Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,236,311	C→T	S17L (TCA→TTA)	yjbF →	lipoprotein YjbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,236,311	0	C	T	100.0%	178.3 / NA	52	S17L (TCA→TTA)	yjbF	lipoprotein YjbF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/19);  total (33/19)

TCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAA  >  NC_000913/4236017‑4236602                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     tCGGCGGGGGGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCTGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTACGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGATAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCa                                                                                                                                                                                                                                                                                                 <  1:296362/300‑1 (MQ=255)          aGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAag                                                                                                                                                                                                                                                                                        <  2:875408/300‑1 (MQ=255)            ttCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAagag                                                                                                                                                                                                                                                                                      <  1:17235/300‑1 (MQ=255)                         aGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGTGACCTGCACTCATTCTTATCTGCATGTTATTACAGGCATATTTAGCCACGACTAAAGAGCTGGGAAACTCAc                                                                                                                                                                                                                                                                         >  2:170895/1‑300 (MQ=255)                           cGTCAGCACCGTACGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCAAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACCCATTCTTACCTGCATGTTATTACAGGCCTGTTTAGCCACGAATAAAGAGCTGGGCAATTCACtg                                                                                                                                                                                                                                                                       >  2:757817/1‑300 (MQ=255)                           cGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACtg                                                                                                                                                                                                                                                                       >  1:782345/1‑300 (MQ=255)                           cGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGAACTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACtg                                                                                                                                                                                                                                                                       >  1:133364/1‑300 (MQ=255)                                 cACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGAc                                                                                                                                                                                                                                                                 <  1:365743/300‑1 (MQ=255)                                   ccGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCCCTGTGGGACAg                                                                                                                                                                                                                                                               >  1:892152/1‑300 (MQ=255)                                   ccGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCAAGAACAACAGTACCCAGTAATAAAGTATGTATCCCCAAAATCATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAAATACACCTCGGTGTGGTTATTCTGACCCTCTGGAGAAGAGTCGTGAAGCTACCCGCACTCACTCTTCTCTACCCGCTATCACAGGCCTGTTTAGCCACCACTAAAGAGGTGGGCactcccccatgggacaa                                                                                                                                                                                                                                                               >  2:78330/1‑284 (MQ=255)                                                    ggtgTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAATCTGTTCGGCACGCCAg                                                                                                                                                                                                                                              >  2:35747/1‑300 (MQ=255)                                                    ggtgTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGAACTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAg                                                                                                                                                                                                                                              >  1:765319/1‑300 (MQ=255)                                                           cgcTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACATTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGTGACCTGCACTCATTCTTCTTTGCCTGTTATTACAGGCCTGGTTAGCCAAAACTAAAGAGATGGGTAATTCACTGTGAGACAGTCCGTTCGGCACAACAGGCGTACa                                                                                                                                                                                                                                       >  2:271553/1‑300 (MQ=255)                                                                                      gTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCCGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCCCTGTGGGAAAGTCTGTTCGGCACGCAAGGCGTCCAGCTGACGGATGATGATATTCAAAatat                                                                                                                                                                                                            >  1:696773/1‑300 (MQ=255)                                                                                      gTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGAACTGCACTCATTCTTATCTGCCTGTTATTCCAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCCCTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTAACGGATGATGATATTCAAAatat                                                                                                                                                                                                            >  2:377280/1‑300 (MQ=255)                                                                                           aaTACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCCGTTATTACAGGCCTGTTTCGCCACGACTAAAGAGATGGGAAATTCCCTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATCTTCAAAATATGCCCt                                                                                                                                                                                                       >  1:754643/1‑300 (MQ=255)                                                                                              aCCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTCGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACCGCTGACGGATGATGATATTCAAAATATGCCCTACg                                                                                                                                                                                                    >  1:412241/1‑300 (MQ=255)                                                                                                          acaACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCCAAATATGCCCTACGCCAGCCCGTACa                                                                                                                                                                                        >  1:571090/1‑300 (MQ=255)                                                                                                                   accaccAGGACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGTAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCtga                                                                                                                                                                              <  1:347672/300‑3 (MQ=255)                                                                                                                    ccaccaGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCCCTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTaa                                                                                                                                                                             >  1:350309/1‑300 (MQ=255)                                                                                                                     caccaGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTAACGGATGATGATATTCAAAATATGCCCTCCGCCAGCCAGTACATG‑CAGCTTAAt                                                                                                                                                                            >  1:483945/1‑300 (MQ=255)                                                                                                                     caccaGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGAACTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGTTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATCTTCAAAATATGCCCTACGCCAGCCAGTCCATG‑CAGATTAAt                                                                                                                                                                            >  1:270894/1‑300 (MQ=255)                                                                                                                       ccaGTACCAAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTTATTCTTATCTGCCTGTTATTACAGGCCTGTTTCGCCCCGACTAAAGCGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCCGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATgg                                                                                                                                                                          >  2:524911/1‑300 (MQ=255)                                                                                                                                 gTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAg                                                                                                                                                                >  1:899311/1‑300 (MQ=255)                                                                                                                                                                               ggCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATggg                                                                                                                  >  1:492637/1‑300 (MQ=255)                                                                                                                                                                               ggCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGAACTGCGCTTATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCAAGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCNGCACGCCAGGTGTACAGCTGAAGGATGATGATGCTCAAAATCTGCCCTACGCCAGCCAGTACATG‑CCGTTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATggg                                                                                                                  >  2:210630/1‑300 (MQ=255)                                                                                                                                                                                       gTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAg                                                                                                          >  1:760622/1‑300 (MQ=255)                                                                                                                                                                                          tGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTNAGCCACGACTAAAGAGCTGGGCAATTCACTGTTGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGAt                                                                                                       <  1:662961/300‑1 (MQ=255)                                                                                                                                                                                           gAAGGGATTAACCATAACCACACTCAGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTAAGCCAGCCAGTACCTG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATc                                                                                                      >  1:475656/1‑300 (MQ=255)                                                                                                                                                                                               ggATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACTTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTTGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGc                                                                                                  <  2:388320/300‑1 (MQ=255)                                                                                                                                                                                               ggATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGc                                                                                                  <  1:544916/300‑1 (MQ=255)                                                                                                                                                                                                gATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCt                                                                                                 >  1:886973/1‑300 (MQ=255)                                                                                                                                                                                                    aaccataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTActc                                                                                             <  1:886672/300‑1 (MQ=255)                                                                                                                                                                                                     accataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTTGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTActct                                                                                            <  1:867277/300‑1 (MQ=255)                                                                                                                                                                                                     accataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTActct                                                                                            <  1:748898/300‑1 (MQ=255)                                                                                                                                                                                                     accataaccaCACTCCGGTGTAGTTATTCTGCCCCTCTGTAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTTGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTActct                                                                                            <  2:151156/300‑1 (MQ=255)                                                                                                                                                                                                         taaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACNGTGGGACAGTCTGTTCGGCACGCCAGNCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGtt                                                                                        >  2:619056/1‑300 (MQ=255)                                                                                                                                                                                                         taaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGAACTCATTCTTCTCTGCCTGTTATTACAGGCCTGTTTCTCCACGACTAAAGAGCTGGGCAATTCACCGTGGGACAATCTGTTCGGCACGCCAGGCGTACAGCTGAAGGATGATGATATTTCAAATATGCCCTCCGCCAGCCAGTACATGTCA‑CTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAAAAAAAATGGGTCGCTCAGGATCAGGTTGCTCTCGtt                                                                                        >  1:329027/1‑300 (MQ=255)                                                                                                                                                                                                            ccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGNCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTAcc                                                                                     >  1:637783/1‑300 (MQ=255)                                                                                                                                                                                                             cacaCTCCGTTGTGGTTATTCTGCCCCTCTGTAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTAccc                                                                                    <  2:495591/300‑1 (MQ=255)                                                                                                                                                                                                                     ggTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGc                                                                            <  1:521082/300‑1 (MQ=255)                                                                                                                                                                                                                         tgGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTc                                                                        >  1:411815/1‑300 (MQ=255)                                                                                                                                                                                                                              aTTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAA‑TTGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGa                                                                  >  1:867233/1‑300 (MQ=255)                                                                                                                                                                                                                                   gCCCCTCTGGATAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCGGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGAc                                                              <  1:383807/300‑1 (MQ=255)                                                                                                                                                                                                                                   gCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTTGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCGTAATGGCGGGCCGCAGTTAGTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGAc                                                              <  1:59977/300‑1 (MQ=255)                                                                                                                                                                                                                                      ccTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGCAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGActct                                                           >  1:520312/1‑300 (MQ=255)                                                                                                                                                                                                                                            gagaAGAGTCGTGAAGCGACCTGCACTCACTCCTATCTGCCTGTTATTACAGGCCTGTTTAGCCAAGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTAAGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGAAAGACAACAAAAATGGGTCACTCAGGGTCAGGCTACTCTCGTTACCCAAAATGGACGTCTGGTGAAGACTCTGCTcgg                                                     >  2:231235/1‑300 (MQ=255)                                                                                                                                                                                                                                             agaagaGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAGAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATTGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTcggc                                                    <  2:312724/300‑1 (MQ=255)                                                                                                                                                                                                                                             agaagaGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTcggc                                                    <  2:809311/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTg                                    >  1:138592/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        atccTTATCTGCCTGTTATTACAGGCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTTTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTGCTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAAc                         <  2:130531/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tGTTATTACAGGCCTGTTTAGCCACTACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGAcc            <  2:799992/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               ggCCTGTTTAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGTACGCCAGGCGTACAGCTGACGGATGGTGATATTCAAAATATGCCCTACGTTAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCGGTTATTTGTGGTGCTGGCCTTCGCTGACGACGGACAACAAAAATGGGTCACTCAGGCTCGGGCTACTCTCGTTACCCAGCATGGCTGTTTGGTGAAGGCTTTGTTTGGCGGCGACACCCTGATTGACGTGAATAACATTGCTGCCGACATGCTGATTaa  >  2:30063/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     TCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATG‑CAGCTTAATGGCGGGCCGCAGTTATTTGTGGTGCTGGCCTTCGCTGAAGACGGACAACAAAAATGGGTCACTCAGGATCAGGCTACTCTCGTTACCCAACATGGCCGTCTGGTGAAGACTCTGCTCGGCGGCGACAACCTGATTGAAGTGAATAACCTTGCTGCCGACCCGCTGATTAA  >  NC_000913/4236017‑4236602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑