Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,887	Δ1 bp	coding (678/1221 nt)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,887	0	C	.	100.0%	216.9 / NA	42	coding (678/1221 nt)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/12);  total (30/12)

CACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGATCGATATGGG  >  NC_000913/1667611‑1668176                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                  cACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTNTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAacc                                                                                                                                                                                                                                                                           >  1:567006/1‑300 (MQ=255)      cGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCAAGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTg                                                                                                                                                                                                                                                                      >  2:509/1‑300 (MQ=255)              atgTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACAt                                                                                                                                                                                                                                                              <  1:694122/300‑1 (MQ=255)                                 cgccgcGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGc                                                                                                                                                                                                                                           <  1:521705/300‑1 (MQ=255)                                     gcgcAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAAACACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCa                                                                                                                                                                                                                                       >  2:476023/1‑300 (MQ=255)                                               gccTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCct                                                                                                                                                                                                                             >  1:489759/1‑300 (MQ=255)                                                  tGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTg                                                                                                                                                                                                                          >  1:461963/1‑300 (MQ=255)                                                   gACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGc                                                                                                                                                                                                                         >  2:342435/1‑300 (MQ=255)                                                                         cGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATCTACACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGGGGCACCACACAAGGCCTCTGCCATCGTCCATGCGCTGATATCa                                                                                                                                                                                                   >  2:286625/1‑300 (MQ=255)                                                                                  aTGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAata                                                                                                                                                                                          >  1:453133/1‑300 (MQ=255)                                                                                  aTGTAACATCGAGCCCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGAACATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGATGAata                                                                                                                                                                                          >  1:453217/1‑300 (MQ=255)                                                                                                       ttgattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgc                                                                                                                                                                     <  1:522756/300‑1 (MQ=255)                                                                                                         gattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctg                                                                                                                                                                   >  1:198280/1‑300 (MQ=255)                                                                                                              aGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCa                                                                                                                                                              >  1:418705/1‑300 (MQ=255)                                                                                                               gACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAg                                                                                                                                                             <  1:656776/300‑1 (MQ=255)                                                                                                                     gCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCANGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAAAAAGGCCTCTGCCATCGTCCATGCGCTGATATCACGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCta                                                                                                                                                       >  2:180363/1‑299 (MQ=255)                                                                                                                                             tcacgctttcGATGGTTGCTAGGCAGCCGTGATTCCCGCAATAACATCTTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACTAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGGAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATc                                                                                                                               <  2:149928/291‑1 (MQ=255)                                                                                                                                                cGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTc                                                                                                                            >  1:111171/1‑300 (MQ=255)                                                                                                                                                       gATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAAc                                                                                                                     <  2:784039/300‑1 (MQ=255)                                                                                                                                                                      gCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTAGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACa                                                                                                      >  2:510558/1‑300 (MQ=255)                                                                                                                                                                          tGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATAc                                                                                                  <  1:610268/300‑1 (MQ=255)                                                                                                                                                                                                          gTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGtt                                                                  >  1:86418/1‑300 (MQ=255)                                                                                                                                                                                                              aacTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGACCGGCATGCGATGTACACTACCATCTTCCGTATCAATAATTCCCGGCAAGGTTATgg                                                              >  2:133789/3‑300 (MQ=255)                                                                                                                                                                                                               ccTGTGTGTTGCCTATTTCCACGAGACGACTACTGCCTGCGTGTAACAGGTGACCGTCGGTAATGACGCC‑GCCCCCACGTTGGGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCTGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGc                                                             <  2:223453/300‑1 (MQ=255)                                                                                                                                                                                                               ccTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGc                                                             <  1:337926/300‑1 (MQ=255)                                                                                                                                                                                                               ccTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATCCCCGGCAAGGTTATGGc                                                             >  2:7363/1‑300 (MQ=255)                                                                                                                                                                                                               ccTGTGNGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACNATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGc                                                             <  2:655214/300‑1 (MQ=255)                                                                                                                                                                                                                 ctgcgagatgtgtataagagacagctactGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGTGGCATCTCTTTTACATCCTCATAGAACGGCATGCGATGTACAATAACATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCaa                                                           >  1:346343/25‑300 (MQ=255)                                                                                                                                                                                                                 ctgcgagatgtgtataagagacagctactGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGTGATGTACAATACCCTTTTCCGTCTCAATAATTCCCGGCAAGGTTATGGCaa                                                           >  1:346186/25‑300 (MQ=255)                                                                                                                                                                                                                  gtgtgtgGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAAt                                                          >  2:459035/1‑300 (MQ=255)                                                                                                                                                                                                                  gtgtgtgGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAAt                                                          >  1:693603/1‑300 (MQ=255)                                                                                                                                                                                                                                      agaCTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTAGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTGGAAGCTCCAGtt                                      >  2:70485/1‑300 (MQ=255)                                                                                                                                                                                                                                           actGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTc                                 >  1:341891/1‑300 (MQ=255)                                                                                                                                                                                                                                                 gcctgcGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCACGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCtggtgg                           >  2:345192/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gcGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCggc                       >  2:386926/1‑299 (MQ=255)                                                                                                                                                                                                                                                          tAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATaaaa                  >  2:392266/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCNCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATaaaaaa                >  1:539831/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGAt           <  1:348061/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aCCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGATCg         >  1:611793/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    ccATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGATCGa        <  1:316155/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGATCGATATgg   >  1:157342/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGATCGATATggg  >  1:235157/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                  CACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGGTTATGGCAATCGAAGTTAGACGCTCAAGTTTTTTCTGGTGGCGGATAAAAAACTGATCGATATGGG  >  NC_000913/1667611‑1668176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑