Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,699	0	A	C	62.5%	‑1.7 / 53.8	48	S63A (TCA→GCA)	tfaQ	putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base A (9/9);  new base C (13/17);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTT  >  NC_000913/1634452‑1634866                                                                                                                                                                                                                                                           |                                                                                                                                                                        gccctatGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCAGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTACCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑CAACCGTTTTACCCCGA‑TGATCTTCAACGCGATGCCACGATGACtc                                                                                                                                                                      >  2:335821/5‑251 (MQ=9)   actatGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAACTAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGATATGCCACGCTGACtcat                                                                                                                                                                    >  2:337514/3‑251 (MQ=9)            aCTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTg                                                                                                                                                            >  1:307677/1‑250 (MQ=31)                    ccTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGcc                                                                                                                                                   <  2:307677/251‑1 (MQ=31)                     cTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGcc                                                                                                                                                   >  2:31954/1‑250 (MQ=31)                      tGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCAc                                                                                                                                                 >  2:253166/1‑251 (MQ=11)                        cATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTATGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCAca                                                                                                                                                >  2:206780/1‑249 (MQ=11)                                 tttttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCGGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACTTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAagccagcc                                                                                                                                       <  1:306553/249‑1 (MQ=11)                                      ttGTTTCGGCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCGCTTCTGATATTCCCCGCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGTAAG‑CCATGTCAT‑AGACGGTTTTACCCCGT‑TGTGCTGCAACGAGATGCCACGATGCCTCTGCACTGTTGAAAGCAGCCACAAAGCCAGCCGGAat                                                                                                                                  <  1:373340/250‑1 (MQ=11)                                         tttCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCt                                                                                                                              >  2:301122/1‑251 (MQ=11)                                              tcCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTgg                                                                                                                            <  1:108017/248‑1 (MQ=11)                                                cGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCgggg                                                                                                                       >  2:21624/1‑249 (MQ=11)                                                   cTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCACTCACGGCGATAACAAGGTAAAATTTTCCGGTAACAGACCGAGTACAGAACTAAATAACGCGTCGCCGGACG‑CCACGTCAT‑AGACGGTTTTAACCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCAGGCggagaa                                                                                                                     >  1:195995/1‑246 (MQ=11)                                                      ccggtgccGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTCCCACTTCTGATATTCCCCCCCCGGCGATAACCAGGTAAAATTTTCCGGTAACTGACCGAGTTCAGAAAGAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACTGTTTTAACCCGACTG‑TCGTCAACGAGAGGCCACGAGGCCGCGTCACTTTTGAAAACAGCCTCAAAGTCTGCGGTATTATCTGGCGGTCCAatat                                                                                                                 <  2:195995/245‑1 (MQ=2)                                                            ccGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAc                                                                                                           <  1:180374/251‑1 (MQ=11)                                                               gAACAGTTTTTCTGCTTCCGTATCCTTCACCCCGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGt                                                                                                                                                              >  2:243865/1‑197 (MQ=11)                                                               gAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGt                                                                                                                                                              <  1:243865/197‑1 (MQ=11)                                                                aaCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTAACCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGtt                                                                                                       >  2:16049/1‑251 (MQ=11)                                                                 aCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGttt                                                                                                      <  1:187973/251‑1 (MQ=11)                                                                            tcTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCCCTTCTGCAACTCCCCTTCCGGCGCTCCCCCGCTAACATTTTCCCGTAACGGACCCCGCCCCGCCCTAAATAACGCGTCGCCGCAAG‑CCACGTCACCACACC‑TTTTACACAGA‑TGATCTTCAACGAGATGCCCCTCTGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTTTCGGTGCAATATCGGTaacgtccgccggcagacc                                                                                           >  1:214512/2‑234 (MQ=2)                                                                            gCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAcc                                                                                           <  1:253166/251‑1 (MQ=11)                                                                               tCCGTATCCTTCACCCAGGCTGTGCCGTTCCCCTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAccg                                                                                          <  2:314653/249‑2 (MQ=11)                                                                               tCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAcc                                                                                           <  1:271509/248‑1 (MQ=11)                                                                               tCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAcc                                                                                           >  2:271509/1‑248 (MQ=11)                                                                                  gTATCCTTCACCCAGGCTGGGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATGTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCGGGCAGACCTGTATg                                                                                     <  2:247755/251‑1 (MQ=14)                                                                                    aTCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAATACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAg                                                                                   <  1:253272/251‑1 (MQ=14)                                                                                           aCCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAat                                                                            <  2:35164/251‑1 (MQ=11)                                                                                                ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTTAAAGGGACCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGATGACCA‑GTCAT‑AAACCGTTTTACCCCGA‑TGATCGTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTGCTGTTTGCTGGCAGACCTGTATGaagaggaagagatg                                                                       <  1:335821/251‑14 (MQ=11)                                                                                                     tgCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGcgtc                                                                   >  1:208113/1‑247 (MQ=11)                                                                                                      gCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCa                                                                  <  1:133175/250‑1 (MQ=11)                                                                                                      gCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGAc                                                                                            <  2:36256/224‑1 (MQ=31)                                                                                                      gCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGAc                                                                                            >  1:36256/1‑224 (MQ=31)                                                                                                            ccACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCa                                                            <  1:64893/250‑1 (MQ=11)                                                                                                              aCTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCa                                                         <  2:121145/251‑1 (MQ=31)                                                                                                                     aaaCTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAag                                                                                                                                             >  2:222112/1‑160 (MQ=32)                                                                                                                     aaaCTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAag                                                                                                                                             <  1:222112/160‑1 (MQ=32)                                                                                                                      tattCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGCAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTc                                                 >  2:205893/4‑251 (MQ=11)                                                                                                                                        aaCCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCcagca                                <  1:406341/250‑1 (MQ=31)                                                                                                                                            aGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAAGATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAAGATATGCATCACCTTCACCAAAAAATTCATTAGTTCCGGCCAGCAGATta                           <  2:133293/251‑1 (MQ=14)                                                                                                                                                             aaCGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTcc           <  2:208113/250‑1 (MQ=11)                                                                                                                                                             aaCGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCg          <  2:106104/251‑1 (MQ=11)                                                                                                                                                             aaCGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCg          <  1:98141/251‑1 (MQ=11)                                                                                                                                                             aaCGGACAGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGAAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTc            >  1:304103/1‑249 (MQ=14)                                                                                                                                                               cGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTg        >  1:187924/1‑251 (MQ=11)                                                                                                                                                               cGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTg        >  1:3385/1‑251 (MQ=31)                                                                                                                                                                     gAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgttgtt  >  2:152950/1‑251 (MQ=11)                                                                                                                                                                             aaataaatAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGc                                                                                                                      >  1:164480/1‑127 (MQ=255)                                                                                                                                                                             aaataaatAACGCGTCGCCGGAAG‑CCACGTCAT‑AGACGGTTTTACCCCGA‑TGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGc                                                                                                                      <  2:164480/127‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                        GCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAG‑CCACGTCAT‑AAACCGTTTTACCCCGA‑TGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTT  >  NC_000913/1634452‑1634866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑