Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	734,412	C→T	intergenic (+310/‑1238)	ybfB → / → ybfC	uncharacterized protein YbfB/uncharacterized protein YbfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	734,412	0	C	T	100.0%	174.2 / NA	64	intergenic (+310/‑1238)	ybfB/ybfC	uncharacterized protein YbfB/uncharacterized protein YbfC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/33);  total (31/33)

TATTATATGTAACCTGGGCATTGATATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCC  >  NC_000913/734123‑734696                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                             tattatATGTAACCTGGGCATTGATATCCCGTATGCCACAGACCCGGCAGGTAANCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGTAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGNCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAg                                                                                                                                                                                                                                                                                    <  2:756633/300‑1 (MQ=255)                 ggCATTGATATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCNCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGNAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGAc                                                                                                                                                                                                                                                                    <  2:659964/300‑1 (MQ=255)                 ggCATTGATATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGAc                                                                                                                                                                                                                                                                    <  1:148774/300‑1 (MQ=39)                                    ccACAGACCCGGCAGGTAACCGCCTGCCCGAACCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTAACGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTAAACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTgg                                                                                                                                                                                                                                                 >  2:442307/1‑300 (MQ=19)                                       caGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTTGCCGGATAACCGTATCGCCCTTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGTTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCaa                                                                                                                                                                                                                                              <  2:693328/300‑1 (MQ=19)                                          aCCCGGCAGGTAACCTCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCAATATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCACCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCACTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAAc                                                                                                                                                                                                                                           <  2:153592/300‑1 (MQ=255)                                                  ggTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCACGGAAGGGGTTATCCGCACGGATGATGANCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGAACCGCTGGGCAGCAGGGTGGCAAAACGGGTGTgg                                                                                                                                                                                                                                   >  2:194624/1‑300 (MQ=255)                                                  ggTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTAACGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCAGGAAGGGGTTATCCGCACGGATGATGANCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCAGCTATCTTTACGACCCGCTGGGCAGCAGGGTGGCAAAACGGGTGTgg                                                                                                                                                                                                                                   >  2:194376/1‑300 (MQ=255)                                                                            gCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCNTCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTg                                                                                                                                                                                                         <  1:597551/300‑1 (MQ=37)                                                                             cACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCAGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGTACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTCCGAAACGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTgg                                                                                                                                                                                                        >  2:284402/1‑300 (MQ=255)                                                                             cACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCAAGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTANCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACNACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACAGGCTgg                                                                                                                                                                                                        >  2:648405/1‑300 (MQ=255)                                                                               cccGGACAGCACCCGCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGTGCTGGAt                                                                                                                                                                                                      <  2:310103/300‑1 (MQ=255)                                                                                                gCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAAc                                                                                                                                                                                     >  1:701943/1‑300 (MQ=25)                                                                                                               aCCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGACAGCCGCNATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGt                                                                                                                                                                      >  2:730536/1‑300 (MQ=25)                                                                                                                ccGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTa                                                                                                                                                                     <  1:245150/300‑1 (MQ=25)                                                                                                                   tATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACTGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACgg                                                                                                                                                                  <  2:52839/300‑1 (MQ=25)                                                                                                                         ccGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGa                                                                                                                                                            <  1:504700/300‑1 (MQ=25)                                                                                                                                  gcACTATCTTTACCGGTCTGACCGTCACGGCNGGCTGACAGAGAAAACCGACCTCATCCAGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGNTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGCAAGCCGCTATCTTTACGACCCGCTGGGCCGCCGGGTGGCACACCGGGTGTGGCGACGTGAACGGGACCTGACGGGCCGGATGTCGCTGTCACGGAAACCGCAAGTAACCTAGTACGGTTGGGACGGCGACCg                                                                                                                                                   >  2:590497/1‑300 (MQ=11)                                                                                                                                  gcACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCAGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCg                                                                                                                                                   >  1:51611/1‑300 (MQ=25)                                                                                                                                       aTCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGNCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGa                                                                                                                                              >  1:576120/1‑300 (MQ=25)                                                                                                                                       aTCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGa                                                                                                                                              >  2:379060/1‑300 (MQ=25)                                                                                                                                       aTCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGa                                                                                                                                              >  1:826551/1‑300 (MQ=25)                                                                                                                                       aTCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTATCACGGAAACCGCAAGTGACCTGGTAAGGTTGGGACGGCGACCGCCTGa                                                                                                                                              >  2:423044/1‑300 (MQ=255)                                                                                                                                       aTCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCAGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGa                                                                                                                                              >  1:143383/1‑300 (MQ=25)                                                                                                                                         cTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGAcc                                                                                                                                            >  1:382868/1‑300 (MQ=25)                                                                                                                                          tttACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCa                                                                                                                                           <  1:372376/300‑1 (MQ=25)                                                                                                                                           ttACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCAc                                                                                                                                          <  2:371611/300‑1 (MQ=25)                                                                                                                                                  tATGACCGTTACTGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGTGGTTATCCGCACGGATGATGAGCACACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACTACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACTGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGTCGACCGCCTGACCACGATACAg                                                                                                                                   <  2:159066/300‑1 (MQ=11)                                                                                                                                                     gACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAAc                                                                                                                                <  1:16439/300‑1 (MQ=25)                                                                                                                                                        cGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGAc                                                                                                                             >  2:738139/1‑300 (MQ=25)                                                                                                                                                          tCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTAAACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCAAGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAg                                                                                                                           >  2:699685/1‑300 (MQ=255)                                                                                                                                                               gCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAAccc                                                                                                                      <  1:510216/300‑1 (MQ=25)                                                                                                                                                                                          tCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACNGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCNTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGa                                                                                           >  2:756139/1‑300 (MQ=21)                                                                                                                                                                                               gAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTc                                                                                      <  2:361127/300‑1 (MQ=21)                                                                                                                                                                                                   gggTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACNGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATNCAGACGATTTATCAGCCGGGGAGCTTCACGc                                                                                  <  2:590033/300‑1 (MQ=21)                                                                                                                                                                                                        aTCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGGGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACtc                                                                             <  1:22072/300‑1 (MQ=21)                                                                                                                                                                                                        aTCCGCACGGAGGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCACGGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAGGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACtc                                                                             <  1:22231/300‑1 (MQ=255)                                                                                                                                                                                                                ggATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGt                                                                     <  1:655167/300‑1 (MQ=21)                                                                                                                                                                                                                      tgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAAc                                                               <  2:214818/300‑1 (MQ=21)                                                                                                                                                                                                                      tgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAAc                                                               <  1:47034/300‑1 (MQ=21)                                                                                                                                                                                                                                caccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGt                                                     >  1:712024/1‑300 (MQ=21)                                                                                                                                                                                                                                caccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTAAACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGAAGTGAACGGGACCTGACGGGCTGGATGTCGATGTCACGGAAACCGCAAGTGACTTGGTACGGTTGGGACGGCGAACGCCTGACCACGATAAAGAACGACAGAACCCGCATACAGACGATTTATCAGCCGGGGAGCTTAACGACAATAATCAGGGTTGaaatcgaaacaggt                                                     >  2:94041/1‑289 (MQ=11)                                                                                                                                                                                                                                  ccGGTACCGTTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCCTTACGACCCGCTGGGCCGCAAGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTAACGTGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTCCGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGCAAAGTTATCAGCAGGGAAGCTTCAAGCCAATCATAAGGATGGAAATCGCCCACGGTGa                                                   >  2:530081/1‑300 (MQ=11)                                                                                                                                                                                                                                    ggTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGc                                                 >  1:132486/1‑300 (MQ=21)                                                                                                                                                                                                                                     gTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGTTGGCAAAACNGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGNCCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCt                                                <  2:660083/300‑1 (MQ=21)                                                                                                                                                                                                                                     gTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCt                                                <  2:286664/300‑1 (MQ=21)                                                                                                                                                                                                                                           ttACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCACCTGACCACGATACAGAACGACCGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGaa                                          >  2:317887/1‑300 (MQ=19)                                                                                                                                                                                                                                                  aGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAg                                   >  1:770808/1‑300 (MQ=21)                                                                                                                                                                                                                                                  aGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGAACGCCTGACCACGATACAGAACGACAGCACCCGCATCCAGACGATTTATCAGCAGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAg                                   >  1:329791/1‑300 (MQ=255)                                                                                                                                                                                                                                                  aGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGACGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAg                                   >  1:806077/1‑300 (MQ=21)                                                                                                                                                                                                                                                     cAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAgcgc                                <  1:114650/300‑1 (MQ=21)                                                                                                                                                                                                                                                          ccGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAg                           <  1:658306/300‑1 (MQ=21)                                                                                                                                                                                                                                                            ggCTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGcc                         >  2:810483/1‑300 (MQ=21)                                                                                                                                                                                                                                                              cTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCAGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTg                       >  1:276119/1‑300 (MQ=21)                                                                                                                                                                                                                                                              cTGGTGCACTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCAAGGAAACCGCAAGTGAACTGGTACGGTTGGGACGGCGAACGCCTGCCCCCGATACAGAACGAAAGAAACCGTATCCAGAGGGTTTATCAGACGGGGAGCTTAAAGACAATCATCAAGGTTGAAATCGACACCGGTGAGCTGGCGAACGCGCAGTGCCGCAGCCTg                       >  2:220062/1‑300 (MQ=11)                                                                                                                                                                                                                                                                      cTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACTGTACCTGACGGGCTGGATTTCGCTGTCACGGAAACCGCAATTGACCTGGTACGGTTTGGACGTCTACCGCCTGGCCACGATACAGAACGACAGAACCCGCATCCAGACGATTTCTCAGCCTGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATAc               <  2:64466/300‑1 (MQ=11)                                                                                                                                                                                                                                                                      cTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAATGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATAc               <  1:809062/300‑1 (MQ=21)                                                                                                                                                                                                                                                                      cTACACGCGGACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATAc               <  1:692229/300‑1 (MQ=21)                                                                                                                                                                                                                                                                              gtacacAATATGCAGAGCCGTTGTTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGTTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTTTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTcagc       <  2:127865/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              ggACACAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTcagc       <  1:9003/300‑1 (MQ=21)                                                                                                                                                                                                                                                                                acacAATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTcagcag     <  2:510074/300‑1 (MQ=21)                                                                                                                                                                                                                                                                                acacAACATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGTGCCGCAGGGTGGCAAAACGGGTTTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTTTCACGGAAACCGCAAGTGACCTGGTACGGTTTGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCCGGCGGATACCCTTcagcag     <  2:239982/300‑1 (MQ=11)                                                                                                                                                                                                                                                                                   caATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGTTGGGACGGCGACCGCCTGACCCCGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGACGACGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGAGGATACCCTTAAGCAGtac  >  2:312517/1‑298 (MQ=11)                                                                                                                                                                                                                                                                                   caATATGCAGAGCCGTTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGAAAAACGGGTGTGGCGAAGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGATACGGTTGGGACGGCGACTGCCTGACCACGATACAAGACGAAAGAACCCGCATCCAGACGATTTATCAGCCGAGGAGCGTCACGCCACTAATCAGGGTTGAAACCGACAACGGTGAGCTGGCAAAAACGCAACGCCGCAGACTGGCGGATCCCCTTCagtagtac  >  2:268934/1‑298 (MQ=11)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                             TATTATATGTAACCTGGGCATTGATATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCC  >  NC_000913/734123‑734696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑