Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,864,475	G→A	R209H (CGC→CAC)	yeaD →	putative aldose 1‑epimerase YeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,475	0	G	A	100.0%	255.6 / NA	73	R209H (CGC→CAC)	yeaD	putative aldose 1‑epimerase YeaD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/33);  total (40/33)

GATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGC  >  NC_000913/1864180‑1864766                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    gATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACgtg                                                                                                                                                                                                                                                                                                 <  2:80758/300‑1 (MQ=255)     cTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTNAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATc                                                                                                                                                                                                                                                                                             >  1:570262/1‑300 (MQ=255)      tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAATAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCt                                                                                                                                                                                                                                                                                            <  2:301016/300‑1 (MQ=255)      tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCt                                                                                                                                                                                                                                                                                            <  2:760568/300‑1 (MQ=255)       gATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTg                                                                                                                                                                                                                                                                                           >  1:779052/1‑300 (MQ=255)           gCGTCGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCATGACCGTACCGATCACGTGTATCTGAATc                                                                                                                                                                                                                                                                                       >  1:98713/1‑300 (MQ=255)           gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATc                                                                                                                                                                                                                                                                                       >  1:623065/1‑300 (MQ=255)           gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTAATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCATGACCGTACCGATCACGTGTATCTGAATc                                                                                                                                                                                                                                                                                       >  2:298785/1‑300 (MQ=255)            cGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATAGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATcc                                                                                                                                                                                                                                                                                      <  2:684905/300‑1 (MQ=255)                       cTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGACACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGACAGAAAATGTACT‑ACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAg                                                                                                                                                                                                                                                                          >  1:736576/1‑300 (MQ=255)                       cTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCa                                                                                                                                                                                                                                                                           >  2:765288/1‑300 (MQ=255)                       cTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCa                                                                                                                                                                                                                                                                           >  1:503418/1‑300 (MQ=255)                                       aaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTACCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAAtgatga                                                                                                                                                                                                                                                           >  1:135149/1‑300 (MQ=255)                                                 aCGAAANAATTCTGGCCGCACGACTGTACGCTGTTAGCGCATTTCCGCTTGGGTNAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAAt                                                                                                                                                                                                                                                 <  2:661341/300‑1 (MQ=255)                                                 aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAAt                                                                                                                                                                                                                                                 <  1:37895/300‑1 (MQ=255)                                                 aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCGGAAt                                                                                                                                                                                                                                                 <  1:466929/300‑1 (MQ=255)                                                        aaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTTTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGtatta                                                                                                                                                                                                                                          <  1:272623/300‑1 (MQ=255)                                                          ttCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATc                                                                                                                                                                                                                                        <  1:620056/300‑1 (MQ=255)                                                                    aCGACTCTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGNCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACATGTATCGGAATCCACAAGATTGCAGCGTGATTAATGAGGAAGCGCTGAATCGTATTATCGCCGTAGGcc                                                                                                                                                                                                                              <  2:186980/300‑1 (MQ=255)                                                                       aCTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGccacc                                                                                                                                                                                                                           >  1:214921/1‑300 (MQ=255)                                                                                tGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCatcatc                                                                                                                                                                                                                  <  1:150956/300‑1 (MQ=255)                                                                                     gcgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAAc                                                                                                                                                                                                             <  2:132519/300‑1 (MQ=255)                                                                                     gcgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAAc                                                                                                                                                                                                             <  1:338002/300‑1 (MQ=255)                                                                                     gcgcATTTCCGCACGTGTAAAACCCGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAAc                                                                                                                                                                                                             <  1:723909/300‑1 (MQ=255)                                                                                      cgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACg                                                                                                                                                                                                            <  1:423363/300‑1 (MQ=255)                                                                                          tttCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTAATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGt                                                                                                                                                                                                        >  2:510432/1‑300 (MQ=255)                                                                                                   gggTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGAAGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGaa                                                                                                                                                                                               >  2:313454/1‑300 (MQ=255)                                                                                                     gTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAAcc                                                                                                                                                                                             <  1:675863/300‑1 (MQ=255)                                                                                                     gTAAAACTTGTGAAATCGACCTTGAATCACATGGCGAATTTGAAACTACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAAcc                                                                                                                                                                                             <  2:42328/300‑1 (MQ=255)                                                                                                            ttGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGAcc                                                                                                                                                                                      <  1:740217/300‑1 (MQ=255)                                                                                                                           tGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTCAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGANTGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACNTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAg                                                                                                                                                                       <  2:645954/300‑1 (MQ=255)                                                                                                                                           tttGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCg                                                                                                                                                       <  1:43526/300‑1 (MQ=255)                                                                                                                                              gAAACCACCTCTTCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGNTTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGNGCTTTCAATTAGCATGGGCGATATGCCGgat                                                                                                                                                    <  2:562160/300‑1 (MQ=255)                                                                                                                                              gAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGgat                                                                                                                                                    <  1:691826/300‑1 (MQ=255)                                                                                                                                                aaCCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGgatga                                                                                                                                                  >  2:724855/1‑300 (MQ=255)                                                                                                                                                   caccTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATgg                                                                                                                                               <  1:465261/300‑1 (MQ=255)                                                                                                                                                     ccTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCt                                                                                                                                             >  1:436108/1‑300 (MQ=255)                                                                                                                                                     ccTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCATGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCt                                                                                                                                             >  2:520763/1‑300 (MQ=255)                                                                                                                                                          gCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTNCCATGACCGTACCGATCACGTGTATCNGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGCCCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACaaa                                                                                                                                        >  2:541459/1‑300 (MQ=255)                                                                                                                                                          gCCCTGCATACCTACTTTAACGTGGGTGATANCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTNCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACaaa                                                                                                                                        >  2:560566/1‑300 (MQ=255)                                                                                                                                                                       aCTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTtgtg                                                                                                                           >  2:389541/1‑300 (MQ=255)                                                                                                                                                                       aCTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGAACGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGCTtgtg                                                                                                                           >  2:213376/1‑300 (MQ=255)                                                                                                                                                                       aCTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCAACGGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTCtgtg                                                                                                                           >  2:151777/1‑300 (MQ=255)                                                                                                                                                                               gTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCAGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACg                                                                                                                   >  1:199217/1‑300 (MQ=255)                                                                                                                                                                                 gggTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGNATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTNGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGc                                                                                                                 >  2:629993/1‑300 (MQ=255)                                                                                                                                                                                      atatCGATAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACg                                                                                                            >  2:111293/1‑300 (MQ=255)                                                                                                                                                                                         tagCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAAAATTTGTTTGTGTAGAAACGGCTTACGCtt                                                                                                         >  2:49715/3‑300 (MQ=255)                                                                                                                                                                                         tCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAANCCACAAGATTGCAGCGTGATTAATGANGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGATTACGCtt                                                                                                         >  2:543245/1‑300 (MQ=255)                                                                                                                                                                                               aGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGaaa                                                                                                   <  1:687691/300‑1 (MQ=255)                                                                                                                                                                                                      cGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCAGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCaaaa                                                                                            >  2:403021/1‑300 (MQ=255)                                                                                                                                                                                                        tCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAANCGTATTATCGCCGTAGGCCACCAGCANCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAg                                                                                          <  2:539359/300‑1 (MQ=255)                                                                                                                                                                                                             gggctgggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACNGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTANAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGAcc                                                                                     >  2:731227/1‑300 (MQ=255)                                                                                                                                                                                                                   ggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCAGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAagag                                                                               >  1:19046/1‑300 (MQ=255)                                                                                                                                                                                                                          gCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTg                                                                        >  1:687872/1‑300 (MQ=255)                                                                                                                                                                                                                             tCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGcac                                                                     <  1:261069/300‑1 (MQ=255)                                                                                                                                                                                                                                ttGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATc                                                                  >  1:264214/1‑300 (MQ=255)                                                                                                                                                                                                                                          gAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGAACAAAGAGAAACCTGCAAATCTGGCGCAATc                                                        >  2:113141/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCg                                           >  1:233161/1‑300 (MQ=255)                                                                                                                                                                                                                                                        aaaaTGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATCGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACACCTGGCGCAATCCCTTCGCGTTgaga                                          >  2:69281/1‑297 (MQ=255)                                                                                                                                                                                                                                                                       aTGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGt                           >  2:469900/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       aTGGTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACANTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGt                           >  2:690508/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTaa                        >  1:221821/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTATTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCAGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTaa                        >  1:164067/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            aTTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAAtg                      <  1:778707/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            aTTCAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTAAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGCTAACTTACGTTAAtg                      >  2:385186/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               cAGACCTTCCCTGGCCGTACCGATCACGTGTATCTGAATCCACAAGATTGCATCTTGATTAATGGTGAAGCGCTGAAGCGTATGATCGAAGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAAtgttg                   <  2:207807/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               cAGACCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAAtgttg                   <  2:493793/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gACCTTCCCTGACCGTACCGATCACGTGTAGCTGAATCCACAAGATTGCATCGTAATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCTGGACCGGCGCTTTCAATTGGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTtgtg                 <  2:98101/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aCCTTCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTtgtgt                <  1:651184/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aCCTTCCCTGACCGGACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTtgtgt                <  1:651193/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCg            >  1:638890/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      tCCCTGACCGTACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCg            >  2:474040/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                tACCGATCACGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGc  <  1:301136/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    GATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGC  >  NC_000913/1864180‑1864766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑