Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,121	A→G	S283P (TCG→CCG)	yeiB ←	DUF418 domain‑containing protein YeiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,121	0	A	G	100.0%	124.8 / NA	36	S283P (TCG→CCG)	yeiB	DUF418 domain‑containing protein YeiB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

AGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCC  >  NC_000913/2241905‑2242364                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    aGATAACAGCGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTACAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGACAGAAGCCATAAAACAGCGGCGCCTAGCCAATCGCCTGAAACGGCGCACTCAGt                                                                                                                                                                                                                   >  2:190150/1‑251 (MQ=255)             agagGATATTCGCCAGCCATACCGGAATAACAACCGCCAGCAGCTCCAGGCGGTCAAAATGCATCAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGATTGCAATAGATAGTTGGTCAGCGCCATCCGTCAGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAtt                                                                                                                                                                                                       >  1:390507/1‑251 (MQ=255)              gagGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGTCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCATAATCCATAAATCAGCGGCGCATAGCCGATCGCCTGAAACGGCGCACTCATTTCCCGCGGCAttt                                                                                                                                                                                                      <  2:186501/251‑1 (MQ=255)              aagGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAttt                                                                                                                                                                                                      <  2:208877/250‑1 (MQ=255)                           agccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAAGCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGc                                                                                                                                                                                         <  1:253560/251‑1 (MQ=255)                              caTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGAACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGcac                                                                                                                                                                                       >  2:187216/1‑250 (MQ=255)                                         aaCAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACg                                                                                                                                                                           <  1:127347/251‑1 (MQ=255)                                           cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGGGGTACAAATCAGCGTTTGCAATAGATAGTTGGGCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGcc                                                                                                                                                                         <  1:64453/251‑1 (MQ=255)                                                   agcagcTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGGACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCCTTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTcc                                                                                                                                                                   >  2:152811/1‑249 (MQ=255)                                                         tCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGcc                                                                                                                                                            >  2:61515/1‑250 (MQ=255)                                                            aGGCGGTCAAAATGCATAAACAAACCGAGGGGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTg                                                                                                                                                        <  2:312050/251‑1 (MQ=255)                                                              gCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCa                                                                                                                                                      <  2:172828/251‑1 (MQ=255)                                                                    aaaaTGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                                 >  2:391849/1‑250 (MQ=255)                                                                              aacaaacCGAGGCGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGa                                                                                                                                        >  2:23467/1‑249 (MQ=255)                                                                                    ccGAGGCGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAAt                                                                                                                                >  2:75026/1‑251 (MQ=255)                                                                                       aGGTGGTAAAAAAGCGTGGTACAAAGCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGTCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACTCCAGCGAGACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGt                                                                                                                             <  1:198295/251‑1 (MQ=255)                                                                                                 aaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATc                                                                                                                   <  1:11873/251‑1 (MQ=255)                                                                                                        ggTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGccccc                                                                                                             >  2:240055/1‑247 (MQ=255)                                                                                                               aTCAGCGTTTGCAATAGAGAGTGGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCGCAATTGCGGCTAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAGCGGCGCGCTCAGTTCCCGCTGCATTTGAAGTAAGAAGGCACACCAGCTGTGCGCCCAGGCCAGCTGCCATTGCAGGGCGATGTCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACaaa                                                                                                     <  1:255898/251‑1 (MQ=255)                                                                                                                 cAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAAc                                                                                                   >  2:215708/1‑251 (MQ=255)                                                                                                                        tGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCAc                                                                                                                                                                                                                        >  2:25982/1‑127 (MQ=255)                                                                                                                        tGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCAc                                                                                                                                                                                                                        <  1:25982/127‑1 (MQ=255)                                                                                                                         gcaatagctccgtGGTCAGCGCCATCAATCGGAGGCAGGCGATCGCAAGAACCAGCTTAAAGAGGCTCAATTGCGGCCCGAAGCCATAAACCAGCGGCGCATAGCCAATCGCCTGCAACGGCGCACTCAGTTCCAGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCCGCCATTGCAGGGCGATGGCAGGAAGGTTACTGGTCACCCCCATCGCCACCAGCACAAAACCAGGacg                                                                                            >  2:65909/13‑250 (MQ=255)                                                                                                                          cAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTacgac                                                                                          >  1:238149/1‑251 (MQ=255)                                                                                                                                      ggTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCTGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAAGGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGTCGATAGTGACGt                                                                               <  2:382497/250‑1 (MQ=255)                                                                                                                                             gcCATCCGTCAGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGa                                                                        >  1:361525/1‑250 (MQ=255)                                                                                                                                                          cGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                          <  1:220080/251‑1 (MQ=255)                                                                                                                                                          cGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                          <  1:220108/251‑1 (MQ=255)                                                                                                                                                          cGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTcagc                                                          >  1:223666/1‑251 (MQ=255)                                                                                                                                                                  aTCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCt                                                                                                                                                               >  2:301715/1‑142 (MQ=255)                                                                                                                                                                  aTCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCt                                                                                                                                                               <  1:301715/142‑1 (MQ=255)                                                                                                                                                                     gCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcg                                               <  1:360967/251‑1 (MQ=255)                                                                                                                                                                       aaGCACCAGCTTAAAGCGGCTCACTTGCGGCCCGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGACACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcgc                                              >  2:257254/1‑250 (MQ=255)                                                                                                                                                                               gCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCCCAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGcgcc                                     >  2:4786/1‑251 (MQ=255)                                                                                                                                                                                                          aGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATCCGCCCAGTCCAGCTTCCATTGCAGGGCGATGGCAGGAAGGTTAAGGGTCACCCCAAGCGCAACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAGCTGCCCTTTCAGCCAGCCGCGGCGCATCAGCGCCGGACCAAGGAGCATCATCCCTGCCAGt          <  2:81848/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgccagcc  >  2:276122/1‑251 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    AGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCC  >  NC_000913/2241905‑2242364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑