Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,475	G→A	L496L (CTG→TTG)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,475	0	G	A	100.0%	162.1 / NA	45	L496L (CTG→TTG)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

CGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTT  >  NC_000913/1845179‑1845769                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       cGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGTTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAatgt                                                                                                                                                                                                                                                                                                     <  2:376149/300‑1 (MQ=255)      gCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCAt                                                                                                                                                                                                                                                                                                <  1:664499/300‑1 (MQ=255)            aGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGNGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTaa                                                                                                                                                                                                                                                                                          >  1:588471/1‑300 (MQ=255)                              gCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTggg                                                                                                                                                                                                                                                                        <  2:680716/300‑1 (MQ=255)                                ttttCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTACGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGtt                                                                                                                                                                                                                                                                      <  1:168974/300‑1 (MQ=255)                                           ttGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTAc                                                                                                                                                                                                                                                           <  1:339639/300‑1 (MQ=255)                                              cGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCAc                                                                                                                                                                                                                                                        >  1:484442/1‑300 (MQ=255)                                              cGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCAc                                                                                                                                                                                                                                                        >  2:17659/1‑300 (MQ=255)                                                     aCCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGTGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAACATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCt                                                                                                                                                                                                                                                 >  2:405959/1‑300 (MQ=255)                                                              cAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTcacac                                                                                                                                                                                                                                        <  1:32181/300‑1 (MQ=255)                                                                gTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAg                                                                                                                                                                                                                                      >  1:745925/1‑300 (MQ=255)                                                                       gTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTNCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCa                                                                                                                                                                                                                               >  1:584064/1‑300 (MQ=255)                                                                       gTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCa                                                                                                                                                                                                                               >  1:414833/1‑300 (MQ=255)                                                                       gTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCa                                                                                                                                                                                                                               >  2:754832/1‑300 (MQ=255)                                                                                   cgcgtcgcCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGTAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGcc                                                                                                                                                                                                                   <  2:23958/300‑1 (MQ=255)                                                                                   cgcgtcgcCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCATCTGTCCACAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGACTTTCGcc                                                                                                                                                                                                                   >  1:271122/1‑300 (MQ=255)                                                                                        cgcCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCcaccac                                                                                                                                                                                                              <  2:611533/300‑1 (MQ=255)                                                                                            aTCTCCTGCAGCGAATGGAATAGCGCTTTTCCGGCGGCGTCGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGTAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGc                                                                                                                                                                                                          <  2:684548/300‑1 (MQ=255)                                                                                                                                      gagtggaGATAGCTCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCTGCACGCGTTGCCTCTGTCCCCAGACCATCGTTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCt                                                                                                                                                                <  2:389224/300‑1 (MQ=255)                                                                                                                                       agtggaTATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTa                                                                                                                                                               >  2:756500/1‑300 (MQ=255)                                                                                                                                         tggaTATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAAc                                                                                                                                                             <  1:526652/300‑1 (MQ=255)                                                                                                                                                       gctagatgtggataagagacagttGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTGTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGGTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTGTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTCGCTGCCTAACAGCGTGCGCCAGcc                                                                                                                                               <  2:329468/278‑1 (MQ=255)                                                                                                                                                       gcgagatgtgtataagagacagttGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGcc                                                                                                                                               <  2:330509/278‑1 (MQ=255)                                                                                                                                                             ggTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                                         <  2:616904/300‑1 (MQ=255)                                                                                                                                                                          cGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgc                                                                                                                            >  2:514631/1‑300 (MQ=255)                                                                                                                                                                            cTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAACAAGAAAACgcgc                                                                                                                          >  1:414773/1‑300 (MQ=255)                                                                                                                                                                             ttGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGccctgtctcttatacacatctccg                                                                                                                         >  1:333404/1‑278 (MQ=255)                                                                                                                                                                             ttGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCAACTTTTTCACACAGCAACTCATCGCCTTTCGCCAACACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCCACCTAACAGCGTGCGCCAGccctgtctatcatacacatctccg                                                                                                                         >  1:332345/1‑278 (MQ=255)                                                                                                                                                                                                                 tctatCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATTGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACTACTTCCCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAACCGCGCTTTAGCAACAAATTTGCCTTTGGCAATGTCCAGTTCg                                                                                     >  2:421862/5‑300 (MQ=255)                                                                                                                                                                                                                    gTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGTAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCAATTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCTACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCCTCGCGCTCTCTGCTGCCTACCAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGCCCAGTCCGATa                                                                                  >  2:51917/1‑300 (MQ=255)                                                                                                                                                                                                                    gTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATa                                                                                  >  2:246169/1‑300 (MQ=255)                                                                                                                                                                                                                    gTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATa                                                                                  >  1:152325/1‑300 (MQ=255)                                                                                                                                                                                                                     tCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAANGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCCCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATaa                                                                                 <  2:585591/300‑1 (MQ=255)                                                                                                                                                                                                                             ccATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTAAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTg                                                                         >  2:772154/1‑300 (MQ=255)                                                                                                                                                                                                                                            aggaTCCTTTTCAGATCTTTATCCTGCACAAAGCGCACGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGATTTGCCGCTCTACCACTTCACCTTTTTCGCACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCATACGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCGTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATcc                                                          <  2:381823/300‑1 (MQ=255)                                                                                                                                                                                                                                                tCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                                                      <  1:455605/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tttttCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAg                                                    <  2:306627/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tttttCAGATCTTTATCCTACACAAAGCGCTCGATCCCGGTCACCACCGAAAGCAATGTTGAATCGGTAAAATGGCGCGGCGCTTGGGTTGGCAATTTGACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAg                                                    <  2:686052/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCa                                             >  1:115717/1‑300 (MQ=255)                                                                                                                                                                                                                                                           tCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATc                                           >  1:102217/1‑300 (MQ=255)                                                                                                                                                                                                                                                           tCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACAACTTCACCTTTTTCACACAGCAACTCATCGACTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCCTCTAGGCAGAATTGCATc                                           >  1:687816/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         aaaGCGNGCGATCCCGGTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGNGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTGCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGc                             <  2:666615/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ggTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAc              <  2:47693/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ggTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAc              <  1:738590/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gTCATCGCCGAAAGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGTCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACATCGCATCCGGGCAGAATCGCATCAGATACTGACGGGCAATCAGGTTATAGAcc             >  1:166425/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    aaGCAATGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGtt  >  1:253293/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       CGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTT  >  NC_000913/1845179‑1845769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑