Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,270	G→A	P27S (CCG→TCG)	ydhU ←	putative cytochrome YdhU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,270	0	G	A	100.0%	128.4 / NA	36	P27S (CCG→TCG)	ydhU	putative cytochrome YdhU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

AACCAGACTTTTAACCGCAGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGA  >  NC_000913/1750025‑1750511                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  aaCCAGACTTTTAACCGCAGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCaa                                                                                                                                                                                                                                               >  2:297632/1‑250 (MQ=255)       aCTTTTAACCGCAGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCgg                                                                                                                                                                                                                                        <  1:24820/251‑1 (MQ=255)          tttAACCGCAGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCggg                                                                                                                                                                                                                                       <  2:298311/249‑1 (MQ=255)              aCCGCAGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCCCTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCAGGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGaa                                                                                                                                                                                                                                 <  2:8166/251‑1 (MQ=255)                   aGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACtgtg                                                                                                                                                                                                                            >  2:177452/1‑251 (MQ=255)                   aGTTGCGCCCACCAGCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACtgtg                                                                                                                                                                                                                            <  2:186502/251‑1 (MQ=255)                     ttGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGc                                                                                                                                                                                                                          >  2:302567/1‑251 (MQ=255)                                                  aGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTGGACCAGGGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTg                                                                                                                                                                                             <  2:166163/251‑1 (MQ=255)                                                        cTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGc                                                                                                                                                                                       >  1:215146/1‑251 (MQ=255)                                                                       aCAAAGAGTAACGCATTCGACCAGTGCCATAATCTGCCCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGtt                                                                                                                                                                        >  2:180392/1‑251 (MQ=255)                                                                                  cGCATTAGACCAGTGCCATACTCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTc                                                                                                                                                             >  2:264235/1‑251 (MQ=255)                                                                                                    tAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATTATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATTCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGTAACTGTTCAGCATGTTGCGACGGGTTCCTACATTTTCCTTTTTAATCaatttttt                                                                                                                                           >  1:123497/1‑245 (MQ=255)                                                                                                     aaTCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGa                                                                                                                                          <  1:260449/251‑1 (MQ=255)                                                                                                             cGCTTTGCGGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCTCACCGACCAAAATTCTGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCAGACATTTTCCTTTTTAAGCAAATGTTGACCGAACCg                                                                                                                                  <  2:45154/251‑1 (MQ=255)                                                                                                                   gCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGAt                                                                                                                             >  2:68723/1‑250 (MQ=255)                                                                                                                        aaaGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATAc                                                                                                                           >  1:211291/1‑247 (MQ=255)                                                                                                                                       cACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTg                                                                                                                                           <  1:403339/216‑1 (MQ=255)                                                                                                                                       cACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTg                                                                                                                                           >  2:403339/1‑216 (MQ=255)                                                                                                                                         ccATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTc                                                                                                                                                                       <  1:50047/186‑1 (MQ=255)                                                                                                                                         ccATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTc                                                                                                                                                                       >  2:50047/1‑186 (MQ=255)                                                                                                                                         ccATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                                      <  1:35654/251‑1 (MQ=255)                                                                                                                                           aagATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCACGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAgg                                                                                                    >  2:330213/3‑251 (MQ=255)                                                                                                                                            tGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGt                                                                                                   >  1:407040/1‑251 (MQ=255)                                                                                                                                             gATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGt                                                                                                   <  2:215146/250‑1 (MQ=255)                                                                                                                                                  gTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGa                                                                                             <  1:182911/251‑1 (MQ=255)                                                                                                                                                                           cACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCa                                                                    <  1:297632/251‑1 (MQ=255)                                                                                                                                                                                    aGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCTGCACCAGGTAGCTGATATTGATAGTATTTATTGTGCTGCAACCACGCCt                                                           <  1:396990/251‑1 (MQ=255)                                                                                                                                                                                           cAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCaaa                                                                                                                                                <  2:70581/159‑1 (MQ=255)                                                                                                                                                                                           cAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCaaa                                                                                                                                                >  1:70581/1‑159 (MQ=255)                                                                                                                                                                                                           cacaTCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTcgcg                                     >  2:296029/1‑250 (MQ=255)                                                                                                                                                                                                           cacaTCACAACCAGCAATACTCCGGCAATGATCAACCACACCGACAAAAATTCGGGAGTAAACTGTGGCACATAGTTCGATAACTGGCTCTGAAACTGTTCAGCATGTTGCGAAGGGCTAATACCTTTTCCTTTTTAATCAAATGCTGACCGAACCGTCGATACAAATGCGGTTTCCCGGCACCAGGTAACGTATATTGATAGTATTTATTTTGCTGCAACCAATCCTGAATGTCTAGGCTATCTTcgcg                                     >  2:296031/1‑250 (MQ=255)                                                                                                                                                                                                             caTCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGAcc                                  >  2:137207/1‑251 (MQ=255)                                                                                                                                                                                                              aTCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGAcc                                  >  1:97712/1‑250 (MQ=255)                                                                                                                                                                                                                    aCCAGCAATACTCCGGCAATGATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCaa                                >  2:220642/1‑246 (MQ=255)                                                                                                                                                                                                                                        gATCAACCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGgc       >  1:176439/1‑251 (MQ=255)                                                                                                                                                                                                                                             aCCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGTGCAGGCGCTGa  <  1:300654/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  AACCAGACTTTTAACCGCAGTTGCGCCCACCATCGCAAAGTGATTTATCAGCCCACTGGCCAGCAACAATACAAAGAGTAACGCATTCGACCAGTGCCATAATCTGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGA  >  NC_000913/1750025‑1750511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑