Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,094,504	G→A	intergenic (‑232/+219)	rhaD ← / ← rhaA	rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,094,504	0	G	A	100.0%	154.6 / NA	49	intergenic (‑232/+219)	rhaD/rhaA	rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/20);  total (29/20)

CAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATC  >  NC_000913/4094211‑4094797                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      cAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGAt                                                                                                                                                                                                                                                                                                 <  2:218791/300‑1 (MQ=255)      gTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGc                                                                                                                                                                                                                                                                                            <  1:504390/300‑1 (MQ=255)        cGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCAc                                                                                                                                                                                                                                                                                          <  1:538090/300‑1 (MQ=255)            ggtggCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGCGTTGCATGTTCTGTGTTCCTGTAAATTCGTTGTTGTCGGATGCACGACCCGTAGGTCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTtgt                                                                                                                                                                                                                                                                                      <  2:290661/300‑1 (MQ=255)              tggCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGc                                                                                                                                                                                                                                                                                    <  1:772834/300‑1 (MQ=255)                           tccatGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGcaat                                                                                                                                                                                                                                                                       >  1:196959/5‑300 (MQ=255)                                     aaCCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAGATTCGGTGTTGTCGGATGCACGACCCGTAGGCAGGATAAGGCGCTCGCGCAGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCATGATAAGGCACTTGTGCCGCATCCGGCCATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGAATACAACTGTTGCAGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGa                                                                                                                                                                                                                                                             >  2:637901/1‑300 (MQ=18)                                     aaCCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGa                                                                                                                                                                                                                                                             >  1:3408/1‑300 (MQ=255)                                     aaCCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCATACAACTATTGCCGACCTGTCGGCCTGATAAGGCAATTGTGCCGCATCCGGCAATCAATGCCCGATACGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCAGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATTACTGCCTGa                                                                                                                                                                                                                                                             >  1:822151/1‑300 (MQ=21)                                                  tAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTTTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCATTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg                                                                                                                                                                                                                                                <  1:451261/300‑1 (MQ=255)                                                           gCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATc                                                                                                                                                                                                                                       <  1:520798/300‑1 (MQ=255)                                                           acATGTTCTGTGTTCCTGTAAATTCGTTGTTGTCGGATGCACGACCCGTAGGCCGGATAATGCACTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAANCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATNAATGCCTGATGCGACGCTGTCGCGTCTTATc                                                                                                                                                                                                                                       <  2:593942/299‑1 (MQ=255)                                                               gTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGTACGACCCGTAGACCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGAAGCTGTCGCATCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCAGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGACTACCACTGTTGCCGCCCCGTCGACCGGATAAGGCACTTGCGCCGCATCCGGCAAGTAACGCCTAAGACGACGCTGTCGCCTCTTCTCAGGc                                                                                                                                                                                                                                   >  2:27340/1‑300 (MQ=17)                                                               gTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGc                                                                                                                                                                                                                                   >  1:226199/1‑300 (MQ=255)                                                                ttCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGcc                                                                                                                                                                                                                                  >  1:30205/1‑300 (MQ=255)                                                                      gtTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAc                                                                                                                                                                                                                            <  1:19312/300‑1 (MQ=255)                                                                                 ttCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGAcc                                                                                                                                                                                                                 <  1:108328/300‑1 (MQ=255)                                                                                         tgtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGcc                                                                                                                                                                                                         <  1:48784/300‑1 (MQ=255)                                                                                                       cccGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGTGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCAGCATCCGGCAATCAATGCCTGCTGCGACGCTGTCGCGTATTATCAGGCCTACAACTATTGCCAAAATGTAGGCCGGATAAGGCATTtg                                                                                                                                                                                           >  2:270288/1‑300 (MQ=14)                                                                                                                 ggATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGAGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATcc                                                                                                                                                                                 >  1:719006/1‑300 (MQ=255)                                                                                                                              gcgcCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc                                                                                                                                                                    <  1:677538/300‑1 (MQ=255)                                                                                                                              gcgcCGCAGCCGGCAAGCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACGATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATACGGCAATCAATGCCTGATGCGACGCTGTCGCGTCGTATCAGGCCTACAACTGTGGACGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc                                                                                                                                                                    <  2:640275/300‑1 (MQ=14)                                                                                                                                   gcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAGTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGAACTGTAGGGCGGATAAGGCACTTGTGCCGCATCCGAAAATCAATGCCTGATg                                                                                                                                                               >  1:184692/1‑300 (MQ=255)                                                                                                                                   gcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATg                                                                                                                                                               >  1:184860/1‑300 (MQ=255)                                                                                                                                         ggCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCATACAACTATTGCCGACCTGTAGGCCTGATAATGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCTACGCTGTCGCGTCTTATCATGCCTACAACTATTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGc                                                                                                                                                         <  2:412048/300‑1 (MQ=255)                                                                                                                                             atcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTc                                                                                                                                                     >  1:504780/1‑300 (MQ=255)                                                                                                                                                       gATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCTACCTGTAGTCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATc                                                                                                                                           <  1:452147/300‑1 (MQ=255)                                                                                                                                                       gATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGAACTGTAGACCGGATACGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCCGTCGCGTCTTATAAGGAATACAACCACTACCAAACCGTAGTACAGATAGCNCACTTGTGAGGCATCCGGCAACCACCACCTGATGCGACGCTgccacgnattatc                                                                                                                                           >  2:681848/1‑288 (MQ=11)                                                                                                                                                                                ggCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGt                                                                                                                  >  1:146604/1‑300 (MQ=255)                                                                                                                                                                                            ttGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCCAAAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCACTGCCTGATGCGACGCTGTCGCGCCTTATCAAGCCTACAACTATTGCCGAATTGTAGGCCGGATAAAGCACTTGTGCCGCATCCGGCAACCAATGCCTGACGCGACGCTACCGAGTCTTATCAGGACTACAACTGtcgtataacagtaggccggataag                                                                                                      >  2:221868/1‑277 (MQ=14)                                                                                                                                                                                              tccgcccTGTATGCATGGTAATGCACTTATGCCGCATCCGTCAATAACTGCCTGCTTCGCCGCTGTCTCTTCCTATCAGTCCTACACCTGTTTCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATTCGACGCGGTCGCGTCTTATCAGGCCTACAACTAGTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATTCGGCAATCAGTGCCTGATGCGACGCTGTCGCGCCCTATCAGGCCTACAACTGTTACCGACCCGTGGGCCGGATAAGgc                                                                                                    <  2:347726/295‑1 (MQ=14)                                                                                                                                                                                              tccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGgc                                                                                                    <  1:731622/299‑1 (MQ=255)                                                                                                                                                                                                    cTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTcgc                                                                                              >  1:674130/1‑300 (MQ=255)                                                                                                                                                                                                                       cTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCAGGATAAGGCGCTCGCGCAGCATCCGGCAGTGttt                                                                           >  1:216261/1‑300 (MQ=255)                                                                                                                                                                                                                           tgCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCAGGATAAGGCGCTCGCGCAGCATCCGGCAGTGTTTAccc                                                                       >  1:109925/1‑300 (MQ=255)                                                                                                                                                                                                                            gCCGCATCCGGCAATCAATGCCTGATGCGACGCTTTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCGTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCcg                                                                      <  1:4885/300‑1 (MQ=255)                                                                                                                                                                                                                                    cGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACt                                                              >  1:636441/1‑300 (MQ=255)                                                                                                                                                                                                                                        actcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCaaa                                                          >  1:390768/3‑300 (MQ=255)                                                                                                                                                                                                                                                                      ttATCAGGCCTACAACTGTTGACGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACGAATGTTGCGGACCCGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACTCGCAACGACTCAAAATTTCTTtctcctcggcccacccgctctcc                            >  2:177296/1‑281 (MQ=17)                                                                                                                                                                                                                                                                       tctCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCAGAACTGTCGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGAAGCTGTCGCGTCTTATCAGGCCTACAAATGTTGACGAAACGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGTGGCGACTCAAAGTTTCCTTCTCATAAGCCCGCACGcgctaca                           >  2:426548/3‑294 (MQ=17)                                                                                                                                                                                                                                                                       tATCAGGCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGAACTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGAATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTCTCTCATAAGCCCGCACGctctaca                           >  2:113283/1‑297 (MQ=255)                                                                                                                                                                                                                                                                             gCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCAtt                     >  1:238308/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gCCTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCAtt                     >  2:51243/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               cTACAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCCATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTAGAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATTAATGCCTGCTGCGACGCTGTCGCTTCTTATCAGGCCTCCACCTGTTGCCGCCCCGTCAGCCGGGTAAGGCTCTCGCGTCGCATCCGGCCGTGTTTACCCGCGACGACTCCAAGGTTATTGCTCATAAGccctcccgttctacagacattac                   >  2:478350/1‑280 (MQ=14)                                                                                                                                                                                                                                                                                  cAACTGTTGCCGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGNAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGccctgtctcttatacacatctcgcag                <  1:568541/300‑24 (MQ=255)                                                                                                                                                                                                                                                                                            cGACCTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCg      <  1:727166/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               ccTGTAGACCGGATAAGGCACTTGTGCCGCCTCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAAGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTACCAAACAGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTCCCCGCGGCGACTACAAATTTCTTTCTCATAAGCCCTCACGCCCTACAGCCATTCACTACCTACTGGCgcat   >  2:71913/1‑297 (MQ=17)                                                                                                                                                                                                                                                                                               ccTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTACAGCCATTCGCTACCTGCTGGCGTAt   >  1:64280/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                cTGTAGACCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATc  >  1:100177/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      CAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATC  >  NC_000913/4094211‑4094797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑