Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	57,276	IS2 (+) +5 bp	intergenic (‑167/‑84)	lptD ← / → djlA	lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	57276 =	0 (0.000)	25 (0.650)	23/474	0.7	100%	intergenic (‑167/‑88)	lptD/djlA	lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
	?	NC_000913	= 382590	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 57280	0 (0.000)	41 (1.060)	40/474	0.2	100%	intergenic (‑171/‑84)	lptD/djlA	lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
	?	NC_000913	381260 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

AAACAGCGCCTGACGCTCACGCTGGTTGGCGAACCACGCCATTTTACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/57525‑57276 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGC  <  NC_000913/382590‑382345                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  AAACAGCGCCTGACGCTCACGCTGGTTGGCGAACCACGCCATTTTACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGT                                                                                                                                                                                                                                                       <  2:363364/251‑1                        GGTTGGCGAACCACGCCATTTTACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCA                                                                                                                                                                                                                                  <  1:133290/249‑1                          TTGGCGAACCACGCCATTTTACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGAC                                                                                                                                                                                                                               >  2:321562/1‑250                                   CACGCCATTTTACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACCCTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGAATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCAACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATC                                                                                                                                                                                                                     >  1:382937/1‑251                                      GCCATTTAACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACT                                                                                                                                                                                                                  >  1:147920/1‑251                                                                     TATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATAT                                                                                                                                                                                   >  2:91338/1‑251                                                                        GGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGT                                                                                                                                                                                                                                                       >  1:48230/1‑180                                                                        GGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGT                                                                                                                                                                                                                                                       <  2:48230/180‑1                                                                             ATTAACAGGCCTAACAATACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATCCTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGG                                                                                                                                                                           >  2:233800/1‑251                                                                                             CTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGC                                                                                                                                                           >  1:251720/1‑251                                                                                                                GCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATT                                                                                                                                        <  1:268806/251‑1                                                                                                                                ACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTTGTGGATTTGCCCCTATATTTCCAGACATCTGTGATCACTTAACCCATTACAAGCCCTCTGCCGCAGATATTCCCGTGGGGAGCGATAACCCAGCGCACTATGCGGATGGCATTCGTTATAATtttcgaa                                                                                                                        <  2:382937/251‑8                                                                                                                                    CCACGCCAATGAGTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATGCCCGTGGCGAGCTATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCTAACGCC                                                                                                                    <  1:163576/251‑1                                                                                                                                                  TTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTT                                                                                                      >  1:74434/1‑251                                                                                                                                                        CAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGC                                                                                                >  1:412980/1‑251                                                                                                                                                                    TATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTC                                                                                    >  1:216177/1‑251                                                                                                                                                                       TCCCCAACTCGACCCACGCATATCAGGGCCATCTCCCACAATATAAAGGTGCTTTTACCGATTTCCGGCTGAGGCCAATTGTGGTAAATTCGACACTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTGCCGTCGCGAGCGATAACGCAGCGCCCTATGCGGATGCCATTCGTTATAGTGTTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGG                                                                                 >  2:273910/1‑251                                                                                                                                                                         CCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTT                                                                               >  1:349190/1‑251                                                                                                                                                                             AATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGG                                                                           >  1:203725/1‑251                                                                                                                                                                                CGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCAT                                                                        <  1:78712/251‑1                                                                                                                                                                                                  GCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACG                                                      >  1:387563/1‑251                                                                                                                                                                                                                   AAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGGTGGATTTGCCCCGAGATTTCCAGACATCTGTTATAACTTAACCCATTACAAGCCCGCTGCAGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGGTGCCAGTCGTTATAATGCTTGAACGCCTCTGCAAGGTTCTGTGCTGCTGTTAACCCGTCTGGTTAGGGCATGATACTGATGTAGGCACGCTTTGTCGTTTTCGCGA                                     <  2:264495/251‑1                                                                                                                                                                                                                     AGGTGCTTTTGCCGTTTTCGGACTGCGGTCAATTGTGGAGGATTTGCACCTATATTTCCAGACATATGTTATCAATTCACCCATTACAAGCCCGCTGCCGCAGCTATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTAGAACGCCTCTGCAAGGTTATTTGCTGCCGTTCACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAA                                    >  2:264263/1‑250                                                                                                                                                                                                                                                    ATTGTGGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCC    <  2:79877/251‑1                                                                                                                                                                                                                                                       GTGGTGGATTTGCCCCTATATGTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCTGATGCCAGTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCAGGATACTGATGTAGTCACGCTTTATCGTTGTCACGAAGCTCTCTTCTATTCCGTTACTCGCCGGACTCCGC  <  2:362772/250‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  AAACAGCGCCTGACGCTCACGCTGGTTGGCGAACCACGCCATTTTACGGCTACGGGCTTTATCAAACATATGGCCAATTAACAGGCCTAACACTACGCCCCAAAAGCCGCCGCCCATCAGTAAGGCCACGGCCACGCCAATGATTTTTCCCCAATACTGCATATATTCCCCAAATCGACACACGGATATCAGGGCTATCTCCCACAATATAAAGGTGCTTTTACCGTTTTCCGGCTGCGGTCAATTGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/57525‑57276 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGC  <  NC_000913/382590‑382345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.