Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	446,923	C→T	A261A (GCG→GCA)	cyoE ←	heme O synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	446,923	0	C	T	100.0%	64.7 / NA	20	A261A (GCG→GCA)	cyoE	heme O synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/7);  total (13/7)

ACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGTAATCCTTAAAGCGGAAAATGG  >  NC_000913/446691‑447164                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  aCTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGtcatca                                                                                                                                                                                                                                 <  1:339054/251‑1 (MQ=255)                                     cGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAaccac                                                                                                                                                                                             >  2:255436/1‑251 (MQ=255)                                                          cGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCgcggcg                                                                                                                                                                        >  1:67240/1‑251 (MQ=255)                                                                                 tAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGtcat                                                                                                                                                                                                                                   <  2:321387/169‑1 (MQ=255)                                                                                 tAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGtcat                                                                                                                                                                                                                                   >  1:321387/1‑169 (MQ=255)                                                                                             tGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCa                                                                                                                                       >  1:23745/1‑249 (MQ=255)                                                                                              gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTCCCACAAAGCAGCCAGCAGCGTATGCGAGTCGGGTACCATAAAATCACCGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTGGCGTGCCCAGATTCTGTAATCAGCAACTTTATAACCGCGCAGAGCAATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAaga                                                                                                                                    <  1:255436/251‑1 (MQ=255)                                                                                                                     agGTTTTTTTACCCAACAGCACCCAGCAGCTTATGCGGGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCACCTTTATAACCGCGCAGAGCCATACCAAACCCCCAGCCGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGc                                                                                                             <  1:372013/251‑1 (MQ=255)                                                                                                                                             agcagcGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATCCCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTta                                                                                                                                                         <  1:122610/183‑1 (MQ=255)                                                                                                                                             agcagcGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTta                                                                                                                                                         >  2:122610/1‑183 (MQ=255)                                                                                                                                                              cGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTCTACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAg                                                                                                         >  2:305809/1‑214 (MQ=255)                                                                                                                                                                            cAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTAcc                                                      >  2:164138/1‑251 (MQ=255)                                                                                                                                                                               cGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGatat                                                                                                                                                            <  1:59989/146‑1 (MQ=255)                                                                                                                                                                               cGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGatat                                                                                                                                                            >  2:59989/1‑146 (MQ=255)                                                                                                                                                                                  aCATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCg                                                                          >  2:26447/1‑225 (MQ=255)                                                                                                                                                                                  aCATCACCCCCGAGAGGGCAGTGAGGGCGATGATAGAGAAGCCGAACAGCTGGCGTGCCCAGATTCTGTCATCAGCAACTTTATACCCGCGCAGAGCCATCCCAAACCCCCAGACGCAAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCg                                                                          <  1:26447/225‑1 (MQ=255)                                                                                                                                                                                    atcatcACCGAGAGGGCAGTGATGGCGCTGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCa                                               >  1:98529/1‑250 (MQ=255)                                                                                                                                                                                                 gggCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGACTGttt                                 >  1:384141/1‑251 (MQ=255)                                                                                                                                                                                                    cAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTgcc                              >  1:3662/1‑251 (MQ=255)                                                                                                                                                                                                                                cAGCTTGCGTGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACAAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGTAATCCTTAAAGCGGAAAATgg  >  1:140367/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  ACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTCATCAGCAACTTTATAACCGCGCAGAGCCATACCTAACCACCAGACGCTAACCGCCGCGGCGACCACCAGATATTTATACCCAGCGTAACCGCCAAGAGAGAGCATCAGCGTGGCAACGGCAAAGGCGATGATATACAGCGTGATGTGATTCTTCGCCACCGAAATGCCTTTTACCACTGGCAATACCGGAATGTTTGCCGCCTGGTAATCCTTAAAGCGGAAAATGG  >  NC_000913/446691‑447164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑