Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,889	C→T	L404F (CTC→TTC)	fdrA →	putative oxamate carbamoyltransferase FdrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,889	0	C	T	100.0%	159.4 / NA	48	L404F (CTC→TTC)	fdrA	putative oxamate carbamoyltransferase FdrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/22);  total (26/22)

AGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATCTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCT  >  NC_000913/547664‑548124                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ggcagcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATGGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTGAATTGCCGATTTCGGCTCTGAACCGCAAGGGCGCgt                                                                                                                                                                                                                    <  2:255491/250‑1 (MQ=255) agcagcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGGTTAATTTCCGGACACCTTTGCGTGGGAGCCGCCGATACCCCTCAAAATTGCCTGATGCTGGGCGCCCACAGACACCCGATTATTTACCCCGGCGGTGGTTTTTACACCCTCGGGCGGCCCCCTTCCAT‑GATCGACCCCACC‑CTTCCCAACCCGGTTATTTCCGATTTCGGCGCTAAACCGCCAGGGcgcgg                                                                                                                                                                                                                    >  1:116244/1‑250 (MQ=255)     gcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACATGCCGGACCCCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTtg                                                                                                                                                                                                                <  2:364567/251‑1 (MQ=255)                       tGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTTCCGGACACCTTGGCGTGGGAGCCGACGATAACCCTCAACAAGGCATGATGCTGGGCGCCGATAGCCCCCAGATTATTTACCTCGGCGGAGATTTCTACACCGTCCGGCGGCCCCATCCGCT‑GATCGACCCAACC‑TTACGCAACCAGTTAAATTCCGATTTCGGCGCTAAACCGCCAGTGCGCGTGTTGCTGCTTGATGGCGTggtt                                                                                                                                                                                              >  1:57400/1‑248 (MQ=255)                             ccGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTTCCGGACACCCTGGCGTGGAAGCCGACGATACCCCTCAACATGGCCTGATGCTGGACGCCGATAGCCACCCGATTATTGACCTCGGCGATGATTTCTACACCCTCCGGCGTCCCCCTCCGAT‑GATCGACCCCACC‑TTTCGCAACCCGTTAATTTCCGCTTTCCGCGCTAACCCCCCAATGCGCGTGTTTTTGCTTGGTGTCGTGATTGGCTTc                                                                                                                                                                                        >  2:292544/1‑251 (MQ=255)                              cggcggTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCg                                                                                                                                                                                       <  1:90969/251‑1 (MQ=255)                                 cggTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATg                                                                                                                                                                                                      <  1:1188/233‑1 (MQ=255)                                             tgCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCCTCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTTGCTTCGGTGCGACCGCCGATc                                                                                                                                                                        >  1:70807/1‑251 (MQ=255)                                                            aTTACTTGCCGGACCCCTTGGGGTGGAAGCCAACGATCCCCATCAAAATGGCATGTTGCTGGACGCCGATAGCCACCAGATTTTTGACCTCGGGGATGATTTCTACACCGTCGGGCGCCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAGGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTGCGGTGCGACCGCCGATCCTGCCGCCTCGCTgg                                                                                                                                                         <  2:363076/251‑1 (MQ=255)                                                                    ccGGACACCCTGGCGCGGAACCCGCCGATCCGCATCACCTTGGCATGATGCTGGACGCCGCCAGCTACCAGTTTATTGACCTCGGCGATGATTTCTCAACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCACCCAGTTCATTGCCGATTTCGGCGCTAAACCGCAAGTGCCCGAGTTCCTGCTTGATGTCGTGCTTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGcc                                                                                                                                                 >  2:204041/1‑251 (MQ=255)                                                                       gACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTgg                                                                                                                                              >  1:278874/1‑251 (MQ=255)                                                                       gACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTgg                                                                                                                                              >  2:100906/1‑251 (MQ=255)                                                                         cacatgggCGTGGAGGCCGACGATCCCCACCACCATGGAATGATGCTGACCGCCGATAGCCACCGGATGATTGTCCCCGGCGATGATTTCTACCCCGTCGGCCTGCCCCATCCGAT‑GATCGCCCCAACC‑TTACGCAACCATTTAATTGCGGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGTGGTCGTGATTGGCTTCGGTGCGCCCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCa                                                                                                                                            <  2:71011/245‑1 (MQ=255)                                                                                           gacgaTACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCcgcgcg                                                                                                                          >  1:205759/1‑251 (MQ=255)                                                                                               aTACCCATCAACAGGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGGCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTGGGCGCAAAACCGCAAGGGCGCGTGTGGCTGCTTGAGGTCGTGATGGGCTTCGGTGCGACCGCCGAGCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTa                                                                                                                      <  1:95476/251‑1 (MQ=255)                                                                                                     aTCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAAt                                                                                                                >  1:301559/1‑251 (MQ=255)                                                                                                      tCAACATGGAATGATGCGGGAAGCCGTTAACCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCCACC‑TTAAGCAACCTGTTAATTGCCGCTTTCGGCGCTAAACCGCCAGAGCGCGTGTTGCTGCTTGATGTAGTGCTTGGCTACGGCGCGCCCGCCGATCCTGCCGCCTCGCTGGCGAGCGCCTGGCAAAAAGGCTGTGCCGCACGTTTACATAATc                                                                                                               >  2:188222/1‑251 (MQ=255)                                                                                                      ccAACATGGCATGATGCGGGCCGCCGTTGGCCACCAGATTATTGCCCTCGGCGATGATTTCTACCCCGTCGGGCGCCCCCATCCGAT‑GATCGCCCCAACC‑TTACGCACCCAGTTAATGGCCGATTTAGGCGCTAACCCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATc                                                                                                               <  2:278874/250‑1 (MQ=255)                                                                                                      acAACATGGCATGATGCGGGACGCCGATACCCACCAGATGATTGCCCGCGGCTATGATTTCTCCACCGTCGGGCGTCCCCA‑CCGATTGATCGCCCCACCC‑TTCCGCACCCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGGGGGTGGCTGTTTGATGTTTTGTTTGGCTCCGGGGCGTCCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTATATAATc                                                                                                               <  2:262961/250‑1 (MQ=255)                                                                                                          cATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAAcc                                                                                                           >  2:215168/1‑251 (MQ=255)                                                                                                               cATGATGCTGGACGCCGATAGCCCCCAGATTATTGCCCTCGGCGATGATTTCTACCCCGTCGGGCGTCCCCATCCGAT‑GATCGCCCCACCC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGt                                                                                                      <  1:110725/251‑1 (MQ=255)                                                                                                                atgatgCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTgg                                                                                                                                              >  2:372039/1‑210 (MQ=255)                                                                                                                atgatgCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTgg                                                                                                                                              <  1:372039/210‑1 (MQ=255)                                                                                                                    ggctggcccccaaTAGCCCCCAGATTATTGCCCCCGGGGTTGATTTCTACCCCGCGGGGCTTCCCCATCGGAT‑GTTCG‑CCCAACCCTTCCGCACCCATTTAATGGCCGTTTTCGGCGCTAACCCGCAAGGGGGCGTGTTGCTGCTGGATGGCGTGATTGGCTTGGGGGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGc                                                                                                  <  2:166249/238‑1 (MQ=255)                                                                                                                      cTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGAGCCGCGCGTTTAGATAATCACCAACTGTATGCCAt                                                                                               >  2:397155/1‑251 (MQ=255)                                                                                                                        ggACGCCGATCGCCACCAGATTATTGACCTCGGCGATGCTTTCTACACAGTCGGGCGCCCCCATCCGAT‑GATCGCCCCAACC‑TTACGCAACCAGTTAACTGCCGATTTCGGCGCTACCCCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTTTGCCGCGCGTTTAGATAATCAACCACTGTATGCCAttt                                                                                             >  2:184037/1‑250 (MQ=255)                                                                                                                        ccgctcgtccgatctccaGATTATTGCCCCCGGCAATTATTTCTACACCGTCGGGCGCCCCCATCCGAT‑GATCGACCAACCC‑TGCCGCACCCAGTTAATTGCCGATTTCGGCGCAAACCCGCAAGTGCGCGGGTTGCTGTTTGATGTCGTGATGGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTg                                                                                             <  2:241294/236‑1 (MQ=255)                                                                                                                         gACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGc                                                                                            >  2:382253/1‑251 (MQ=255)                                                                                                                          ccGCCGATACCCACCAGATTATTGACCGCGGCTATGATTTCTACACCTGCGGGCGTCCCCATCCGAT‑GCTCGACCCAACC‑TTACGCAACCAGTTAATTGCCGTTTTGGGCCCTAACCCGCAAGGGCGCGTGTTGCGGCTTGAGGTCGTGTTTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGcc                                                                                           <  1:384843/250‑1 (MQ=255)                                                                                                                           cGCCGATTGCCACCAGATTATTGACCTCGGCGATGCTTTCTACACCGTCGCGCGTCCCCCTCCGAT‑GCTCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGCTCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAACAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGcc                                                                                           >  2:40762/1‑250 (MQ=255)                                                                                                                           cGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGcc                                                                                           >  1:49818/1‑250 (MQ=255)                                                                                                                               gagaGCCACCAGATTATTGCCCCCGGCGTTGATTTCTCCACGGCCGGGCGTCCCCACCCGAT‑GATCGACCCAACC‑TGACGCACCCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGTTTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGt                                                                                      <  2:307345/248‑1 (MQ=255)                                                                                                                                    ccaccaGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGAc                                                                                   >  2:260204/1‑249 (MQ=255)                                                                                                                                       ccaGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGTTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGc                                                                               >  2:284806/1‑250 (MQ=255)                                                                                                                                       ccaGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTg                                                                                             >  1:241294/1‑236 (MQ=255)                                                                                                                                                       ggCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGAt                                                                                                                                                                                                       <  2:157087/114‑1 (MQ=255)                                                                                                                                                       ggCGATGATTTCTACACCGTCGGGAGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGAt                                                                                                                                                                                                       >  1:157087/1‑114 (MQ=255)                                                                                                                                                        tgatgtttttCTACACCGTCGGGCGTCCCCATCAGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGAAAAAAGCCTGTGCAGCGCGTTTAGATAATCAACCACTGTATGCCATTGCAACGGTGACAGGCACTGAACGTGACCCGCa                                                              >  1:344293/8‑250 (MQ=255)                                                                                                                                                         cGATGATTTCTACCCCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCACCCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATGGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAAt                                                            <  1:284806/251‑1 (MQ=255)                                                                                                                                                          gatgctTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCGCGCTGGTGAGAGCCTGGCAACAAGCCTGTGCCGCGCGGTCAGATAATCAACCAATGTATGCCATTGCCACGGTGACACGCACTGAACGTGACCCGCAATg                                                           >  2:15929/1‑251 (MQ=255)                                                                                                                                                                 tCTACACCGTCGAGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAAAGTGACCCGCAATGCCGCTcc                                                    >  2:412721/1‑250 (MQ=255)                                                                                                                                                                         gtcggcggTCCCCCTCCGAT‑GATCGACCCACCC‑TTACGCAACCAGTTAATTGCCTATTTCGGCTCTAAGCCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGGGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAAt                                            <  1:341029/244‑1 (MQ=255)                                                                                                                                                                            gggCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGc                                         >  2:331454/1‑251 (MQ=255)                                                                                                                                                                              gcttCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCTTGTTGCTGCTTGATGTCGTGTTTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCa                                       <  1:351191/248‑1 (MQ=255)                                                                                                                                                                                   cccATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAACCCGCAAGGGCGCGTGTTGCTGCTTGATGTCGTGATTGGTTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTg                                  <  1:123856/251‑1 (MQ=255)                                                                                                                                                                                      aTCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGaa                               <  1:119769/251‑1 (MQ=255)                                                                                                                                                                                                 cGACCCACCC‑TTACGCAACCAGTTAATTGCCGATTTCGGCGCTAACCCGCAGGTGGGCGTGTGGCTGTTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGa                     <  2:70807/251‑1 (MQ=255)                                                                                                                                                                                                                     cAGTTAATTGCCGATTTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCt  >  2:397161/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            AGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGAT‑GATCGACCCAACC‑TTACGCAACCAGTTAATTGCCGATCTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCT  >  NC_000913/547664‑548124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑