Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	370,161	C→T	R517R (CGC→CGT)	mhpA →	3‑(3‑hydroxyphenyl)propanoate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	370,161	0	C	T	100.0%	147.2 / NA	43	R517R (CGC→CGT)	mhpA	3‑(3‑hydroxyphenyl)propanoate hydroxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

CGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGATTGCCAGCGCCCGCGAGCGTATTGCGGCATTCTC  >  NC_000913/369918‑370401                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cgcgATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTttgttg                                                                                                                                                                                                                                           <  1:390180/251‑1 (MQ=255)     aTCGGGGCGAACGTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGATGAGTGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACAGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCCAGGGCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATTGTTTTGTTgcc                                                                                                                                                                                                                                         <  2:245502/249‑1 (MQ=255)       cGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTgccgcc                                                                                                                                                                                                                                      >  1:282571/1‑250 (MQ=255)                      ggTAATTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCaa                                                                                                                                                                                                                       >  1:382836/1‑250 (MQ=255)                         aaTTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCTTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCTTTTTGTTGCCGCCACCGCCATTCCGCAAAccc                                                                                                                                                                                                                   <  1:248596/251‑1 (MQ=255)                         aaTTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAGGTGCAAATTCATACCGCACAGGATACCCAGGACGGCGTATTACGCGTGGGCGATCGGCAAGGGCGCCTGCGTAGCGGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAAccc                                                                                                                                                                                                                   <  1:248585/251‑1 (MQ=255)                             ggCTGGGGATGAAATCCACGGTGGGGGATGGGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACCCGCTTCAG‑TCAGGTGGTGCCGGAAGTGCAAATTCATCCCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGAAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGAGGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTggg                                                                                                                                                                                                               <  1:381978/251‑1 (MQ=255)                                 ggggATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCa                                                                                                                                                                                                             >  1:43944/1‑249 (MQ=255)                                  gggATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGGGGGGCGCGTGGGGCACACGCTTCAT‑CCAGGGGGGGCCGGAAGTGCAAATTCATCCCGCACAGGATAACCGCTAGGGCGTACTACGCGTGGGCGATACGCAAGTGCTCTTTCGTAGGTGTTTCGGGCAACAGAAGTCTTGGCTTGTGTGGATGCTCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGa                                                                                                                                                                                                          <  1:184339/251‑1 (MQ=255)                                    gATGCAATCAACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGAcc                                                                                                                                                                                                        >  1:129965/1‑251 (MQ=255)                                     aTGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACAGGCTTCAT‑GCAGGTGGTGCCGGAAGGGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATCCGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGGGGTAATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGAccc                                                                                                                                                                                                       <  1:291342/251‑1 (MQ=255)                                     aTGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGAccc                                                                                                                                                                                                       >  2:23301/1‑251 (MQ=255)                                              aCTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAAc                                                                                                                                                                                              >  1:70933/1‑251 (MQ=255)                                                           aGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGtgat                                                                                                                                                                                 >  2:363717/1‑251 (MQ=255)                                                                 gggcaaagccggcggTGGCGGGCGGTGGGCACCCGCTT‑ATGTCAGTTGGTGCGGGAGGTGAAAATTCATACCGCAAAGGATAACCCCGACGGCGTACTACGCGTGGGCGATCCGCAAGGTCGCCTGCGGAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTAATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCt                                                                                                                                                                           <  2:37761/237‑1 (MQ=255)                                                                    cAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTgccg                                                                                                                                                                                                                                        <  1:50151/187‑1 (MQ=255)                                                                    cAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTgccg                                                                                                                                                                                                                                        >  2:50151/1‑187 (MQ=255)                                                                           agcagTGGCGCGCGTTGGGCACACCCTTCAT‑TCAGGTGGTGCCGGAAGAGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCt                                                                                                                                                                 >  2:27899/1‑251 (MQ=255)                                                                                        gTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCg                                                                                                                                                                                                                          >  1:157200/1‑181 (MQ=255)                                                                                        gTTGGGCACACGCTTCAT‑GCAGGTGGGGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAGGGTCGCCGGCGTAGCTGGTTCGCGCAACACAAGGCTTCGCGGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCg                                                                                                                                                                                                                          <  2:157200/181‑1 (MQ=255)                                                                                                          tgcagggggggCCGAAAGTGCAAATACATACCGCACAGGAAAACCACGACGGCGTACGACGCGGGGGCGATACGCAAGGTCGCTTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCTTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAg                                                                                                                                     <  2:84151/239‑1 (MQ=255)                                                                                                                tggtgCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGatat                                                                                                                             >  1:337351/1‑251 (MQ=255)                                                                                                                 ggtgCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATg                                                                                                                            >  2:272911/1‑251 (MQ=255)                                                                                                                     ccGGAAGTGCAAATTCATACCGCACAGGATACCCCCGCCGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACg                                                                                                                        <  1:119788/251‑1 (MQ=255)                                                                                                                            tGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCt                                                                                                                 >  2:170013/1‑251 (MQ=255)                                                                                                                             gCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGGGGGCGATACGCAGGGTCGCCGGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCtt                                                                                                                <  1:266860/251‑1 (MQ=255)                                                                                                                              cAAATTCATACCGCACGGGCTAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGCGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTc                                                                                                               >  2:268707/1‑251 (MQ=255)                                                                                                                                          gCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTttgttg                                                                                                                                                                                                                                           <  1:356968/115‑1 (MQ=255)                                                                                                                                          gCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTttgttg                                                                                                                                                                                                                                           >  2:356968/1‑115 (MQ=255)                                                                                                                                               ggatacccccgagggggtgctacGCGTGGGGGATACGCAGGGTCGCCTGCGTAGCGGGTTCCCGCAACACAATGCTCCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTcg                                                                                              <  2:247045/232‑1 (MQ=255)                                                                                                                                                           cgGCGTACTACGCGTGGGCGATACGCAAGGTCGCATGCGTAGCTAGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCAGGATCGTTTTGATGCCGCCAACGCCATTCCGCAAAACCTGGGAAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGACGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCATTACCACTCGCCGCTGGTgggg                                                                                  >  1:34902/1‑251 (MQ=255)                                                                                                                                                                              gATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCa                                                                 >  1:276196/1‑249 (MQ=255)                                                                                                                                                                                   gCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACATAATGCTTCGTTGGGGGTGATGCGCCCGGATCGTTTTTTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTg                                                          <  1:82892/251‑1 (MQ=255)                                                                                                                                                                                      gggtcgccggcggggcgggTTCGCGCAACAAAAGGCTTCGCGGGGGGGGAGGCGCCGGGACCGTTTTGTGGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACGGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATAGGCACGCTTATCTTCACTGTCGTTCCCACTCGACGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTc                                                       <  2:150702/234‑1 (MQ=255)                                                                                                                                                                                        gTCGCCTGCGTAGCTGGTTCGCGCACCACAATGCTTCGCGGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGa                                                     <  1:13966/251‑1 (MQ=255)                                                                                                                                                                                          cGCCTGCGTAGCTGGTTCGCGCACCACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATg                                                   >  1:103256/1‑251 (MQ=255)                                                                                                                                                                                                           cgcgCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGAtt                                  <  1:112966/251‑1 (MQ=255)                                                                                                                                                                                                                           tCGCTGGTGGTGATGCGCCCGGATCGTTTTTTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGATTGCCAGCGCCCGCGAg                   <  1:272621/250‑1 (MQ=255)                                                                                                                                                                                                                            cGCTGGTGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTTTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGCTTGCCAGCGCCCGCGAGCg                 >  2:230270/1‑251 (MQ=255)                                                                                                                                                                                                                             gCTGGGGGTGATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGATTGCCAGCGCCCGCGAGCGt                <  1:23301/251‑1 (MQ=255)                                                                                                                                                                                                                                ggtggtGATGCTCCGGGATCTTTTTGTTACCCCCCCCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGGGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCTCTCTCCGCTGGTTGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTTAATGGCGTTATTGCCAGCGCCCGCTAGCGTa               <  2:370402/249‑1 (MQ=255)                                                                                                                                                                                                                                      gATGCGCCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGCTGAGGTCAATGGCGTGATTGCCAGCGCCCGCGAGCGGATTGCGGcc       >  2:320863/1‑250 (MQ=255)                                                                                                                                                                                                                                           gcCCGGATCGTTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGATTGCCAGCGCCCGCGAGCGTATTGCGGCATtctc  >  1:103384/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCAT‑TCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGTCAATGGCGTGATTGCCAGCGCCCGCGAGCGTATTGCGGCATTCTC  >  NC_000913/369918‑370401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑