Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,936,057	C→T	L86L (CTG→CTA)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,057	0	C	T	100.0%	86.9 / NA	26	L86L (CTG→CTA)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

CGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGC  >  NC_000913/1935813‑1936303                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGt                                                                                                                                                                                                                                                  <  2:218632/251‑1 (MQ=255)          cGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCGCAGCGGTTTCTCCATGACTCCGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATATTTTTGATCCAGCATCGCGCCGAGTCGGCTGAATGCAGCAGTGTCAGTGACATCGAGATTACAAAAATCTAGACGTGCACTCAg                                                                                                                                                                                                                                          <  2:25017/250‑1 (MQ=255)                    aTCATTGATTTCATGCGAGGTCGCCAGCGACGTCCCCAGTGGATTCTACATGACTACGCGTGCCGGTTTAGAATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAACAATCTAGACGTGCACTCAGGGTGTCCCAt                                                                                                                                                                                                                                >  1:258682/1‑250 (MQ=255)                                    gAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATag                                                                                                                                                                                                                              >  1:273812/1‑235 (MQ=255)                                    gAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTGTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCAGCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGGGTCCCATaa                                                                                                                                                                                                                              <  2:273812/236‑1 (MQ=255)                                         cGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGtttctt                                                                                                                                                                                                          >  1:343045/1‑251 (MQ=255)                                            cAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCTGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGtttctttc                                                                                                                                                                                                        >  1:287082/1‑250 (MQ=255)                                                                 ctcCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTcgc                                                                                                                                                                                  >  1:187156/1‑251 (MQ=255)                                                                            cgcgTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTg                                                                                                                                                                       <  1:358610/251‑1 (MQ=255)                                                                                                      gCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAg                                                                                                                                              <  2:30753/250‑1 (MQ=255)                                                                                                            ccAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGccc                                                                                                                                       >  1:99013/1‑251 (MQ=255)                                                                                                                       gCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATa                                                                                                                             >  1:78642/1‑250 (MQ=255)                                                                                                                        cAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAAt                                                                                                                           <  2:242637/251‑1 (MQ=255)                                                                                                                                            gggCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCt                                                                                                       <  2:78642/251‑1 (MQ=255)                                                                                                                                                      gCAAAGGAGTTAATGGTGAAACGATTTGTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGGGTCATTGACATCGAGATTACAAAAATCTAGACGTGGACTCAGGGTGTCCCATAAACCGTCAACAATGGTTTCTTTCATGAAAGGTTCGAGCGCCTCGCGGACAACTTTGGTATAGGCCGCGTGGTGCCAGTCAGCGCGCCCGACGCCGAGAAGCCGGGTTGCCGGGTTGATCTGACCGTCttt                                                                                             <  2:242012/251‑1 (MQ=255)                                                                                                                                                                                       tCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCAt                                                                                                                                                                                                      <  2:109960/113‑1 (MQ=255)                                                                                                                                                                                       tCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCAt                                                                                                                                                                                                      >  1:109960/1‑113 (MQ=255)                                                                                                                                                                                                            cTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTcgc                                       >  2:351797/1‑251 (MQ=255)                                                                                                                                                                                                              gAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCTAGACGTGCACTCAGTGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCgcgc                                     <  2:9825/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttgagaTTACAAAAATCTAGACGTGCACTCAGGGTGACCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGc               >  1:156845/3‑251 (MQ=255)                                                                                                                                                                                                                                     cGAGATTACCGAAATCTGGACGTGCACTCAGGGTGACCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGCGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGAAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGcc              >  2:372160/1‑251 (MQ=255)                                                                                                                                                                                                                                       agaTTACAAAAATCTAGACGTGCACTCAGGGTGTCCCCTAAACCTTCATCCATGGTTTCTTTCATGCAAGTTTCGAGCGCCTCGCGGACAAATTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATCATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGCTACAGGGAAGGCAGCAATTTACGACGCGCAAgg                                                  >  2:395738/1‑213 (MQ=255)                                                                                                                                                                                                                                       agaTTACAAAAATCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCTATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAgg                                                  <  1:395738/213‑1 (MQ=255)                                                                                                                                                                                                                                           tACAAAAATCTAGACGTGCACTCAGGGTGTCCCATTAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTTCGCCGATAATCCGGGTGTCCGGGTTGAGCTTACCGGCttc                                                                                             <  1:279643/166‑2 (MQ=255)                                                                                                                                                                                                                                           tACAAAAATCTAGACGTGCACTCAGGGGGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGAGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCttc                                                                                             >  2:279643/1‑165 (MQ=255)                                                                                                                                                                                                                                                 aaTCTAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGc  <  2:99013/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGC  >  NC_000913/1935813‑1936303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑