Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	199.8 / NA	55	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (27/28);  total (27/28)

CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560216‑3560698                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ccATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAgg                                                                                                                                                                                                                                           >  2:192937/1‑251 (MQ=255)  cATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGt                                                                                                                                                                                                                                          >  2:148780/1‑251 (MQ=255)    tCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                                        >  2:50989/1‑251 (MQ=255)           gtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCg                                                                                                                                                                                                                                 >  1:239014/1‑251 (MQ=255)              aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                               >  1:321358/1‑250 (MQ=255)               gCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                               >  2:134127/1‑249 (MQ=255)                     ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                       >  2:86167/1‑251 (MQ=255)                                ggTGACAATGCGCAAATTGCTGTGATTGAGCAGTTCGGGCGCTCCCGCTTCCGGCGTGGGGCCGATAT‑CGATAAACA‑GCTTCTAGCAATTGGGGATTT‑GCTCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                                                                                                                                            <  1:124732/251‑1 (MQ=255)                                ggTGAAAATGCGAAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATGGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                                                                                                                             <  1:134127/250‑1 (MQ=255)                                     caatgggaaaaTTGCTGTGATTGATCAGGGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGTTTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                                                                                                                       <  1:13252/243‑1 (MQ=255)                                        tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                                    >  1:70645/1‑235 (MQ=255)                                        ggcgcAAATTGCTGTGATGGAGAGGGGCGGGCGCTCCCGCTTCCGGGGGGTTGCCGATAC‑CGA‑AAACAAGCGTCGTGC‑ATTTGGGATTTTGTGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                                    <  2:70645/234‑1 (MQ=255)                                               aTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                             >  2:253889/1‑251 (MQ=255)                                                  gCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                                           >  2:410475/1‑250 (MQ=255)                                                      tgATTGAGCAGTGCTTGTGCTACGTTTTCGGGCGTGGTGC‑GATTTTCGAAAAACA‑GCGTCGACCAATTGGTGTTTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCGTCGTTCTGGGTG‑GCCTTGCGTTCGTGCCACGGCGTTTTATCCGAACTGGAAGGCAGCGCCGCACCGCTATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                       <  1:412222/250‑1 (MQ=255)                                                                 ggcgtgcgCTACCGCTTCCGGCGTGGGGCCGATAT‑CGATAACCA‑GCGTCGAGCCATTGGGGTTTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                                                                                                                                           <  1:258593/250‑1 (MQ=255)                                                                           cccgtctcgggCGTGGTCCGGATAT‑CGATAACCA‑GCGCGGAGCCTTGGGGGTTTT‑CCCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTG‑GCCTTGCGACCGTGCCCCGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                                 <  2:1922/242‑1 (MQ=255)                                                                            ccGCGTCCGGCGTGGTGCCGATAT‑CTAAAACCA‑GCGGCGAGCCTTTGGGGTTTT‑GCTCCGCCAGTTTGCGGGCGAGGCGCTTTGTTTCTGGGGTCGGGGTG‑TCCTTGGGATCGGGCCACGGCGTGTTAGCCGAGCTGGAAGGCAGCGCGGCTCCGCCATGATGGCGCAGGAGCAGGGTTTGGTCCGCCATCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                                                                                                                                <  1:272719/251‑1 (MQ=255)                                                                               tttCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGCATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                              >  2:118390/2‑250 (MQ=255)                                                                                            gCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                                >  1:367075/1‑251 (MQ=255)                                                                                             cctatat‑CGATAAACA‑GCGTCTAGCCATTGGGTATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                                                                                                                               <  1:279852/248‑1 (MQ=255)                                                                                                    atagaaaaacggcgtagcgCCATGGGGGTTTT‑CCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGG‑GCCTTGCGATCGTGCCGCGGGGGTTTACCCGAACTGGAAGGCACCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                                          <  2:140783/233‑1 (MQ=255)                                                                                                      gATAAACA‑GCGTCGGGCTATGGTGGTTTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTTTGGTTG‑GCCTTGCGATCGTGCCACGGCGGGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCGGGATCATGTTTTGCTCCGCCAGTTCATTGAGGTCGCGGCTAATAGTCTGCGGGCTGACGGAGATATTCTCGACCAGCTCTTCGGTACTGACATAACCCTTCTGTTTaa                                                                                                                                       <  1:205821/251‑1 (MQ=255)                                                                                                             a‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                                >  1:319592/1‑251 (MQ=255)                                                                                                                gcttcgggccgtGGGGATTTTGCCCCGCCACTTGGGGGGCGGGGGGCTCTTTTTCTTCGGTCTGGGTG‑GCCTGGCGATCGTGCCAGGGCGTGTTATCCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGCTCg                                                                                                                               <  2:349182/240‑1 (MQ=255)                                                                                                                 gTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat                                                                                                                             >  1:283866/1‑251 (MQ=255)                                                                                                                    gAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                          >  2:281241/1‑251 (MQ=255)                                                                                                                           tGGGGATTG‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                                    <  1:19549/250‑1 (MQ=255)                                                                                                                            ggggATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                                  >  1:383196/1‑251 (MQ=255)                                                                                                                              ggATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                                                 >  2:336625/1‑250 (MQ=255)                                                                                                                                     gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                           <  1:174373/250‑1 (MQ=255)                                                                                                                                        ccgccgCTTTGCGGGCGTGGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGGGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                                       <  2:337201/251‑1 (MQ=255)                                                                                                                                           ccccTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                    <  2:283866/248‑1 (MQ=255)                                                                                                                                           ccACTTGGCGGGCGATGCGCTTTTTTTCTTCGGTCGGGGTG‑GCCTTGCGATCGTGCCCGGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                    <  2:165063/251‑1 (MQ=255)                                                                                                                                                 ggCGGGGGATGCGCTTTTTTTCTGCGGTCTGGGGG‑GCCTTGCGATCGTGCCGCGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                              <  2:300451/250‑1 (MQ=255)                                                                                                                                                  gggggCGATGGGCTTGTTTTCTTCGGT‑TGGGGGTGCCTTGCGATCGGGCCGCGGGGTGTAAACCGAATGGGAAGCCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATTCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGGTCGATAATACCGTGGTGACGTTGTGTTTGTTTCAttt                                                                                             <  2:243655/249‑1 (MQ=255)                                                                                                                                                  gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                             <  1:281241/251‑1 (MQ=255)                                                                                                                                                        gATGCACTCTATTTCTTCGGTCCGGGAG‑GCCTTGCGATAGCGCCACGGCGTGTTATCCGCACTGGAAGGCAGCGCCGCCCAGCCCTGATGGCGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTATTCGGTACTGACCTAACCCTGCTGCTTAACCAGTTCGCTAATACCGATGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                                       >  2:36923/1‑251 (MQ=255)                                                                                                                                                                ctTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAACAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCATGGa                                                                                >  1:392510/1‑250 (MQ=255)                                                                                                                                                                  ttttCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                                             >  2:146539/1‑251 (MQ=255)                                                                                                                                                                    ttcgtcGGTCTGGGGG‑GCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatta                                                                            <  1:228326/246‑1 (MQ=255)                                                                                                                                                                            tCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttt                                                                   >  1:234360/1‑251 (MQ=255)                                                                                                                                                                               gggTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                         >  1:337080/1‑146 (MQ=255)                                                                                                                                                                               gggTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                         <  2:337080/146‑1 (MQ=255)                                                                                                                                                                                     gCCTTGCGACCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAt                                                           <  2:257323/251‑1 (MQ=255)                                                                                                                                                                                           cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                     >  1:283103/1‑251 (MQ=255)                                                                                                                                                                                             aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                                    >  1:283310/1‑250 (MQ=255)                                                                                                                                                                                                     aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                           <  2:234360/251‑1 (MQ=255)                                                                                                                                                                                                               aaCCGAAATGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc                                  >  2:351455/1‑250 (MQ=255)                                                                                                                                                                                                                                      cACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAt          <  1:402486/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAt                                                                                               <  2:50149/163‑1 (MQ=255)                                                                                                                                                                                                                                         cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAt                                                                                               >  1:50149/1‑163 (MQ=255)                                                                                                                                                                                                                                           ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAAGAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgtg     <  1:192937/251‑1 (MQ=255)                                                                                                                                                                                                                                           ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTACGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgtg     <  2:283310/251‑1 (MQ=255)                                                                                                                                                                                                                                              tgatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTc  >  1:24938/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATAT‑CGATAAACA‑GCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTG‑GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560216‑3560698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑