Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,007,727	C→T	E122K (GAG→AAG)	yedD ←	lipoprotein YedD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,007,727	0	C	T	100.0%	88.4 / NA	25	E122K (GAG→AAG)	yedD	lipoprotein YedD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

AAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTCTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATC  >  NC_000913/2007489‑2007935                                                                                                                                                                                                                                               |                                                                                                                                                                                                                 aaaCCATTCATCTGGGAGAGAATTACGGCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGAGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCg                                                                                                                                                                                                      >  1:221046/1‑251 (MQ=255)        tCATCTGGGAGAGAATTACGGCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGAAGCGTCAGCGTGTGGGGTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGGTTTCCTTACGGCAGTGGCGTCGGGAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGTTGTCGGGCGGTccccc                                                                                                                                                                                               >  2:291541/1‑248 (MQ=255)                     aaTTACGGCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCa                                                                                                                                                                                  <  2:77051/251‑1 (MQ=255)                            gCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACCCGCTCCATCGTCATACCATc                                                                                                                                                                           >  2:36753/1‑251 (MQ=255)                                                     ggggAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACCGCGCTTTATCCCGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCTTCATACCATCGTACTACATGGTGTTACCGTCCCGc                                                                                                                                                  >  2:306561/1‑251 (MQ=255)                                                       ggAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGGGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCCGCGCGGCGGAACACTTTGCTGTCGGGCGATCCCCACGCTCCATTGTCATACCAACTTACTCAATGGTGTTACCGTCACGCTc                                                                                                                                                >  2:124114/1‑251 (MQ=255)                                                                aaGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGt                                                                                                                                        >  1:65992/1‑250 (MQ=255)                                                                    gagagTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGc                                                                                                                                   <  1:56361/251‑1 (MQ=255)                                                                       agtagtGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGgtgt                                                                                                                                                             <  1:353602/222‑1 (MQ=255)                                                                       agtagtGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTc                                                                                                                                 >  2:309658/1‑250 (MQ=255)                                                                       agtagtGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACAATTTGCTGTCGGGCGATCCCCACGCTCCATCGTCATACCATCGTACTCAATGgtgt                                                                                                                                                             >  2:353602/1‑222 (MQ=255)                                                                        gtagtGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCAc                                                                                                                               >  1:121433/1‑251 (MQ=255)                                                                                  acgaAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGt                                                                                                                     <  1:36753/251‑1 (MQ=255)                                                                                                           ttGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACAt                                                                                             <  1:298624/250‑1 (MQ=255)                                                                                                                                  tgtgGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCcggcacggc                                                                     >  1:344250/1‑251 (MQ=255)                                                                                                                                              aaGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTg                                                         >  2:386049/1‑251 (MQ=255)                                                                                                                                               agagGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGc                                                        <  2:338317/251‑1 (MQ=255)                                                                                                                                                  ggTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCAc                                                     <  1:217006/251‑1 (MQ=255)                                                                                                                                                         tAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGc                                              >  1:323448/1‑251 (MQ=255)                                                                                                                                                               cATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCa                                        <  2:115282/251‑1 (MQ=255)                                                                                                                                                                  ccccGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAggg                                     >  1:223225/1‑251 (MQ=255)                                                                                                                                                                                cGGGGAGATTGCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACACTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAAGCCAGGGTATCGCCCTCTTTa                       <  2:281450/251‑1 (MQ=255)                                                                                                                                                                                                     gTGGCGCCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCTGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTTCCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGGATCGCCCTCTTTAGTGACAATCATGCTGCCTATc  <  2:259819/251‑1 (MQ=255)                                                                                                                                                                                                                aaCGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCaa                                                                                                                                              >  2:324842/1‑100 (MQ=255)                                                                                                                                                                                                                aaCGGCGCTTTATCCAGCGCGGCGGCACACTTTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCaa                                                                                                                                              <  1:324842/100‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                 AAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGAGTAGTGACCGACGAAAACGGCGAAGCAACCTTCTTTTGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAAATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCACACTCTGCTGTCGGGCGATCCACACGCTCCATCGTCATACCATCGTACTCAATGGTGTTACCGTCACGCTCAACCTCGTACAGCTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATC  >  NC_000913/2007489‑2007935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑