Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,588	C→A	noncoding (35/85 nt)	tyrT ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,588	0	C	A	98.3%	205.7 / NA	60	noncoding (35/85 nt)	tyrT	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (31/28);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCG  >  NC_000913/1287304‑1287881                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                      gaatatttaattgcggattcgttgggaagttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccacccggggtaattcaacttttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggtcgttgcctgtggcaacgttctctcgctttcgctcgaatcgaacattagtcgaTGACGGCAGATTTAACGTCTGCTCCCTTTg                                                                                                                                                                                                                                                                                        >  2:396930‑M2/271‑300 (MQ=255) gaatatttaattgcggattcgttgggaagttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTg                                                                                                                                                                                                                                                                                        >  1:355230‑M2/271‑300 (MQ=255)   atatttaattgcggattcgttgggaagttcaggtacttttgaaattgatggtggtggtggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGc                                                                                                                                                                                                                                                                                      <  1:510647‑M2/32‑1 (MQ=255)   atatttaattgcggattcgttgggaagttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGc                                                                                                                                                                                                                                                                                      >  1:747098‑M2/269‑300 (MQ=255)   atattcaattgcggattcgtttggaagttcagggacttttaaaatggatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaatttttaggtaatgcttgagatggtggtggggtaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGc                                                                                                                                                                                                                                                                                      <  2:383846‑M2/32‑1 (MQ=255)   atattcaattgcggattcgttggtaagttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGc                                                                                                                                                                                                                                                                                      <  1:392797‑M2/32‑1 (MQ=255)                 attcgttgggaagttcagggacttttgaaagtgatggtggtggtggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAAcccc                                                                                                                                                                                                                                                                        <  2:165249‑M2/46‑1 (MQ=255)                     gttgggaagttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCccacca                                                                                                                                                                                                                                                                    >  1:154337‑M2/251‑300 (MQ=255)                      tttttaagttcagagacttttgaaagtgattgtgttggtggaagaatttgaaccttcgaagtcgattacggcagatttaccttcttccccctttggccgctcgggaaccccaccatgggtaattaagattttgagttagtgcttgagatggtggtgggggaaggattattcgtcgcttcgcgcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCTGGAACCCcaccac                                                                                                                                                                                                                                                                   <  1:257232‑M2/51‑1 (MQ=255)                      ttgggaagttcagggacttttgaaagtgatggtggtgggggaagggttcgaaccttcgaagtcgatgacggcagatttacagtctgctcccttttgccgctcgggaaccccaccaggggtagttcaaattttgaggtaatgcttgagatgttggtggggtaaggattattcgtcgtttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCcaccac                                                                                                                                                                                                                                                                   <  2:468437‑M2/51‑1 (MQ=255)                           gaggtcagggacgttggaagttgatggtgttgtgggaagggttcgaaccggcgaagtcgatgacggcagatctacagtctgctccctttggccgctcgggaacccnaccaggggtaattcaaattttgaggtaatgctttagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                                                                                                                                                                                                                                              <  2:706463‑M2/56‑1 (MQ=255)                             gttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtagtgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgtttcctatggcaccgttctctcgatttcgctcgaatcgaaccttagtcgaTGAAGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACcacggggcag                                                                                                                                                                                                                                                            >  2:728077‑M2/243‑297 (MQ=255)                             gttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTaa                                                                                                                                                                                                                                                            >  1:145542‑M2/243‑300 (MQ=255)                             gttcagggacttttgaaagtgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcaaccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTaa                                                                                                                                                                                                                                                            >  2:463698‑M2/243‑300 (MQ=255)                                cagggacttgggaaagtgatggtggtggtggaaggattcgaaccttcgaaagcgatgatggcagatttacagtctgctccctttggccactcgggaaccctaccaggggtgattcaaattttgaggtgatgcttgggatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaactttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGc                                                                                                                                                                                                                                                         <  2:764163‑M2/61‑1 (MQ=255)                                cagggacgtttgaaagtgatggtggtggtttaagtattcgaaccttctaattcgatgacggcagatttacattctgctcccctcgtccgctcgtgaaccccaccatgggtaattcaaatttttatttaatgctttagatggtggtgggggaaggagtattcgtcgctgcgcgcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGc                                                                                                                                                                                                                                                         <  1:92793‑M2/61‑1 (MQ=255)                                            aaagtggtggtggtggttgaaggattcgaaccgtcggggtcgatgacggcagatctacagtctgctccctttggccactcgggaaccccaccatgggtagttcaggttttgaggtagtgcttgagatggtggtggtggaaggattattcgtcgcttcgctcctcaccctgcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaacctgagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGcc                                                                                                                                                                                                                                             <  2:429312‑M2/73‑1 (MQ=255)                                                tgatggtggtgggggaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCt                                                                                                                                                                                                                                         >  1:467996‑M2/224‑300 (MQ=255)                                                         tggggtaaggattcgaaccttcgaagtcgatgacggcagatttacagtctgctcccttttgccgctcgggaaccccaccaggggtaattcaaattgtgaggtaatgcttgagatggtgttgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCg                                                                                                                                                                                                                                <  2:465725‑M2/86‑1 (MQ=255)                                                          ggggtaaggattcgaaccttcgaagtcgatgacggcagatttccagtctgctccctttggcagatcgggaacccaaccaggggtaattcaagtttgtaggtgatgctttagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgagtcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCgg                                                                                                                                                                                                                               <  1:285596‑M2/87‑1 (MQ=255)                                                                                  gtcgatgacggcagatttacagtctactccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAAt                                                                                                                                                                                                       <  2:726485‑M2/111‑1 (MQ=255)                                                                                               gatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcacattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgcatcgaaccttagtcgaTGACAGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGAGCCGCTGt                                                                                                                                                                                          >  2:264626‑M2/177‑300 (MQ=255)                                                                                               gatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaacnttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAGTGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGt                                                                                                                                                                                          >  2:195825‑M2/177‑300 (MQ=255)                                                                                               gatttacagtctgctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGt                                                                                                                                                                                          >  1:166754‑M2/177‑300 (MQ=255)                                                                                                   tacagtctgctccctgtggccgctctggaaccccgccagtggtaattccaattttgaggtgatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgcttncgctcgaatcgaaccttagtcgaTGANGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGTTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAg                                                                                                                                                                                      <  2:535020‑M2/128‑1 (MQ=255)                                                                                                           gctccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgctttagatggtggtgggggaaggattattcgtcgcttcgctcctaacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGt                                                                                                                                                                              <  1:778008‑M2/136‑1 (MQ=255)                                                                                                              ccctttggccgctcgggaaccccaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAAGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTga                                                                                                                                                                           >  2:437934‑M2/162‑300 (MQ=255)                                                                                                              ccctttggccgctcgggaaccccaccaggggnaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaangttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAACGAAGCGCAGCTGTAAAGTGTTAAGTTga                                                                                                                                                                           >  2:564017‑M2/162‑300 (MQ=255)                                                                                                              cccctcgaccgctcgggaaccccaccaagggtaattcaaattttgagataatgcttgagatggtggtaggggaaggattattcgtcgcttcgctcctcacccttagggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCAGGAACCCCACCACGGGGTAATGCTTTCCACTGGCCTGCTCCCCTATCGGGAAGCTGGGCGCATCATATCAAAtgacgcgccgctgtacaatatcacgctaa                                                                                                                                                                           >  2:342274‑M2/162‑286 (MQ=255)                                                                                                                         ctcgggaacccccccaggggtaattcaaattttgaggtaatgcttgagatggtggtggaggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGTTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGCGAAAAATGAAc                                                                                                                                                                >  2:19715‑M2/151‑300 (MQ=255)                                                                                                                                   caaccaggggtaattcaaattttgaggtaatgcttgagatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGAAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACTTTGAGACAAATGAACTGGTTGCGTa                                                                                                                                                      >  2:397833‑M2/141‑300 (MQ=255)                                                                                                                                                            ggtaatgcttgagatggtgttggtggaaggattattcgtcgcttcgctcctcactctttgggccgttgtctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCTGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAgg                                                                                                                             <  2:272345‑M2/185‑1 (MQ=255)                                                                                                                                                                        gatggtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAtt                                                                                                                 >  1:311579‑M2/104‑300 (MQ=255)                                                                                                                                                                            gtggtgggggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTg                                                                                                             >  1:442458‑M2/100‑300 (MQ=255)                                                                                                                                                                                   gggaaggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGa                                                                                                      >  1:799960‑M2/93‑300 (MQ=255)                                                                                                                                                                                        ggattattcgtcgcttcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCNGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCNTCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTACATCCGTAACGGATTAAAGGTAAACAGTTATTTTTGCTGGCGATTaaa                                                                                                 >  2:633657‑M2/88‑300 (MQ=255)                                                                                                                                                                                                       tcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGAt                                                                                  >  1:442158‑M2/73‑300 (MQ=255)                                                                                                                                                                                                       tcgctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAAAGGATTAAAGGTAAACAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGAt                                                                                  >  1:339562‑M2/73‑300 (MQ=255)                                                                                                                                                                                                         gctcctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTCATTTTCATCCGTCACGGATTAACGGTCACCAGTTATTTTTGATGGCGATTAAAGAATAATCGTTCGATTa                                                                                >  2:679826‑M2/71‑300 (MQ=255)                                                                                                                                                                                                            cctcacccttcgggccgttgcctgtggcaacnttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTANTGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGAGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCg                                                                             >  2:575673‑M2/68‑300 (MQ=255)                                                                                                                                                                                                            cctcacccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGCAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCg                                                                             >  2:140126‑M2/68‑300 (MQ=255)                                                                                                                                                                                                                 cccttcgggccgttgcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAAc                                                                        >  1:50093‑M2/63‑300 (MQ=255)                                                                                                                                                                                                                   cttcgggccgttgcctgtggcaacgttctctngctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTNTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACaa                                                                      >  2:563596‑M2/61‑300 (MQ=255)                                                                                                                                                                                                                               gcctgtggcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGTCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCtgtg                                                          >  1:377280‑M2/49‑300 (MQ=255)                                                                                                                                                                                                                                cctgtggcaacgttctctcgctggcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGc                                                         <  1:443023‑M2/253‑1 (MQ=255)                                                                                                                                                                                                                                      gcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAc                                                   <  1:1853‑M2/259‑1 (MQ=255)                                                                                                                                                                                                                                      gcaacgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACNGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAc                                                   <  1:576191‑M2/259‑1 (MQ=255)                                                                                                                                                                                                                                          cgttctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTg                                               >  1:319286‑M2/38‑300 (MQ=255)                                                                                                                                                                                                                                             tctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGtat                                            <  2:109934‑M2/266‑1 (MQ=255)                                                                                                                                                                                                                                             tctctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGtat                                            <  1:649684‑M2/266‑1 (MQ=255)                                                                                                                                                                                                                                               ttttgtgtgagcgcgcagaacacctgagtcgaGGACGGCAGAGTTAAAGTCTGCTCCCTTTGGCCGCTCTGGAACCCCACCAAGGGGTAATGCTTTTTACTGGCCTGCTCCCTNATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAANATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                          <  2:626819‑M2/268‑1 (MQ=255)                                                                                                                                                                                                                                               tctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGTCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                          <  2:373250‑M2/268‑1 (MQ=255)                                                                                                                                                                                                                                               tctcgctttcgctcgaatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTNCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                          <  1:544149‑M2/268‑1 (MQ=255)                                                                                                                                                                                                                                                       tcgctcgaatcgaaccttagtcaaTGACGGCAGATTTAAATTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGAc                                  <  1:595390‑M2/276‑1 (MQ=255)                                                                                                                                                                                                                                                              aatcggaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCACTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTATCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGAc                           <  2:50316‑M2/283‑1 (MQ=255)                                                                                                                                                                                                                                                              aatcgaaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGTGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGAc                           >  2:252219‑M2/18‑300 (MQ=255)                                                                                                                                                                                                                                                                   aaccttagtcgaTGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGtttt                      >  2:684159‑M2/13‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGAc       <  1:262971/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gCAGATTTAAATTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGAccc     <  1:265476/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                       gATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg  >  1:247592/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                      CCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCG  >  NC_000913/1287304‑1287881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑