Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,075,684	C→T	A1067T (GCC→ACC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,075,684	0	C	T	100.0%	202.2 / NA	58	A1067T (GCC→ACC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/21);  total (37/21)

TCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCG  >  NC_000913/1075389‑1075975                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    tCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAg                                                                                                                                                                                                                                                                                                 >  1:232101/1‑300 (MQ=255)        atTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGa                                                                                                                                                                                                                                                                                          <  2:164970/300‑1 (MQ=255)             gTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTgg                                                                                                                                                                                                                                                                                     >  1:770707/1‑300 (MQ=255)                           cagttCTGGACGATTTGCTGCTCATCCCCGCAGTGCATTTAGCGGCTGAGTCAATGCTGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACGGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTtcatc                                                                                                                                                                                                                                                                       <  2:158680/297‑1 (MQ=255)                               tCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACCTACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTAATCAATg                                                                                                                                                                                                                                                                   >  2:772182/1‑300 (MQ=255)                                              gcCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCa                                                                                                                                                                                                                                                    >  2:370672/1‑300 (MQ=255)                                                             gCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACATGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATaa                                                                                                                                                                                                                                     >  1:636070/1‑300 (MQ=255)                                                             gCATTTAGCGGCTGAGTCAATGCGGCTTTCAAATGCGCATCGACCGGATACTTTGCCTCCTGACGCGCGAGCGTCAATGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGAACGCCTGCTTTCGGCCCGGTACCGGCCAACCCTTCGCAGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTCACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTAATCAATGCGCGTATGGACGCAAAGCGTCAGACCATaa                                                                                                                                                                                                                                     >  2:91885/1‑300 (MQ=255)                                                              cgtttAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAAc                                                                                                                                                                                                                                    <  2:242157/298‑1 (MQ=255)                                                              cATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGNATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGTTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAAc                                                                                                                                                                                                                                    <  2:604313/300‑1 (MQ=255)                                                                    gCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAg                                                                                                                                                                                                                              <  1:73818/300‑1 (MQ=255)                                                                            gTCAATGCGGCGTGCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCTCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCGTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGGCAGACCATAACCGGAAGCGTTAATc                                                                                                                                                                                                                      <  2:135018/300‑1 (MQ=255)                                                                                     gCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATNCGCGTATGGACGCCAAGCGTCAGACCATAAACGGAAGCGTTAATCTGNTCGATc                                                                                                                                                                                                             >  2:651510/1‑300 (MQ=255)                                                                                     gCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATc                                                                                                                                                                                                             >  1:361699/1‑300 (MQ=255)                                                                                     gCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTAAGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGAAGCCAAGCGTCAGAACATAACCGGAAGCGTTAATCTGCTCGATc                                                                                                                                                                                                             >  2:205280/1‑300 (MQ=255)                                                                                                       aCCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTa                                                                                                                                                                                           >  1:209263/1‑300 (MQ=255)                                                                                                                       ccTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGcacca                                                                                                                                                                           >  1:459297/1‑300 (MQ=255)                                                                                                                        cTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGcaccac                                                                                                                                                                          <  1:30357/300‑1 (MQ=255)                                                                                                                          gACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACAt                                                                                                                                                                        >  1:359634/1‑300 (MQ=255)                                                                                                                                                         cgttcGATTTGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCaa                                                                                                                                         <  2:98013/296‑1 (MQ=255)                                                                                                                                                                         agACGGTAGAGATAGAGCGGACTGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTAATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATcc                                                                                                                         >  2:159812/1‑300 (MQ=255)                                                                                                                                                                            cGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGt                                                                                                                      >  1:639226/1‑300 (MQ=255)                                                                                                                                                                             ggTAGAGATAGAGCGTACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGtt                                                                                                                     <  2:464656/300‑1 (MQ=255)                                                                                                                                                                             ggTAGAGATAGAGCGGACCGCCTGCTTTCGTCCCGGTACCGGACAACCCTTCGCCGCCGAATGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGTACCACATGCAGCACCGGATCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGtt                                                                                                                     <  2:228830/300‑1 (MQ=255)                                                                                                                                                                             ggTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGtt                                                                                                                     >  1:18848/1‑300 (MQ=255)                                                                                                                                                                                 gagaTAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGCCCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTATCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGTCAAAGTCATCCAGTTCGAt                                                                                                                 >  2:5585/1‑300 (MQ=255)                                                                                                                                                                                  agaTAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATc                                                                                                                >  1:507405/1‑300 (MQ=255)                                                                                                                                                                                  agaTAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATc                                                                                                                >  2:458856/1‑300 (MQ=255)                                                                                                                                                                                            gACCGCCTGCTTTCTGCCCGGTACCGGACAACCCTTCGCCGCCGGACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAATATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTTgggg                                                                                                      <  2:286209/300‑1 (MQ=255)                                                                                                                                                                                            gACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCgggg                                                                                                      <  1:104557/300‑1 (MQ=255)                                                                                                                                                                                            gACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCgggg                                                                                                      <  2:292865/300‑1 (MQ=255)                                                                                                                                                                                                           gCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGc                                                                                       >  1:6117/1‑300 (MQ=255)                                                                                                                                                                                                                gTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGc                                                                                  >  2:436228/1‑300 (MQ=255)                                                                                                                                                                                                                gTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGc                                                                                  >  1:692497/1‑300 (MQ=255)                                                                                                                                                                                                                gTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACATCTTTTTGCAGTTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCAGCTTTGc                                                                                  >  2:151051/1‑300 (MQ=255)                                                                                                                                                                                                                       aCAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCAc                                                                           <  2:466315/300‑1 (MQ=255)                                                                                                                                                                                                                          aCCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCACTGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCNGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTAACggg                                                                        >  2:186208/1‑300 (MQ=255)                                                                                                                                                                                                                          aCCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACggg                                                                        >  1:614345/1‑300 (MQ=255)                                                                                                                                                                                                                           cccTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGc                                                                       >  1:245308/1‑300 (MQ=255)                                                                                                                                                                                                                                            gCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTccc                                                      >  1:606541/1‑300 (MQ=255)                                                                                                                                                                                                                                            gCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTAGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTccc                                                      >  2:306132/1‑300 (MQ=255)                                                                                                                                                                                                                                                cacaCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCAc                                                  <  1:239151/300‑1 (MQ=255)                                                                                                                                                                                                                                                 acacCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGCTCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCAATTCACGGGCATCTTCGCTGTTTTCCCGCAcc                                                 >  1:209655/1‑300 (MQ=255)                                                                                                                                                                                                                                                   accaaccaCTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGc                                               >  1:257973/1‑300 (MQ=255)                                                                                                                                                                                                                                                          acttcgcCCACCATATTACGTTTAACATACAGATTACCAACATTGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAac                                        <  2:138105/296‑1 (MQ=255)                                                                                                                                                                                                                                                          aCTGCGNCCACCATATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCANGGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCTTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGTTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAac                                        <  2:663639/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aCTGCGCCCACTATATTACGGTTAACATACAGGTTACCAACAGGGGTCGAGCCATTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAACTTCAGACCATAACCGTGAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAac                                        <  2:307680/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ccaTATTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGc                              >  1:646199/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      atatTACGGTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCt                            >  1:345396/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gTTAACATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACg                    >  1:674565/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aCATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGAGAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATg                >  2:485537/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aCATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATg                >  1:410700/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aCATACAGGTTACCAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCAGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATg                >  2:470712/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cAACATGGGTCGAGTCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCANATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCTGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAGAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCACACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGgc   <  2:198414/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               cAACATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGgc   <  1:40373/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               cAACATGGGTCGAGCCAGGGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCTGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGgc   <  2:396255/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aaCATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTCCGCATGGTCTGAATATGgcg  >  1:220245/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                aaCATGGGTCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGgcg  >  1:220328/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    TCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCTCGATCAGCTCTGGTAGCTGGTTACGGTTGTAACGCACCACATGCAGCACCGGACCAAAGACCTCTTTTTGCAATTCGGCAAAGTCATCCAGTTCGATCAGCGTCGGGGCGACAAAGGTGCCGCTTTGCCATTCACGGGCATCTTCGCTGTTTTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCG  >  NC_000913/1075389‑1075975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑