Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,269,876	IS5 (+) +4 bp	intergenic (+309/‑100)	lpxT → / → mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2269876 =	0 (0.000)	22 (0.570)	20/472	0.9	100%	intergenic (+309/‑103)	lpxT/mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
	?	NC_000913	2288919 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 2269879	0 (0.000)	31 (0.800)	31/474	0.4	100%	intergenic (+312/‑100)	lpxT/mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AACAATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTT  >  NC_000913/2288673‑2289153                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           aaCAATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGAGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCCGCTGGATATCTTTTTTCCGGACAACACCATCAACTGTCTCACCATACTCAAAAGTTGATGCCAATGCTATGGGTGAATATaaggaa                                                                                                                                                                                                                                         >  1:275160/1‑250 (MQ=255)                                                        atttACTGATTCCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATAGTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACGCAGTCATTGCAGTTCTTCAt                                                                                                                                                                                 <  2:23536/248‑1 (MQ=255)                                                                 tACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCACCTGGATATCTTTTTTCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGTTGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCCGTCATTTCTTTTCTTCATGTTTTAGcc                                                                                                                                                                        >  2:8600/1‑251 (MQ=39)                                                                 tACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTTTCGGCTGACTCAGTCATTTCATTTTTTCATGTTTGAGcc                                                                                                                                                                        >  1:42721/1‑251 (MQ=255)                                                                             aTTTCAGTATTCTTACTAACACCAAATTATTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCAGCTGGGTATTTTTTTCCCGGACAAAACCCTCCACTGTCTCACCCTACTCAACAGTTGATGCCAATGCTATGGGTGAATATTAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCCTTTCATTTCTTCTTGTTTTAGCCGGTTTTTTCTcc                                                                                                                                                            >  2:192912/1‑251 (MQ=37)                                                                                            cttacAACAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGCTTTTCGTATTATTGTCGGTCCCATAAACACCCTGGAGAACTTTTTCCCGGAGAAAACCCTCCCCTGTCTCAACATAATCAACAGTTGTTGCCCATGCTATGGGTGAATATAAGGAAGGTGCGAATAAACGGGGGAATTCTTCTCGGCCGAATCAGTCCTATCATTTCTTCATTTTTGAGCCGATTTTTTCTCCCGTCAATTCCTTGac                                                                                                                                             >  2:275827/1‑250 (MQ=255)                                                                                                                    tGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCACCCGGGTATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGGGAATAAGCGGGGAAATTCTTTTCGGCTGACTCAGTCATTTTCTTTCTTCCTGTTTTAGCCGATTTTTTTTCCCGTAAATGCCTTTGATCAGCCTTTTTAGACCGTttcttt                                                                                                                     >  2:242926/1‑250 (MQ=255)                                                                                                                    tGTTCTACGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAACCCAGCCGGGGATCTTTTTTCCGGACAACACCATCCACTGTCTCACCATACCCAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGGGGGAAAAAGCGGGGGAATTCTTTTCGGCTGACTCAGTCATTTCATTTCTTTATGTTTGAGCCGCTTTTTTCTCCCGTAAAACCCTTGGATTAGCCTATTTAGGCCCTttcttt                                                                                                                     >  2:242908/1‑250 (MQ=255)                                                                                                                            gCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTCAACCCGCTGGGTATCTTTTTCCAGGACAACCACATCCACTGTCTCCCCCTACTCCACAGTTTATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGACTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTTAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAtttt                                                                                                             >  2:251659/1‑250 (MQ=18)                                                                                                                             ccAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa                                                                                                            <  2:47589/251‑1 (MQ=255)                                                                                                                               aGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg                                                                                                          <  1:78884/251‑1 (MQ=255)                                                                                                                                                     gCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTAATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                     >  1:310452/1‑250 (MQ=255)                                                                                                                                                                                ttttccgcgACATCACCATCGACTGTCTCACAATCCTCAACAGTTGCTGCCAATGCTACGGGCGCATCTAAGGAAGGTGCGAATCAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGACGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTCTCTTTTgg                                                         >  2:248789/9‑251 (MQ=14)                                                                                                                                                                                ttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCAtt                                                            <  1:185810/248‑1 (MQ=255)                                                                                                                                                                                      aGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTGCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTGCTTCGCCATTTAAGGCGTTATCCCCAGTTTTGAGTGTGATCTCTCCCACTGACGTATCATTTGGTCCGcc                                                   <  1:192912/251‑1 (MQ=255)                                                                                                                                                                                             cacCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAAc                                             >  2:300452/1‑250 (MQ=255)                                                                                                                                                                                                            cACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtca                                                                                                                                                                                               >  1:234574/1‑89 (MQ=255)                                                                                                                                                                                                            cACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtca                                                                                                                                                                                               <  2:234574/89‑1 (MQ=255)                                                                                                                                                                                                            cACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGa                             <  1:18668/251‑1 (MQ=255)                                                                                                                                                                                                              ccATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGACTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGGATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAAt                           <  1:367505/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTGCTATGGGCGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGt     >  1:75837/1‑251 (MQ=21)                                                                                                                                                                                                                                       cTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGtttt  >  1:401644/1‑251 (MQ=35)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           AACAATTTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTT  >  NC_000913/2288673‑2289153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.