Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,065,815	G→A	V77V (GTG→GTA)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,065,815	0	G	A	93.9%	101.8 / ‑4.7	34	V77V (GTG→GTA)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (17/14);  total (19/15)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCG  >  NC_000913/1065585‑1066059                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aTGAACAACACGCTAATCGCCGCAGCTGTGGCAGGGAAAGATTTACTCGCTTCAAAAACTCAGGCACAAACCGTACCGGAAAGCTATCAGCTACAACAAGTGCTCCTGCTGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGGGTATATGGGcc                                                                                                                                                                                                                                   >  2:238503/1‑250 (MQ=255)         aaCGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGc                                                                                                                                                                                                                          >  1:164019/1‑251 (MQ=255)                    ccgcAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAAtggctg                                                                                                                                                                                                               <  1:37506/251‑1 (MQ=255)                     cgcAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTg                                                                                                                                                                                                                       >  1:153517/1‑242 (MQ=255)                     cgcAGCTGGGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTg                                                                                                                                                                                                                       <  2:153517/242‑1 (MQ=255)                            gtgGCAGGGATAGTTTTACTCGCTTCAAACGCCCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGCGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGGGGGCAACTCACCACCAAAGGGGGCGGACTCGAAGTGTATATGGGCCATTACATGCGGGAATGGCTGGCCGAGc                                                                                                                                                                                                        >  2:142042/1‑250 (MQ=255)                                                    taaaaCGCCCAGGCATAACCCGTCCCGGAAGGCTACCAGCTACAGAAAGTGCTCATTATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAAt                                                                                                                                                                                <  1:125776/248‑1 (MQ=255)                                                     aaaaCGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGc                                                                                                                                                                              <  2:129416/250‑1 (MQ=255)                                                       aaCGCTCAGGCAAAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGccc                                                                                                                                                                            >  1:311644/1‑251 (MQ=255)                                                           cTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACCGCAAGTGCTCCTGATGAGCCGCCATAACTTACGTGCACCGCTGCCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGAGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccgccg                                                                                                                                                                        >  1:235718/1‑251 (MQ=255)                                                                      aCCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGt                                                                                                                                                             >  1:78250/1‑251 (MQ=255)                                                                                           tgaaagcaagtgccaatgtggggccgccATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTc                                                                                                                                         <  2:212962/228‑1 (MQ=255)                                                                                            tACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTc                                                                                                                                         >  1:212962/1‑249 (MQ=255)                                                                                                gCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAAc                                                                                                                                   <  1:210744/251‑1 (MQ=255)                                                                                                                            ttACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGAc                                                                                                                      >  2:312929/1‑236 (MQ=255)                                                                                                                            ttACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGAc                                                                                                                      <  1:312929/236‑1 (MQ=255)                                                                                                                                          ggCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCAtt                                                                                           <  2:10895/249‑1 (MQ=255)                                                                                                                                                           tgCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCt                                                                        >  2:206391/1‑251 (MQ=255)                                                                                                                                                             cTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCtgt                                                                      >  2:340299/1‑251 (MQ=255)                                                                                                                                                                gAGCATTCGACGCCGAATAAATGACCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGACGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGCAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGc                                                                    >  2:63024/1‑250 (MQ=255)                                                                                                                                                                        gACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAgg                                                           <  1:297598/251‑1 (MQ=255)                                                                                                                                                                          cGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaa                                                         <  2:164019/251‑1 (MQ=255)                                                                                                                                                                               aaTAAATGGCCAGAATGGGACGTCCACGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAAt                                                    <  1:149703/251‑1 (MQ=255)                                                                                                                                                                                    aTGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCTCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCTCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGcc                                               >  2:286359/1‑250 (MQ=255)                                                                                                                                                                                      ggCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACt                                             >  1:263415/1‑251 (MQ=255)                                                                                                                                                                                                  aCGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAAcc                                 <  2:195643/251‑1 (MQ=255)                                                                                                                                                                                                       cccGGTGGGCAACTCACCACCAAAGGTGGCGTACTCGAAGTGTAGATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTaa                            <  2:311644/251‑1 (MQ=255)                                                                                                                                                                                                          ggTGGGCAACTCACCACCAACGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGCATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTCTTACCGGAGCAGTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAAAGGGCACTATTGAACCAACCTTTAAccc                         >  2:302011/1‑251 (MQ=255)                                                                                                                                                                                                                cAACTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGAt                   <  1:198496/251‑1 (MQ=255)                                                                                                                                                                                                                  aCTCACCACCAAAGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGGGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATc                  >  2:76391/1‑250 (MQ=255)                                                                                                                                                                                                                             aaGGTGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTcc      >  1:213646/1‑251 (MQ=255)                                                                                                                                                                                                                             aaGGTGACGTGCTCGACATGCATATGGGCTATTACATGCGAGAATGGCTGGCAGAGCAGGGGCTGGAGAAATCGGGGGAATGACCGCCGCCGAACACCGTTTATGACTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGCTCTTTATTACCGGCGAATTACCGGGGAGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCAAACCTTTAATCCGGTGATCACCGATGAtttc      >  2:391434/1‑249 (MQ=255)                                                                                                                                                                                                                                gttgCGTTCTCGAAGTGTAGATAGGCCATTACATGCGTGAATGGCGGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATTCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGGGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTccgcc   <  2:198609/248‑1 (MQ=255)                                                                                                                                                                                                                                 tGGCGTACTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTccgccg  >  1:230832/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCG  >  NC_000913/1065585‑1066059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑