New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1287329	65 (0.640)	9 (0.130)	8/424	5.7	18.0%	intergenic (‑1/+209)	tyrV/tyrT	tRNA‑Tyr/tRNA‑Tyr
	?	NC_000913	1287624 =	36 (0.500)					intergenic (‑2/+158)	tyrT/purU	tRNA‑Tyr/formyltetrahydrofolate deformylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTA  >  NC_000913/1287325‑1287908                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             caccaGGGGTAATGCAAATTTTGAGGTAATACTTGAGATGGTGGTGGGGGAAGGATTATGCGTCGCTTCGCTCCTCACCCTGCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCACTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCcaccac                                                                                                                                                                                                                                                                                              <  1:259812/300‑1 (MQ=255) caacaGGGGTAATCCCAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTAGGGCCGTTGCCTGTGGCCACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTACCCGCTGAGTGCAAACTTTCACAATCTCAACGAAGTTACCAAATCGCTGTGGTGAATTCTGGTGGGGGGAGAAGGATTCGAACCTTCGAAGTCGACGCAGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCcaccac                                                                                                                                                                                                                                                                                              >  2:283866/4‑300 (MQ=38)      ttggTAATTCAACTTTTTAGTTAATGCTTGAGATGGTGGTGGTGGAAGGATTATTCGTCGCTTCGCTCCCCACCCGTCGGGCCGGTGCCCTCGGAAACGTTCTCTCGCTGCAGCGCGAAGAGAACCTTAGGCGAAGGTTATCACCCTTCTCCTATGAGTGCAAACTTTCGCAATCTTACTTAGGTCACTACATCGCTTTGGGGAATGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGt                                                                                                                                                                                                                                                                                         <  1:160849/298‑1 (MQ=16)                 attccggggcttttgaaagtgaTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGNGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGCTGACGGCAGATTTACCGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTa                                                                                                                                                                                                                                                                              >  1:580378/21‑300 (MQ=25)                            aaTGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCTTTACTCTTAGGGTTGTGGCCTGGGGCGACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTc                                                                                                                                                                                                                                                                   <  1:725292/300‑1 (MQ=38)                              tGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTccc                                                                                                                                                                                                                                                                 >  1:416314/1‑300 (MQ=255)                                        ggtggtggGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGaa                                                                                                                                                                                                                                                       >  1:187673/1‑300 (MQ=255)                                         gtggtggGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAg                                                                                                                                                                                                                                                      >  1:320319/1‑300 (MQ=255)                                              gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACNACATCGCTGTGGTGAATTATGGTGGTNGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgggg                                                                                                                                                                                                                                                 <  2:567224/300‑1 (MQ=38)                                                    aGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGcatc                                                                                                                                                                                                                                           >  1:285592/1‑300 (MQ=255)                                                     ggATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGcatca                                                                                                                                                                                                                                          >  1:677789/1‑300 (MQ=255)                                                                       ctcctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGcgccgc                                                                                                                                                                                                                        >  1:244992/1‑300 (MQ=255)                                                                         cctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTg                                                                                                                                                                                                                      >  1:387483/1‑300 (MQ=255)                                                                           tcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAGTGCTTTTTCCTGGCATGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTa                                                                                                                                                                                                                    >  2:235476/1‑300 (MQ=255)                                                                              cccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCAACCTTCCCCGATGCGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTGTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTAATGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGtcgag                                                                                                                                                                                                                 >  1:246783/1‑296 (MQ=255)                                                                                cTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgtg                                                                                                                                                                                                               >  1:172490/1‑300 (MQ=255)                                                                                      gCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGt                                                                                                                                                                                                         <  1:170654/300‑1 (MQ=255)                                                                                      gCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCACTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTTTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGt                                                                                                                                                                                                         <  1:152181/300‑1 (MQ=255)                                                                                                                           aatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTa                                                                                                                                                                    >  1:617708/1‑300 (MQ=255)                                                                                                                                       gTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAgg                                                                                                                                                        <  1:437952/300‑1 (MQ=255)                                                                                                                                       gTCGAAGGGTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAgg                                                                                                                                                        <  1:437939/300‑1 (MQ=255)                                                                                                                                        tCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTCCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGTCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGt                                                                                                                                                       <  2:305322/300‑1 (MQ=255)                                                                                                                                        tCGAAGGTTCTCACCCTTCCCATAGGAGTGCAAACTTTCACAAGCTCACCTAAGGTACCACATCACTGTGGTGAATGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGt                                                                                                                                                       <  2:697833/300‑1 (MQ=38)                                                                                                                                         cGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTNCGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTNAAGGTa                                                                                                                                                      >  2:681542/1‑300 (MQ=255)                                                                                                                                                     cccTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTNACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTNCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAtttt                                                                                                                                          <  2:662397/300‑1 (MQ=255)                                                                                                                                                         tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATTGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAAGTAAACAGTTATTTTTGCt                                                                                                                                      >  2:376105/1‑300 (MQ=255)                                                                                                                                                         tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCt                                                                                                                                      >  2:402278/1‑300 (MQ=255)                                                                                                                                                         tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCt                                                                                                                                      >  1:98862/1‑300 (MQ=255)                                                                                                                                                         tCCCCGATGAGTGCAAACTTTCACAATCTCAACGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCACTTTGGCCGC‑TCGGGAACCCCACCAAGGGGTAATGCTTTCTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATCTCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGCAAACTGACCTGGTTGCGTAATTTTCATCCGTACCGGATTAAAAGTAACCAGTTATTTTTGCt                                                                                                                                      >  2:74454/1‑300 (MQ=38)                                                                                                                                                                   gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTaca                                                                                                                            >  1:305982/1‑298 (MQ=255)                                                                                                                                                                   gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTaaa                                                                                                                            >  1:203013/1‑300 (MQ=255)                                                                                                                                                                                           aaGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAGTGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCAGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa                                                                                                    >  2:246444/1‑300 (MQ=255)                                                                                                                                                                                                             ggtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCa                                                                                  >  1:241262/1‑300 (MQ=255)                                                                                                                                                                                                                ggaTTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCATTTGGCCGC‑TCGGGAACTCCACCCAGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATTGGGAAGCGGGGCGCATCATATCCAATGCCGCGCCGCTGTAAAGTGTTAAGTTGAGAAAAATGAACTGGTTGCGGCATTTTCCTCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGTGATTCAAGAATAATCGTTCGCTTCCCGTACACAAGGGCGCGTTGTGCTAGTa                                                                               >  2:89848/3‑300 (MQ=25)                                                                                                                                                                                                                 aaTTATGGTGGTGGGGTAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAc                                                                              <  1:456709/300‑1 (MQ=255)                                                                                                                                                                                                                 aaTTATGGTGGGGGGGGGAGGATTTAGACCCTCGGAGTCGGGGAAGGCAGATTTGAAGGCCGCCCCCCTTTGCCGCCTCGG‑AACCCCACCACGGGGTAATTCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAc                                                                              <  2:381169/300‑1 (MQ=255)                                                                                                                                                                                                                     aTGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTg                                                                          >  1:361753/1‑300 (MQ=255)                                                                                                                                                                                                                        gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGtat                                                                       <  1:221731/300‑1 (MQ=255)                                                                                                                                                                                                                          ggttggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTGCAGTCTGCTCCCTTTTGCCGC‑TCGGGAACCCCACCACGGGTTAATGCTTTTTACTGGCCTGCTCCCTTATCTGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                                                     <  2:489238/296‑1 (MQ=255)                                                                                                                                                                                                                          ggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTNCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                                                     <  1:585563/300‑1 (MQ=255)                                                                                                                                                                                                                          ggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                                                     <  1:652052/300‑1 (MQ=255)                                                                                                                                                                                                                                  aaGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGNCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGAc                                                             <  2:620980/300‑1 (MQ=255)                                                                                                                                                                                                                                  aaGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGAc                                                             <  1:666417/300‑1 (MQ=255)                                                                                                                                                                                                                                  aaGGATTCGAACCTTCGAAGTCGATGACGGCAGAGCTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTCTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCACCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAACGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACGTTGTATAGTGCACGAc                                                             <  2:408815/300‑1 (MQ=255)                                                                                                                                                                                                                                    ggATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACtt                                                           >  1:381667/1‑300 (MQ=255)                                                                                                                                                                                                                                    ggATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACtt                                                           >  2:133622/1‑300 (MQ=255)                                                                                                                                                                                                                                         cGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGAc                                                      <  1:206147/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  gATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg                             >  1:63565/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCAACACGGGGTAGTGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACNGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGAAGTTTTTCTCGACGTCAAGAACTgcg                             >  2:206147/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             tGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATc                  <  1:314136/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGTGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAAAGGATTAAAGGTAAACAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGTATCATATCTTCa              >  1:699624/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAANAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCa              >  1:587436/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATACTCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACCTAAGACGTTTTTCTCGACGTCACGACCCGCGCGCATCATATCTTCa              >  2:255625/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCa              >  1:203391/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGAACTGCGCGCATCATATCTTCa              >  2:155524/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACACGCCACTGTAAAGTGTCACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCAGTAAAGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGCAGTCTTTCTCGACGTCACGACCTGCGCGCATCCTATCTTcc              >  1:48002/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                  ccTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg             >  1:4524/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         ccGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAAACAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGAATTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTAAGCTGTGTa      >  2:29223/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            c‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGt   >  1:780859/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              tCGGGANCCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGANGCGGTGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTa  <  2:684099/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tCGGGAACCCCACCACGTGGTAATGCTTTTTACTTGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTa  <  2:111114/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tCGGGAACCCCACCACGGGTTAATGCTTTTTACTGGCCTGCTCCCTTAGCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGATCTGGTTGCGTAATTTTCATCCGTAACGGATTAAANGTAACCAGTTATTTTTGCTGGCGATTNAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTa  <  2:541520/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             CACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGC‑TCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTA  >  NC_000913/1287325‑1287908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.