Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,435	G→A	S183F (TCT→TTT)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,435	0	G	A	97.8%	163.1 / NA	46	S183F (TCT→TTT)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (32/13);  total (33/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGACGCGATCGGCAAACGTGGCGTCGATCAATTTTTTCGCCAGT  >  NC_000913/1831145‑1831728                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                      tgCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTNTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCaa                                                                                                                                                                                                                                                                                              >  1:546041/1‑300 (MQ=255)  gccctGAGTGCCAACGGTCATCACGCGGACGCACTCTTCGGTTGTCAACAACGCACCGACCGGGAAACTGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCAGGTTTGTACTTCATCAAAAATCAACCGCGCATTGTGGCGGTTACACAACTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACAACACCGCACTCCCCATGGATGGGGTCGAAAATCACAGCATAGGTAAAGTCGTCAAt                                                                                                                                                                                                                                                                                             >  2:143247/5‑300 (MQ=255)  gCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACCCTCACCGCACAGGTAGAGTCGTCAAt                                                                                                                                                                                                                                                                                             >  2:370623/1‑300 (MQ=255)         gTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAgcgcg                                                                                                                                                                                                                                                                                      >  1:455614/1‑300 (MQ=255)                    catcaCGCGGGCGCACTCTTAGGTTGCAAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGtt                                                                                                                                                                                                                                                                           >  1:171927/1‑300 (MQ=255)                             ggCGCACTCTTCGGTTGCCAACAACGCACCGNCCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGNCGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCCCCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGAACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGtt                                                                                                                                                                                                                                                                  >  2:575402/1‑300 (MQ=255)                             ggCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGtt                                                                                                                                                                                                                                                                  >  1:697297/1‑300 (MQ=255)                                   ctctTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCNGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATNACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATgc                                                                                                                                                                                                                                                            <  2:730315/300‑1 (MQ=255)                                     ctTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTNTGTACTTCATCAAAAATCAACAGCGCNTTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATgcag                                                                                                                                                                                                                                                          >  2:531160/1‑300 (MQ=255)                                             gCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGa                                                                                                                                                                                                                                                  <  2:257066/300‑1 (MQ=255)                                                 acaacGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAatat                                                                                                                                                                                                                                              >  1:594864/1‑300 (MQ=255)                                                     cGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGGCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCAAGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCACCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATccgc                                                                                                                                                                                                                                          >  2:501121/1‑300 (MQ=255)                                                         ccgaccgGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGc                                                                                                                                                                                                                                      <  2:759041/300‑1 (MQ=255)                                                                ggAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGgcg                                                                                                                                                                                                                               >  1:305218/1‑300 (MQ=255)                                                                                    ttGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTNCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggct                                                                                                                                                                                                           <  2:584589/300‑1 (MQ=255)                                                                                          gTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGAATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGc                                                                                                                                                                                                     >  2:760134/1‑300 (MQ=255)                                                                                               tAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCAcc                                                                                                                                                                                                <  1:106724/300‑1 (MQ=255)                                                                                                    gATCAGNCGTCACGCCTTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGANGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCAc                                                                                                                                                                                           <  2:666873/300‑1 (MQ=255)                                                                                                    gATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCAc                                                                                                                                                                                           >  1:751971/1‑300 (MQ=255)                                                                                                      tCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTg                                                                                                                                                                                         <  1:218471/300‑1 (MQ=255)                                                                                                               aCGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCTCGACGCCGGTTTGTACGTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                                                                                                <  2:495655/300‑1 (MQ=255)                                                                                                                       gTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCCGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCCAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCg                                                                                                                                                                        >  1:738893/1‑300 (MQ=255)                                                                                                                         gCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                                                                                                      >  2:494601/1‑300 (MQ=255)                                                                                                                         gCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACAAATATCCGCAGGCAGTGGCGCAAAATCCTGTGAATAGGATGGCTGCACACCCGCACTGACAGTAAACAGCGCGCGAc                                                                                                                                                                      >  2:333361/1‑300 (MQ=255)                                                                                                                              taGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCCGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCACCTGGATGGGTTCGACAATCACCGCACCGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCCCTGACAGTAAACAGCGTGCGACCATGa                                                                                                                                                                 >  1:613716/1‑300 (MQ=255)                                                                                                                                ggCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaaa                                                                                                                                                               >  1:692345/1‑300 (MQ=255)                                                                                                                                   aTACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGc                                                                                                                                                            >  1:406296/1‑300 (MQ=255)                                                                                                                                        gTTCCCCGGTGCGCCCGACGCCGGTTTGTATTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtttt                                                                                                                                                       >  1:299834/1‑300 (MQ=255)                                                                                                                                        gTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtttt                                                                                                                                                       >  1:158565/1‑300 (MQ=255)                                                                                                                                                                tttGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                                                                                               >  1:6755/1‑300 (MQ=255)                                                                                                                                                                   gTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGc                                                                                                                            <  1:220528/300‑1 (MQ=255)                                                                                                                                                                    tACTTCATCAAAAAGCAACAGCGCATCGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCt                                                                                                                           <  2:35695/300‑1 (MQ=255)                                                                                                                                                                              aaaaTCAACAGCGCATTGTGGCGGTTGCACAATTCACGCAGACCTTTTAAAACCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTTGATGGGTTCGACAATCACCGCACGGTTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAAGATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTTGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATTAAACGCATTTTTGAGCGCCACGATGCCGCTCTTATGGCTGCCGTAGCgg                                                                                                                 <  2:278337/300‑1 (MQ=255)                                                                                                                                                                                                                 aCGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCANTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGcgccgc                                                                              >  1:540453/1‑300 (MQ=255)                                                                                                                                                                                                                        ccTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTg                                                                       >  1:465310/1‑300 (MQ=255)                                                                                                                                                                                                                            gTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGACACGCATTTTTCAACGCCACGATGCCGCTCTTATGGCTGNCGTAGCGGTCGTGAGCGAATTTACGCGCAAGTTTTAGCGCCGCTTCGTTGGCtt                                                                   >  2:586675/1‑300 (MQ=255)                                                                                                                                                                                                                                            ggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCCCCGGAGTTACa                                                   >  1:370572/1‑300 (MQ=255)                                                                                                                                                                                                                                             gctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACaa                                                  <  1:394477/300‑1 (MQ=255)                                                                                                                                                                                                                                                ggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAaag                                               <  1:40655/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAaaga                                              >  1:261202/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccccTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGCCGCGATCGGCaaa                              >  1:115630/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccccTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGACGCGATCGGCaaa                              >  2:777309/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccccTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGACGCGATCGGCaaa                              >  1:115156/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccccTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGACGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCCCCGGAGTTACAAACGAAGACGCGATCGGCaaa                              >  2:295326/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cccTGGATGGGTTCGACAATCACCGCACAGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGACGCGATCGGCAAAc                             >  1:348243/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             caGGTAAAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGACGCGATCGGCAAACGTGGCGTCGATCAATTTTTTCGCCAGt  >  2:493306/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                      TGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTCGTTGGCTTCCGCACCGGAGTTACAAAAGAAGACGCGATCGGCAAACGTGGCGTCGATCAATTTTTTCGCCAGT  >  NC_000913/1831145‑1831728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑