Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,327	C→T	intergenic (+68/‑361)	yobD → / → mntP	DUF986 domain‑containing inner membrane protein YobD/Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,327	0	C	T	100.0%	54.8 / NA	16	intergenic (+68/‑361)	yobD/mntP	DUF986 domain‑containing inner membrane protein YobD/Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/5);  total (11/5)

TCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATAT  >  NC_000913/1905102‑1905571                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     tcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGttattta                                                                                                                                                                                                                                <  2:48271/248‑1 (MQ=255) tcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTatttattt                                                                                                                                                                                                                             >  1:131086/1‑251 (MQ=255)           cTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGCAGCTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGCTCGAAATATCGCAGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTc                                                                                                                                                                                                                    >  1:395654/1‑250 (MQ=255)                   aaTATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGAGTGTTGGAGATGCAATTAGACCAGCGTAGGCTGTTAATCCGAGTTAGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTAAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTATTAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatat                                                                                                                                                                                                            >  1:187359/1‑250 (MQ=255)                                  aagcgctcaaCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTCTTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGaa                                                                                                                                                                                             >  2:111592/9‑250 (MQ=255)                                   aGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAACTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAACTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGaaa                                                                                                                                                                                            >  1:41875/1‑250 (MQ=255)                                                 cGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATaa                                                                                                                                                                              >  1:184270/1‑250 (MQ=255)                                                                                  aGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGtttt                                                                                                                                             >  1:230577/1‑250 (MQ=255)                                                                                                         cGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAg                                                                                                                                                                            <  1:122955/196‑1 (MQ=255)                                                                                                                            aaaaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGt                                                                                                  <  1:366659/251‑1 (MQ=255)                                                                                                                                            tctcGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATATTTCATAATCAGACATAGCTAAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATACAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGAtt                                                                                  >  1:67607/1‑251 (MQ=255)                                                                                                                                                         cAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACg                                                                     >  1:23543/1‑251 (MQ=255)                                                                                                                                                               gTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACGTGTCaa                                                               <  1:217354/251‑1 (MQ=255)                                                                                                                                                                                 gCTATGATTAGTGTATTTTTAATAATCAGACATAGCTTCGGCTATATTATCTCTTCCCTTTTTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACg                                             >  2:264603/1‑251 (MQ=255)                                                                                                                                                                                                           cAGACATAGCTTAGGCTATATTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTtc                    <  1:107573/250‑1 (MQ=255)                                                                                                                                                                                                                            atatTATCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCatat  >  1:22415/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     TCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAATCGTTATCAATAAAGCAATGAAATAATATATTCCAACAGTTGTTTTATATTCTCAAAATATGTTAAGGTTGCGCCCTCATTTGGGGAGTAGCCGATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATAT  >  NC_000913/1905102‑1905571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑