Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,700,308	C→T	R318R (CGC→CGT)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,308	0	C	T	100.0%	115.4 / NA	33	R318R (CGC→CGT)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/21);  total (12/21)

CATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATG  >  NC_000913/1700072‑1700551                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    cATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCTGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAgg                                                                                                                                                                                                                                       <  1:311772/251‑1 (MQ=255)    cacaTTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTc                                                                                                                                                                                                                                     <  1:401613/250‑1 (MQ=255)                         cGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGc                                                                                                                                                                                                               <  1:221318/251‑1 (MQ=255)                                       aGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTggcg                                                                                                                                                                                                 >  1:28625/1‑251 (MQ=255)                                        ggcggcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAACGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTggcgg                                                                                                                                                                                                <  2:352301/251‑1 (MQ=255)                                              aCGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTAc                                                                                                                                                                                          <  1:364147/251‑1 (MQ=255)                                                 cAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCg                                                                                                                                                                                        >  1:36686/1‑250 (MQ=255)                                                            cGGTCAAACCGGCAGAGGCTCTCGGACCATCTTCCAGGTGCACTTGCGTTGCCCGGCCGCTCACTGTTTTTCTTCAAGCGCCGCGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTGTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAt                                                                                                                                                                            <  2:336345/251‑1 (MQ=255)                                                               tCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAttcc                                                                                                                                                                         >  2:408869/1‑251 (MQ=255)                                                                         aGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTcctgttcctgtt                                                                                                                                                               <  1:309315/251‑1 (MQ=255)                                                                                                     aaTTGAGTTGCCCGGCCACTCACGGTTTTTCTGAGAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCTCCCGGAAAATCGTCATAAAATTAAAGGGCTGCTGATTTCTGGCCTGATCGCCTGCGGCGTTGGCGGCACTACCGATGCGCTGGAATGCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCAc                                                                                                                                   <  2:49225/251‑1 (MQ=255)                                                                                                          aGTTGCCCGACCGCTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCt                                                                                                                              <  2:360192/251‑1 (MQ=255)                                                                                                               cccGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATTGTCATAAAATTAAAGGTCTGCTGATTTCTGGc                                                                                                                                                                                                                       >  2:279284/1‑157 (MQ=255)                                                                                                               cccGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGc                                                                                                                                                                                                                       <  1:279284/157‑1 (MQ=255)                                                                                                                   aCCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCTCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCCGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAACTCGTCCTAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTATCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCgg                                                                                                                     >  1:82354/1‑251 (MQ=255)                                                                                                                         cACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTc                                                                                                                 <  2:36686/249‑1 (MQ=255)                                                                                                                              tttttCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTTTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTc                                                                                                           >  2:316474/1‑250 (MQ=255)                                                                                                                                tttCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCAc                                                                                                         <  1:143554/250‑1 (MQ=255)                                                                                                                                     gAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCAt                                                                                                   <  2:28625/251‑1 (MQ=255)                                                                                                                                               cgcgTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTc                                                                                         >  2:346192/1‑251 (MQ=255)                                                                                                                                                                    aaaTGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATAcc                                                                    >  1:318182/1‑251 (MQ=255)                                                                                                                                                                      aTGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGa                                                                  <  1:105353/251‑1 (MQ=255)                                                                                                                                                                                      gcggTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGAc                                                  <  1:147747/251‑1 (MQ=255)                                                                                                                                                                                                    gCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCgg                                    <  1:165555/251‑1 (MQ=255)                                                                                                                                                                                                                aTGTATCACTGCGCGCGCCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGTCTTCACCTTCATGTCTGTGCTCGGCGTCACCTTCGGTAATGCCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCAt                          <  1:410487/249‑1 (MQ=255)                                                                                                                                                                                                                                gcCCGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTgg        <  2:152904/251‑1 (MQ=255)                                                                                                                                                                                                                                 cccGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCACCCAAGTGGt       >  1:59601/1‑251 (MQ=255)                                                                                                                                                                                                                                 cccGGAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGt       <  1:404837/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggAAAATCGTCATAAAATTACAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTCACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATAc                                                                     >  2:96154/1‑186 (MQ=255)                                                                                                                                                                                                                                    ggAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCGGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATAc                                                                     <  1:96154/186‑1 (MQ=255)                                                                                                                                                                                                                                     gAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACAt   <  2:211386/251‑1 (MQ=255)                                                                                                                                                                                                                                     gAAAATCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACAt   >  1:211386/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaaaTCGTCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATg  >  2:274802/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    CATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATG  >  NC_000913/1700072‑1700551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑