Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,913,979	C→T	D280N (GAC→AAC)	prc ←	tail‑specific protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,913,979	0	C	T	100.0%	205.4 / NA	57	D280N (GAC→AAC)	prc	tail‑specific protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/30);  total (27/30)

CTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGTTTCACGAA  >  NC_000913/1913694‑1914257                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                       cTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGAACATTTTGTTACCAACGCTGATAg                                                                                                                                                                                                                                                                          >  2:483513/1‑300 (MQ=255)   ttGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCt                                                                                                                                                                                                                                                                        <  1:225772/300‑1 (MQ=255)         tCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTActc                                                                                                                                                                                                                                                                  <  1:697604/300‑1 (MQ=255)         gggctcggagatgtgtataagagacagggAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTActc                                                                                                                                                                                                                                                                  <  1:421335/273‑1 (MQ=255)              tGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTANGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCNTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGc                                                                                                                                                                                                                                                             >  2:631346/1‑300 (MQ=255)               gTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCt                                                                                                                                                                                                                                                            >  1:625334/1‑300 (MQ=255)                   aaCCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCg                                                                                                                                                                                                                                                        >  1:170842/1‑300 (MQ=255)                               aGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGAACAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGcca                                                                                                                                                                                                                                            >  1:512645/1‑300 (MQ=255)                                    ggAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTACTTAAGGCAACCCCATCCTCCCGCCGCCCGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGGTAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATc                                                                                                                                                                                                                                       >  1:217016/1‑300 (MQ=255)                                    ggAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTActcctgtctcttatacacatctagcagggg                                                                                                                                                                                                                                       >  2:416072/1‑273 (MQ=255)                                         atCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTNAAATTTCCAGACGAACTTTACTGCCCNTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAAGGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAAtt                                                                                                                                                                                                                                  >  2:531997/1‑300 (MQ=255)                                                       cTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGt                                                                                                                                                                                                                    <  2:408916/300‑1 (MQ=255)                                                         ttCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGtca                                                                                                                                                                                                                  <  1:528662/300‑1 (MQ=255)                                                              ttgaCCGACGGTCTTCACCGACATTGTAACCGCGCGGTCTTTGAGACGAATACGNTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGTTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTNCTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCatcatc                                                                                                                                                                                                             <  2:738156/297‑1 (MQ=255)                                                                        gTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGTTCAACGTTACAGTACGGGTCTTGTTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGTCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTgcagc                                                                                                                                                                                                   <  2:20199/300‑1 (MQ=255)                                                                          cTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCAc                                                                                                                                                                                                 <  1:794016/300‑1 (MQ=255)                                                                          cTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACAAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGTTGCCGGACCACCTGCCACCATGGAATGGGTAACGGTGTAGtgggtgtcggtttgtagtgg                                                                                                                                                                                                 <  2:121523/300‑20 (MQ=255)                                                                                     ttttAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATAc                                                                                                                                                                                      <  1:784638/300‑1 (MQ=255)                                                                                                  gTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACaa                                                                                                                                                                         >  1:722326/1‑300 (MQ=255)                                                                                                                tACGTTCACGGGTCAACGTTACAGTACTGGTCTTGGTCCCCTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAgtgt                                                                                                                                                           <  2:685589/300‑1 (MQ=255)                                                                                                                  cGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTAGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTg                                                                                                                                                         >  2:519260/1‑300 (MQ=255)                                                                                                                     tCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAAc                                                                                                                                                      <  2:273477/300‑1 (MQ=255)                                                                                                                         gggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAGTTGATAACGGTGTAGTCATCATCCATTNGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGtt                                                                                                                                                  >  2:588530/1‑300 (MQ=255)                                                                                                                          ggTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTAAGTGTTGAACTGTTc                                                                                                                                                 >  2:243920/1‑300 (MQ=255)                                                                                                                               aCGTTANAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAANTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTAt                                                                                                                                            <  2:659616/300‑1 (MQ=255)                                                                                                                               aCGTTACAGTGCGGGTCTTGGTCCCTTTACCAGCAGTTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACTTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTAt                                                                                                                                            <  2:141480/300‑1 (MQ=255)                                                                                                                               aCGTTACAGTACGGTTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTAt                                                                                                                                            <  2:366140/300‑1 (MQ=255)                                                                                                                               aCGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCGTCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCCTTTCAGTGTTGAACTGTTCGGTAt                                                                                                                                            >  1:240381/1‑300 (MQ=255)                                                                                                                               aCGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTAt                                                                                                                                            <  1:295566/300‑1 (MQ=255)                                                                                                                                  ttACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAc                                                                                                                                         >  1:411952/1‑300 (MQ=255)                                                                                                                                    aCAGTACGGTTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAcgc                                                                                                                                       <  2:434666/300‑1 (MQ=255)                                                                                                                                    aCAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAcgc                                                                                                                                       <  1:258295/300‑1 (MQ=255)                                                                                                                                    aCAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAcgc                                                                                                                                       <  2:455059/300‑1 (MQ=255)                                                                                                                                       gTACGGGTCTTGGTCCCTTTACCAGCAGGTANAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCNTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTACAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCggg                                                                                                                                    >  2:565652/1‑300 (MQ=255)                                                                                                                                       gTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCGTCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTAATCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCATTGTGCCAATACCTTACAGCGACAAATTCATTTCGGTGTTGAATTGTTCGGCATTACGCggg                                                                                                                                    >  2:119312/1‑300 (MQ=255)                                                                                                                                              tCTTGGTCCCTTTACCAGCAGTTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAATGCAACCACATCATCAAGACGCCAGCCAATCACTTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGAt                                                                                                                             <  1:164936/300‑1 (MQ=255)                                                                                                                                              tCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGAt                                                                                                                             <  1:245591/300‑1 (MQ=255)                                                                                                                                                tgggTCCCTTGACTAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAg                                                                                                                           <  2:119036/298‑1 (MQ=255)                                                                                                                                                   gTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAATGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTg                                                                                                                        <  2:411872/300‑1 (MQ=255)                                                                                                                                                       cTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCNGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTAt                                                                                                                    >  2:188260/1‑300 (MQ=255)                                                                                                                                                       cTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTAt                                                                                                                    >  1:298417/1‑300 (MQ=255)                                                                                                                                                        tttACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATg                                                                                                                   <  1:350239/300‑1 (MQ=255)                                                                                                                                                                                aCGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCANCTGCCACCATCGAATTGATAACGGTGNAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGc                                                                                           <  2:544256/300‑1 (MQ=255)                                                                                                                                                                                    aCTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTc                                                                                       >  1:287380/1‑300 (MQ=255)                                                                                                                                                                                     cTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAAGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCa                                                                                      >  2:338348/1‑300 (MQ=255)                                                                                                                                                                                          cTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGcc                                                                                 <  2:448138/300‑1 (MQ=255)                                                                                                                                                                                                           aattaaGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTc                                                                >  2:320013/1‑300 (MQ=255)                                                                                                                                                                                                           aattaaGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAAGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTc                                                                >  2:372043/1‑300 (MQ=255)                                                                                                                                                                                                                     aCCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTTTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGTACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCATCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGtt                                                      <  2:301026/300‑1 (MQ=255)                                                                                                                                                                                                                     aCCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCATCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGtt                                                      <  2:338783/300‑1 (MQ=255)                                                                                                                                                                                                                                                      aCCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAgcgg                     >  2:725949/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gCCTGTTTGACCAGCACCGACAATCGTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGt          <  2:169899/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 gCCTGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAACACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGt          <  1:727335/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    tGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGTTTc       >  1:88101/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    tGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGTTTc       >  2:723883/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    tGTTTGACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATNACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTAGTTGCAAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGTTTc       >  2:191142/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gACCAACACCGACAATTTTGTTACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGTTTCACGaa  >  1:76929/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                       CTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCGAGTCAGGGTTTCACGAA  >  NC_000913/1913694‑1914257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑