 cATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAAt                                                                                                                                                         <  1:39167/251‑1 (MQ=255)                                                                                               caagagACAGCGGGTTTACCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCAGGGTGCGTGGCAACCCGAAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACAGCGTTAAAGGTTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCATATc                                                                                                                                           <  2:86972/249‑1 (MQ=255)                                                                                                          ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGt                                                                                                                                <  1:407707/251‑1 (MQ=255)                                                                                                            ggTTATCTGTCTCTGGAAAAAGCGAATAACAGAGGATAACGGCGCATGGTGCTTGGCAAACCTCAAACAGACCCGCCTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGAGCCTCTCCTATCTGAATCAGGGTGa                                                                                                                              <  2:257672/250‑1 (MQ=255)                                                                                                                     gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTgg                                                                                                                     <  2:9943/251‑1 (MQ=255)                                                                                                                             gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                                                                                                             >  2:412838/1‑251 (MQ=255)                                                                                                                                         cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATAGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGTTTTTATTGGCGAACTGGGCCTGTTTGa                                                                                                   >  2:153939/1‑249 (MQ=255)                                                                                                                                                 aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑Cgg                                                                                                                                                                                                                            <  2:293261/120‑1 (MQ=255)                                                                                                                                                 aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑Cgg                                                                                                                                                                                                                            >  1:293261/1‑120 (MQ=255)                                                                                                                                                                 tGGCAACCAGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTCCCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCGCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATgg                                                                          <  2:246324/250‑1 (MQ=255)                                                                                                                                                                                   cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc                                                                                            <  1:90220/214‑1 (MQ=255)                                                                                                                                                                                   cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc                                                                                            >  2:90220/1‑214 (MQ=255)                                                                                                                                                                                      gACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAACAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCtgtg                                                    >  2:198576/1‑251 (MQ=255)                                                                                                                                                                                         tctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTAAATCGTTAAAGGCTCTGTGGCAGTGCTGATCGAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGa                                                   >  1:408959/1‑249 (MQ=255)                                                                                                                                                                                                            cATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACa                               >  1:249219/1‑250 (MQ=255)                                                                                                                                                                                                               ggCCACATTAATAAGTCCCCATCCAAGACAAAGCTTATTCACCGGGGTAAAAAGG‑CGAAAACGCTGTACTACAGCGTAAAAGGCTCTGTGGCAGGGCTGATAAAAGACGAAGAGGGAAAAGAAATGATCCTCTCCGATCTGAATCAGGGTGATTTTATTGGCGAACGGGGCCTTTTGGAAGAGGGCAAGGAAGGTAGCGAAGGGGTACGGGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaaaa                           <  2:372241/250‑1 (MQ=255)                                                                                                                                                                                                                gCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTTGGGGGCAGTGCTGATAAAAGACGAGGAGGGTAAAGAAAGGATCCTCTCTTATCGGAATCGGGGTGATTTTATTGGCGAACGGGGCCTTTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTAGGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaaaaa                          <  1:412838/251‑1 (MQ=255)                                                                                                                                                                                                                                cccacccaAGAGCAAGCTTATTCCCCAGGG‑GAAAAAGGCGAAAACGCTGTCCTACATCGTAAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAGGAGGGAAAAAAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACGGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAGGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTc          <  2:249219/251‑1 (MQ=255)                                                                                                                                                                                                                                  cATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCa                                                                                                                                    <  1:98355/127‑1 (MQ=255)                                                                                                                                                                                                                                  cATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCa                                                                                                                                    >  2:98355/1‑127 (MQ=255)                                                                                                                                                                                                                                        agagCAAGCTTATTCACAAGGGTGAAAAAG‑CGGAAACGCTGTACTAAATCGTAAAAGGCTCTGTGGCATGGCTGATAAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTACCTGAATCAGGGTGATTTTATTGGCGAACGGGGCTTGTTGGAAGGGGGCGAGGAACGAAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAc  <  2:170133/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAC  >  NC_000913/3485968‑3486449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑