Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,077,466 G→A L473L (CTG→TTG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,077,4660GA100.0% 85.3 / NA 26L473L (CTG→TTG) putAfused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/16);  total (10/16)

GCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGTTTGCCGTCGGCAACTTTCCCGGTGACCTGCTCATACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCG  >  NC_000913/1077220‑1077711
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
gCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGTTTGCCGTCGGCAACTTTCCCGGTGACCTGCTCATACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACttt                                                                                                                                                                                                                                                   <  1:304452/251‑1 (MQ=255)
      gaaaCGCCAACAGCGTTTCATGTGTGCAAACCGGGGAAAAAATACGACACGGACGGTTAAGTTTGCCGTCGGCAACTTTCCCGGGGACCTGCTCATACAGTGGCTCGCCCATCCCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCGGCCCCGCCAGTTGATAAAGCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGca                                                                                                                                                                                                                                             <  2:59847/248‑1 (MQ=255)
         cccccagcAGCGTTTCATGTGGGCCAACCGGAGAATAAATACGAAACGGACGGTTAAGTTTGCCGTCGGCAACTTCCCCGGTCACCTGCTCATCAAGTGGCTCGCCCATCCCAGGCAGGCACTGGACCTCGTCCTGCCCCGGGTAGTATTTCTTCCCCGCAAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAg                                                                                                                                                                                                                                          <  2:303169/244‑1 (MQ=255)
                             gtgCCAACCGGAGCATAAATACGACACGGACGGTTAAGTTTGCCGGCGGCAACTTTCCCGGTGACCTGCTCATACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCgg                                                                                                                                                                                                                       <  1:245275/250‑1 (MQ=255)
                                ccaaacggggaaTAAATACGACGCGGACGGTTAAGTTTGACGTCGGCAAGTACCCCGGTGACCTGCTCATACAGTGGCGCGCCCAGACCAGGCAGGCAAGGGAACTCTGACGGACCCGGGTAGTAGTTCTGCGCAGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGtata                                                                                                                                                                                                                   <  2:61568/240‑1 (MQ=255)
                                                                                    tCCCGGTGACCTGCTCATACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGGAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACCCCTTGCGGGTATAAACCGGATAACCTTCCAGGCCGTCCATCTGCGCACGc                                                                                                                                                               >  2:135747/1‑251 (MQ=255)
                                                                                         gggACCTGCTCATACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCATTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAAt                                                                                                                                                          <  1:391754/249‑1 (MQ=255)
                                                                                                   catccagtggtccGCCCATACCATGCAGGCCCTGGAACTCGTCCTGCCCCGGTAAGAGGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATc                                                                                                                                                 <  2:267980/238‑1 (MQ=255)
                                                                                                     tACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATcccc                                                                                                                                              >  1:380864/1‑250 (MQ=255)
                                                                                                                                 aCTGGAACTCGTCCTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAatcctc                                                                                                                  >  2:246006/1‑248 (MQ=255)
                                                                                                                                   tGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAatcatc                                                                                                                  >  1:167470/1‑249 (MQ=255)
                                                                                                                                           gTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAgacggcgac                                                                                                        >  1:219086/1‑251 (MQ=255)
                                                                                                                                             aCTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCa                                                                                                                                                                          >  1:361692/1‑183 (MQ=255)
                                                                                                                                                gACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATaca                                                                                                                                                                                                                 >  2:40156/1‑141 (MQ=255)
                                                                                                                                                gACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAAGCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTGGAGTAGATTCGGCTCCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATaca                                                                                                                                                                                                                 <  1:40156/141‑1 (MQ=255)
                                                                                                                                                                     ccGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAgg                                                                              <  1:137578/251‑1 (MQ=255)
                                                                                                                                                                              gATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGaaa                                                                                                                                                                                                                             >  1:111174/1‑99 (MQ=255)
                                                                                                                                                                              gATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGaaa                                                                                                                                                                                                                             <  2:111174/99‑1 (MQ=255)
                                                                                                                                                                                   aTCGCCGCCAGCGTAGGGGCGTTTTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCaa                                                                <  1:246006/251‑1 (MQ=255)
                                                                                                                                                                                                            gCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGa                                       <  2:167470/251‑1 (MQ=255)
                                                                                                                                                                                                                cgcgATCTGCGGTTAGATTAGATTCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACCCCTTGCGGGTATAAACCGGATAACCTTCAATGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATaa                                   <  1:1331/251‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGGTAGATTAGAGCCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAAc                             <  1:185936/249‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGGTAGATGAGATTCGGCACCGCAAGCAACTTTTTCGCACAGGCGAGATAAGAACCGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTAGCCCAGTACGCGACTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCCCCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAAc                             <  1:185963/249‑1 (MQ=255)
                                                                                                                                                                                                                                       tCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTgc                                                                                                                                                                     <  1:122154/98‑1 (MQ=255)
                                                                                                                                                                                                                                       tCGGCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTgc                                                                                                                                                                     >  2:122154/1‑98 (MQ=255)
                                                                                                                                                                                                                                         ggCACCGCCAGCAACTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGc          >  1:179359/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 cagcaaCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACTCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGTCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCg  <  2:161166/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
GCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAAATACGACACGGACGGTTAAGTTTGCCGTCGGCAACTTTCCCGGTGACCTGCTCATACAGTGGCTCGCCCATACCATGCAGGCACTGGAACTCGTACTGACCCGGGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCG  >  NC_000913/1077220‑1077711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑