Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	463,208	C→T	P432S (CCG→TCG)	ppiD →	periplasmic folding chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	463,208	0	C	T	100.0%	176.6 / NA	51	P432S (CCG→TCG)	ppiD	periplasmic folding chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

GACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTG  >  NC_000913/462974‑463444                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               gacgacATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTAt                                                                                                                                                                                                                                >  1:312788/1‑251 (MQ=255)   cgacATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCt                                                                                                                                                                                                                              <  2:384931/251‑1 (MQ=255)     acATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCttt                                                                                                                                                                                                                            <  1:291652/251‑1 (MQ=255)            gccagcGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAAcgg                                                                                                                                                                                                                       >  1:279948/1‑249 (MQ=255)                                  gACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGGAGGTGAAAACGgcgc                                                                                                                                                                                               >  1:172024/1‑251 (MQ=255)                                   aCGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGgcgc‑g                                                                                                                                                                                             <  1:335944/251‑1 (MQ=255)                                       aGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCgg                                                                                                                                                                                         <  1:239763/251‑1 (MQ=255)                                            gTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGGAGGGGAAAACGGCGC‑GCCCGGCAt                                                                                                                                                                                     >  2:31763/1‑250 (MQ=255)                                                cgacATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTTTGGTAGGGGAAAACGGCGCCGCCggcctcaaat                                                                                                                                                                                 >  2:25180/1‑243 (MQ=255)                                                                  gAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTa                                                                                                                                                              <  2:127132/250‑1 (MQ=255)                                                                    aaCACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGGAAGGGAAAACGGCGC‑CCCCGGCAT‑CCACTCTGGcatcctcccccgcgac                                                                                                                                                           >  2:211905/1‑239 (MQ=255)                                                                       acGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGGAGGTGAAAACGGCGC‑GCCCGGCAT‑CAACTCTGACCTCATCACCGTAGACggg                                                                                                                                                        >  2:253667/1‑250 (MQ=255)                                                                                      aTGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGtg                                                                                                                                         <  1:72969/251‑1 (MQ=255)                                                                                       tGCGTACTACGCGCTGCAGCAGAAAGGGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACTTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCCGGCAT‑CAAATATGGCATCATCAccatcgaacgcgcacccccattcgtgg                                                                                                                                        >  2:286098/1‑226 (MQ=255)                                                                                         cGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGTTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGtt                                                                                                                                                                                                                                           <  2:197087/152‑1 (MQ=255)                                                                                         cGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGtt                                                                                                                                                                                                                                           >  1:197087/1‑152 (MQ=255)                                                                                          gTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGGCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGtgctgc                                                                                                                                     <  2:406933/251‑1 (MQ=255)                                                                                            actacGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACg                                                                                                                                                                                                   <  2:196739/189‑1 (MQ=255)                                                                                            acgacGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCACCGACACAGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTgcg                                                                                                                                    <  2:366811/247‑1 (MQ=255)                                                                                                   gcTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGGGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACCTCATTACCGTTGACGGCGACCCCGCATTCGTGCTGCGCATCcgcg                                                                                                                            >  2:114399/1‑251 (MQ=255)                                                                                                    cTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCACAGATAACCTGCCGGAAGCGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTATGGTAGGTGAAAAAGGCGC‑GGCGGGCAT‑CAACTCTGACATCATCACCGCAGACAGCGACCGCGCACTCGTGCTGCGCATCAGCg                                                                                                                            >  1:366342/1‑250 (MQ=255)                                                                                                     tGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGGGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCAATCGCGCTGCGCATCAGCg                                                                                                                            >  1:345432/1‑249 (MQ=255)                                                                                                      gcagcaGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGa                                                                                                                           <  1:211905/249‑1 (MQ=255)                                                                                                       cagcagACAGGGAGCGATGCGACAAGCAACAACACCGAGTCTCTGGCCGGAGCCGAGCAAGCTGCCGGCGTTAACGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGACTGGTTGGGGAAAACGGCGC‑CCCGGGCAT‑CAACTCTGACATCATCACCGAAGACGGCCACCGCGCCTTCGTGCTGCCCATCcgcgcga                                                                                                                         >  2:271795/1‑247 (MQ=255)                                                                                                          cagAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGACTTGTAGGTGGAAAAGGCGC‑GCCGCGGATACAA‑TCATACACCATCACCGGCGACGGGGACCGCCCAATCGTGGTGCGCATCCGCGAGcaca                                                                                                                      >  2:317870/1‑250 (MQ=255)                                                                                                                  gagtgATGCGGCAAGCAACTACACCGCGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCTAAGATAACCGGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGTTATCTTTAACGGAGGTCTGGTAGGTGAAAACTGCGC‑GCCGGGCAT‑CATCGCTGACATCAGCACCTTAGACGGCGACCGCGCATACGTGCTGCTCATCGGCGTGCACATGCCGGAAg                                                                                                             <  2:44384/247‑1 (MQ=255)                                                                                                                       aTGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTg                                                                                                        <  1:358683/251‑1 (MQ=255)                                                                                                                         gCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGa                                                                                                       <  2:71502/250‑1 (MQ=255)                                                                                                                           ggCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAAACGGAAGCGGGGaaa                                                                                                     >  1:14434/1‑250 (MQ=255)                                                                                                                           ggCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGGAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCCCCCTCGGCGGCGACCGCGCATTCGTGCTCCGCATCAGCGAGCACAAACCCGGAGCGGGGAAAc                                                                                                    >  2:51085/1‑251 (MQ=255)                                                                                                                                     gACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGACTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGGAGCGGGGAAACCGTTGGCaa                                                                                           >  1:141864/1‑249 (MQ=255)                                                                                                                                           gAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTATGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGAAATCATCACCGTTGAAGGGGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGGGAAAACGTTGGCCGATGTTc                                                                                     >  1:363482/1‑250 (MQ=255)                                                                                                                                                           cAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGACGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGATACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCa                                                                    <  2:279948/251‑1 (MQ=255)                                                                                                                                                            agagCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CCACTCTGACATCATCACCGTAGACGGCGACCGCGCCTTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGGGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCAt                                                                   >  2:241000/1‑251 (MQ=255)                                                                                                                                                            agagCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCCTTCGTGCTGCGCATCAGCGAGCACCAACCGGGAGCGGGGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCAt                                                                   >  1:308371/1‑251 (MQ=255)                                                                                                                                                             gagCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCAtt                                                                  <  1:381555/251‑1 (MQ=255)                                                                                                                                                                            gTTAAAGCCACTCAGGCGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCAcaacaa                                                   <  1:146262/251‑1 (MQ=255)                                                                                                                                                                              aaaaGCCACTCAGACAGGTTGGTTCAGCAAAGATAACCAGCCGGAAGAGTTGAACATCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGAGGTTCAGGAACAAGTTAAGGCATTGGTACTGCACAACAAAg                                                 <  2:24572/250‑1 (MQ=255)                                                                                                                                                                               aaaGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTGGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCATCACAACAAAGc                                                <  2:349944/251‑1 (MQ=255)                                                                                                                                                                                aaGCCAATCAGACGGGTTGGTTAAGCCAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGCCGCTATCTTTAACGGCGGTCTGGAAGGGGAAAACGGCGC‑GCCGGGCCT‑CAACTCTGAACTCATCACCGTCGAAGGCGCCCGCGCCGTCGTGCTTCGCCTCAGCGAGCACCAACCGGAAGCGGGGGAACCGCTGGGCGATGTTCCGGAACAAGTTAAGGCACTGGATCacaaccaaaaagct                                               >  2:63059/1‑238 (MQ=255)                                                                                                                                                                                   ccACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTCGGGGAAAACGGGGC‑GGCGGGGAT‑CCAATCTGACATCCTCACCGTAGACGGCGACCGCGCCTTCGTGCTGCGCATCAGCGAGCACAAACCGGAAACGGGGAAACCCTTGGGCGGGGTTCAGGAACAAGGTTAGGCATTGGTTCCGCACAACAAAGCTgc                                             >  2:237439/1‑249 (MQ=255)                                                                                                                                                                                      cTCAGACGGGTTGGTTCAGCACAGCTAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCCGGGCGGCGAAAACGGCGC‑GCCGGGCAT‑CAAATCTGACATCATCCCCGGAGAAGGCGACCGCGCATTCGTGCTGCGCATCACCGGGCAAAAACAGGGAGCGGGGAAACCGTTGGGAGAAGTTCAGGAACAAGTTAAGGCATGGGGTCAGAAAACCAAAGCTGaaac                                          >  2:201831/1‑248 (MQ=255)                                                                                                                                                                                        cAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGaacaac                                        >  1:106013/1‑250 (MQ=255)                                                                                                                                                                                        cAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAacaaca                                       <  1:253667/251‑1 (MQ=255)                                                                                                                                                                                          gACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAgg                                     <  1:133563/251‑1 (MQ=255)                                                                                                                                                                                                  ggttCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGt                              <  1:31838/250‑1 (MQ=255)                                                                                                                                                                                                       aGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGc                        <  1:145128/251‑1 (MQ=255)                                                                                                                                                                                                                      ccGGAAGAGTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGt         <  2:312788/251‑1 (MQ=255)                                                                                                                                                                                                                           agagTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGAtt    <  1:241000/251‑1 (MQ=255)                                                                                                                                                                                                                           agagTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAAACGGAAGAGGGGAAACCGTTGGCAGAAGTTCAGGAAAAAGTTAAGGCATTTGGTCAGCACAACAAAGCTGAACAAAAGGCGAAAGGGGATGCTGAGAAACTGCTGGGTGAtt    >  2:351374/1‑251 (MQ=255)                                                                                                                                                                                                                             agTTGAACTTCAAGTCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGGGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGGGGAAGCTGAGAAACTGCTGGGTGAtttt  >  1:158916/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               GACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAGGTGAAAACGGCGC‑GCCGGGCAT‑CAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTG  >  NC_000913/462974‑463444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑