Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	149,091	C→T	E171K (GAA→AAA)	panB ←	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	149,091	0	C	T	100.0%	234.5 / NA	69	E171K (GAA→AAA)	panB	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/33);  total (36/33)

TCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTCTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGC  >  NC_000913/148853‑149331                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  tCAGCCATATCCTCCCGCACAGCCGCGCGGATGCCGCCCGTTTCGGCGAGAAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑t                                                                                                                                                                                                                                        <  1:527980/249‑1 (MQ=255)        tataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTg                                                                                                                                                                                                                               >  1:349159/1‑251 (MQ=255)        tataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTg                                                                                                                                                                                                                               >  2:205510/1‑251 (MQ=255)         ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTg                                                                                                                                                                                                                               >  1:73410/1‑250 (MQ=255)                     aGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGc                                                                                                                                                                                                                  >  1:277155/1‑251 (MQ=255)                        cgcgggGAGGTCCCCCGTTTGGGGGAGGAATTTTTTAGCGATTTGAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCg                                                                                                                                                                                                               <  1:437485/251‑1 (MQ=255)                          cgcgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCt                                                                                                                                                                                                             >  1:308372/1‑251 (MQ=255)                           gcgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCtt                                                                                                                                                                                                            >  1:264223/1‑251 (MQ=255)                            gggATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTc                                                                                                                                                                                                           <  1:267161/250‑1 (MQ=255)                             ggATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTTATCGCCCGCTTcc                                                                                                                                                                                                          >  2:419154/1‑250 (MQ=255)                                      ccGTTTGGGAGAGAAAATTTTTAGGGAATTGAGGATGGGGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCgcgc                                                                                                                                                                                                 <  2:233149/251‑1 (MQ=255)                                             ggCGAGGAAATTTTTCGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTAGTGCATCAAGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGa                                                                                                                                                                                           >  2:518785/1‑250 (MQ=255)                                                    aaaTTTTTAGCGAATTTAGGAATGTGACCGACGGAAATACCAACGGCGTCGTCCACCAAGAGAATCTGCCCGTCCGTGACGTTGCCTGCGCCAATGCCAATAACAGGGATAGCCAGAGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCACGCTTCATCGCCGCGCCCCTGAACTTTGt                                                                                                                                                                                    >  1:59362/1‑250 (MQ=255)                                                     aaTTTTTAGAGAATTAAGGAAGGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAg                                                                                                                                                                                  <  1:136231/251‑1 (MQ=255)                                                          ttacgaagttgggaaaTGTGACCGCCGTTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCAGCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTGGGAGCCAc                                                                                                                                                                              <  2:205134/236‑1 (MQ=255)                                                                 aTTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAat                                                                                                                                                                       >  2:56377/1‑250 (MQ=255)                                                                  tgtaggagggttaCCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCAGCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAata                                                                                                                                                                      <  2:236452/238‑1 (MQ=255)                                                                             gACCGCAGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACtgt                                                                                                                                                          >  2:480203/1‑251 (MQ=255)                                                                               ccgccgGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTgtgg                                                                                                                                                        >  2:213270/1‑251 (MQ=255)                                                                                 gccgGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTgtggtg                                                                                                                                                      >  2:35353/1‑251 (MQ=255)                                                                                   cgGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGtt                                                                                                                                                    >  1:396790/1‑251 (MQ=255)                                                                                       aaTACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTaaa                                                                                                                                                 <  1:99719/250‑1 (MQ=255)                                                                                        aTACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAAcc                                                                                                                                               >  1:212367/1‑251 (MQ=255)                                                                                          aCCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTa                                                                                                                                             <  1:351975/251‑1 (MQ=255)                                                                                             aaaGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGt                                                                                                                                          >  1:214880/1‑251 (MQ=255)                                                                                                ggCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGAc                                                                                                                                       >  2:201587/1‑251 (MQ=255)                                                                                                  cgtcgtGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACttt                                                                                                                                                                                      <  2:483982/202‑1 (MQ=255)                                                                                                  cgtcgtGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACttt                                                                                                                                                                                      >  1:483982/1‑202 (MQ=255)                                                                                                     cgtGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACAt                                                                                                                                  <  1:480203/251‑1 (MQ=255)                                                                                                       tGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATAc                                                                                                                                >  2:70588/1‑251 (MQ=255)                                                                                                            cACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGaa                                                                                                                           <  1:302873/251‑1 (MQ=255)                                                                                                              cGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATTCCAATAACCGGGATCGCCAGTGCTTCGGTAATTCGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGGTGATCGCCCGCTTCATCGCTGCGCCCCTGAACGTTGTAGCCACTGAAAATATTCACTGAGTGTTGTGTTAAACCTAAGGGACCACATAGATGAACg                                                                                                                         <  2:517332/251‑1 (MQ=255)                                                                                                               gAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACgg                                                                                                                        <  2:186389/251‑1 (MQ=255)                                                                                                               gAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTCCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGTGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACgg                                                                                                                        <  1:454148/251‑1 (MQ=255)                                                                                                                       gCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCg                                                                                                                <  2:232435/251‑1 (MQ=255)                                                                                                                                   cGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGt                                                                                                    <  1:117508/251‑1 (MQ=255)                                                                                                                                      tGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAg                                                                                                                              <  2:277149/222‑1 (MQ=255)                                                                                                                                      tGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAg                                                                                                                              >  1:277149/1‑222 (MQ=255)                                                                                                                                         cTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGtt                                                                                              >  1:16503/1‑251 (MQ=255)                                                                                                                                           gcgcCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGttt                                                                                             <  2:73410/250‑1 (MQ=255)                                                                                                                                              ccaatgccaatAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTTTACGGTTTCTAc                                                                                         <  2:308372/251‑1 (MQ=255)                                                                                                                                                    ccaatAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCa                                                                                   <  1:205510/251‑1 (MQ=255)                                                                                                                                                             gggATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCAc                                                                                                                                                                              >  1:41115/1‑151 (MQ=255)                                                                                                                                                             gggATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCGGAACTTTGTAGCCAc                                                                                                                                                                              <  2:41115/151‑1 (MQ=255)                                                                                                                                                             gggATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACAAGAACACATTCGAGAACCAGCAGCAGTGCCCAAACAGCTATAAAGGCTAA‑GGGATCGCTGAGAAGTTGATCGCCCGCTTCAAGACCGGGACACTGAACTTTGTAGCCACCGAAAATATTGAAGGAATGGGGTGGTAAACGAAAGTGAAAACATACAGGAACGGCACGTTCGGTCAGCATTTGTACggaatatagcagacagtcacc                                                                             >  1:86212/1‑229 (MQ=255)                                                                                                                                                                 tCGCCAGTGCTTCGGTACTCCGTTTTGCCAGTCCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCCGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGACACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGc                                                                                                          >  2:521383/1‑215 (MQ=255)                                                                                                                                                                 tCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATGCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCGCTGAACTTTGTAGCCGCCGAAAATATTCACTGACTGTGGGGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGc                                                                                                          <  1:521383/215‑1 (MQ=255)                                                                                                                                                                  cGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCa                                                                      <  1:207083/250‑1 (MQ=255)                                                                                                                                                                     cAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCAcc                                                                             >  2:193386/1‑240 (MQ=255)                                                                                                                                                                     cAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCAcc                                                                             <  1:193386/240‑1 (MQ=255)                                                                                                                                                                     cAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCACttt                                                                  >  1:317292/1‑251 (MQ=255)                                                                                                                                                                          cTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGAcc                                                             >  1:382598/1‑251 (MQ=255)                                                                                                                                                                               gtaatacgataagCCACTTCAACCGGCCTGCATTCCGGCACCAGCAGCTGTGCCACAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTCGCCACCGACAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGCACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGtt                                                        >  2:326637/13‑251 (MQ=255)                                                                                                                                                                                           gCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACg                                            >  2:142595/1‑251 (MQ=255)                                                                                                                                                                                                  cAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAAc                                      <  1:424324/250‑1 (MQ=255)                                                                                                                                                                                                   aaCCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTCCCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGTCACGCATAACCg                                    <  1:135516/251‑1 (MQ=255)                                                                                                                                                                                                        gCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTgcg                               >  1:215752/1‑251 (MQ=255)                                                                                                                                                                                                            gcattaaagcaccagcgGCTGCGCACTAGCAGATTTTAAGGCTAA‑TGCATCGCTGAGAAGTTGATCACACGCTTCATCGCTGCGCCCCTGAAATTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGACACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGt                           >  2:499105/8‑251 (MQ=255)                                                                                                                                                                                                             cATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGTCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGtt                          <  2:486497/251‑1 (MQ=255)                                                                                                                                                                                                              aTTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGttt                         >  1:140534/1‑251 (MQ=255)                                                                                                                                                                                                                 ccagcccccagagcTGTGGCCCAGCAGCTTTTAAGGTTAAGTG‑ATCGCTGAGAAGTTGATCGCCCGCTTCATCGCCGGGCGCCTGAACTTTGTAGCCACCGAACATATTCACTGACTGGGGTGTTAAACCTAAGTGACCACATTCAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGtggaggaggggtag                      <  1:252671/240‑14 (MQ=255)                                                                                                                                                                                                                  cagcaccagcaGCTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCg                      >  2:51558/1‑250 (MQ=255)                                                                                                                                                                                                                     ccccagcaGTTGTTCCCCAGCAGCTTTTAAGGCTAA‑TGCCTCGCTGAGCAGTTGATCGCCCGCTTCCTCGCCGCGCCCCTGACCTTTGTAGCCACCGAAAATATTCCCTGTCTGTGTTGTTAAACCTAAGTTACCACATACAGGAACGGCCCGTTCGGTCAGCATTTGTCCGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATATCCGTTGCGGCGTTTTCGAAgg                  <  2:468954/249‑1 (MQ=255)                                                                                                                                                                                                                           cagcTGTGCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGtt            >  2:416575/1‑251 (MQ=255)                                                                                                                                                                                                                               tgtgCCCCAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCgg        >  2:15641/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccccAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCAGAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTg    <  1:28430/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCa                                                                                                                      >  1:27441/1‑135 (MQ=255)                                                                                                                                                                                                                                     ccAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCa                                                                                                                      <  2:27441/135‑1 (MQ=255)                                                                                                                                                                                                                                     ccAGCAGCTTTTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGc  <  1:56377/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  TCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCCAATGCCAATAACCGGGATCGCCAGTGCTTCGGTAATACGTTTTGCCAGTTCAACCGGCACGCATTCCAGCACCAGCAGCTGTGCCCCAGCAGCTTCTAAGGCTAA‑TGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGC  >  NC_000913/148853‑149331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑