Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,755	C→T	A90T (GCG→ACG)	yahC ←	uncharacterized protein YahC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,755	0	C	T	100.0%	50.0 / NA	17	A90T (GCG→ACG)	yahC	uncharacterized protein YahC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

CATTTTTGAATAACTTTTAATACCCGTCGCGTTTAGTCGTCGCTTCGCTTAATTTTTGTCCACCATATTCTGAAATTACCCCCAC‑GCAGTTACCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGC  >  NC_000913/334509‑334980                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  taTTTTTAAATATCTTTTATTACCCGTCGCGTTTAGTCGTCCCTTCGCTTAATTTTTGCCCACCACATCCGGAAATTCCCCCCAC‑CCAGTTCCCCGCCAGAAAACCCATTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCGTGCCAGACCTCTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCa                                                                                                                                                                                                                                <  1:330692/249‑1 (MQ=255)                             gcgTTTAGTCGTCGCTTCGCTTAATTTTTGTCCACAATATTCTGAAATTACCCACAC‑GCAGTTACCCGCCAGAAAACACAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTAATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGgcc                                                                                                                                                                                                    >  1:241249/1‑250 (MQ=255)                                         cgcttcgcttAATTTTTGTCCACCATATTCTGAAATTACCCCCAC‑GCAGTTACCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACcagc                                                                                                                                                                                         >  1:319041/1‑249 (MQ=255)                                          gtttcgcttAATTTTTGTCCCCCATATTCT‑AAATTCCCCCCCCCGCAGTTCCCCGCCAGAAAACCCAGAAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCGGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGt                                                                                                                                                                                      <  2:24876/249‑1 (MQ=255)                                            ttcgcttAATTTTTGTCCACCATATTCTGAAATTACCCCCAC‑GCAGTTACCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACaga                                                                                                                                                                                                        >  2:39507/1‑231 (MQ=255)                                            ttcgcttAATTTTTGCCCACCATATTCTAAAATTACCCCCAC‑GCAGTTACCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACaga                                                                                                                                                                                                        <  1:39507/231‑1 (MQ=255)                                                             caccaTATTCTGAAATTACCCCCAC‑GCAGTTACCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTAGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGcc                                                                                                                                                                   >  2:197322/1‑251 (MQ=255)                                                                              ccccccAC‑TAAGTTCCCCGCCAGAAAGCCCAATAAAGTCGCCGTCCGGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAGGCCCCCGGGAATAAAGTACGGTAACAAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACAGCATCTCGGaaga                                                                                                                                                  <  2:240161/250‑3 (MQ=255)                                                                                            tACCCGCCAGAAAACCAAGTAACGTCACCGTCCAGTCCATCCCGCGGGCAAAAAACAGCGTCATGCGGAGAAAGCCAACGGGAATAAAGGACAGAAACCAGAAACGCCGTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGAAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGCGGCCAGTCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTACCCGCCAg                                                                                                                                      <  1:375608/250‑1 (MQ=255)                                                                                                                   gtccccgcccAGTTCATCCCGCTGGCAAAAAACAGCGTCATCCCGAAAAAGCCACCGGAAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTAcc                                                                                                              <  1:364354/243‑1 (MQ=255)                                                                                                                             aGTTCATCCCGCTGGCAAAAAAAAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACATCTGATAAACCCCGATTTACCGCCGCCTGCg                                                                                                    >  2:185247/1‑251 (MQ=255)                                                                                                                                          ggCAAAAAACAGCGTCATGCCGAGAAAGCCCCCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGc                                                                                       <  1:197322/251‑1 (MQ=255)                                                                                                                                              aaaaaCAGCGTCATGCCGAGAAAGCCACCGGGAATAAGGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCACCCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCaaaa                                                                                   <  1:162630/251‑1 (MQ=255)                                                                                                                                                       gTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTACCCAGAAACGCCCGTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAAc                                                                                                                                                                      <  2:408583/159‑1 (MQ=255)                                                                                                                                                       gTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAAc                                                                                                                                                                      >  1:408583/1‑159 (MQ=255)                                                                                                                                                                                                         ttGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGa                          >  2:85138/1‑249 (MQ=255)                                                                                                                                                                                                                               aaGGGTACGGTAGTAATTACGCTCGTCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACTCCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGGCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGCCATGTGAAGACACTAACTGCCAAAGCCCTGc  <  1:205767/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  CATTTTTGAATAACTTTTAATACCCGTCGCGTTTAGTCGTCGCTTCGCTTAATTTTTGTCCACCATATTCTGAAATTACCCCCAC‑GCAGTTACCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTTGCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGC  >  NC_000913/334509‑334980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑