Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,772	T→C	F236F (TTT→TTC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,772	0	T	C	100.0%	216.4 / NA	58	F236F (TTT→TTC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/35);  total (23/35)

GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGT  >  NC_000913/4396476‑4397050                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                       gTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCaaa                                                                                                                                                                                                                                                                                     <  1:511841/300‑1 (MQ=255)             cgcgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAAcc                                                                                                                                                                                                                                                                         <  2:615492/300‑1 (MQ=255)                                            gcggcAGCGAACCTGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGGGTTATCAGCAGTAATACGTTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGTTGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTAGCGCTATCGGCCCCGTTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAACGATGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATgg                                                                                                                                                                                                                                          <  2:15426/296‑1 (MQ=255)                                               tCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAACATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGgc                                                                                                                                                                                                                                       >  2:81108/1‑300 (MQ=255)                                                      aCCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGa                                                                                                                                                                                                                                <  1:42427/300‑1 (MQ=255)                                                        cGGCGCGTAGTCCGTTTAAAACGGAGAGTAACCGCACGACGGGTGTTATAAGCAGGAATACGGTGACGCATCCGGCAGCGCGCGCGACGGCCAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACTGCGGTCAGGGCCCTGGCGCTATCGGCCCCGGTGGTACGCGGGATAAAAATGTCACCATCGCCGGCGCGCGTAAATGGCGTACTTTGCTCAATTACGATCCGATGTTCAAGGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGAtg                                                                                                                                                                                                                              <  2:29546/300‑1 (MQ=255)                                                        cGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGAtg                                                                                                                                                                                                                              <  1:110932/300‑1 (MQ=255)                                                           cgcgcgATCCGTGTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCTCGACGGCTAACACTGGCGATAAGGTCATCATCGCTATTGATGCCGGACACGGCGATCAGGACCCTGGCGCTATCGGCCCCGTGGGTACGCCGGAGAAAAATGTCACCATCTCCAGCGCGCGTAAGTTGCATACTTTGCTCAATTACGATCCGATGTTCAAGGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTgg                                                                                                                                                                                                                           <  2:225203/300‑1 (MQ=255)                                                           cgcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTgg                                                                                                                                                                                                                           <  1:83901/300‑1 (MQ=255)                                                               cAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACg                                                                                                                                                                                                                       <  1:782644/300‑1 (MQ=255)                                                                    cGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGGAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGc                                                                                                                                                                                                                  <  1:264192/300‑1 (MQ=255)                                                                      tttAAAACGGAAAGTAACCGCACTACGGGTGCTATCAGCAGTAATACGGGAACGCGTCCGGCAGCGCGCGCGACGGCGAACACTGGCGATAAAATGATCATCGCTATGGATGCCGAACACGGCGGTCAGGACACTGGCGCTATCGGCCCCGGTGGGACGCGGGAGAAAAATGGCGACATCGCCATCGCGAGTAAATTGCGTACTTGGCTCAATGACGCTCCAATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTCATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCaa                                                                                                                                                                                                                <  2:160050/300‑1 (MQ=255)                                                                         aaaaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATAGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAc                                                                                                                                                                                                             <  1:67090/300‑1 (MQ=255)                                                                                      aCCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCg                                                                                                                                                                                                <  1:96132/300‑1 (MQ=255)                                                                                            cTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTGACCCGTGACGGGGATTACTTTATCTAGNTGATGGGGCGCAGTGATGTGGAACGTAGGCAAAAAGCCAATTTCCTCGTGTCGa                                                                                                                                                                                          >  2:585619/1‑300 (MQ=255)                                                                                            cTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGa                                                                                                                                                                                          >  1:375/1‑300 (MQ=255)                                                                                                 ggTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCAGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAATGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCAc                                                                                                                                                                                     >  2:510669/1‑300 (MQ=255)                                                                                                         cagcagTGATACGTTAGCGCGGCCGGCAGAGCGCGCGACGGCTAGCACTGGCGATAAAATTATCACCGCTATTGATGCCGGATACGGCGGGCAGGACCCTGGCGCGAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCTCCATCGCGCGTAAATTGCGTACGTTGCTCAATTACGATCCGATGGTCAAGGGCGTTTTAACCCGGGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                             <  2:254089/300‑1 (MQ=255)                                                                                                         cagcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                             <  1:62111/300‑1 (MQ=255)                                                                                                         cagcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                             <  1:762597/300‑1 (MQ=255)                                                                                                            cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGAGCGCCATGATGTGGCACGTAAGCAAAACGCAAATTTCCTCGTGTCGATTCACGCTGATGcagc                                                                                                                                                                          >  2:221363/1‑297 (MQ=255)                                                                                                            cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGc                                                                                                                                                                          >  1:409725/1‑300 (MQ=255)                                                                                                              gTAATACGGTAACGCGGCCGGCAGCGCGCGCGACGGCTAACACTGTCGAGAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCAc                                                                                                                                                                        <  1:193218/300‑1 (MQ=255)                                                                                                                   acggggacgtgTCCGGCAACACGCGCAACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCCATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCATCACCACCATCGCGCGTAAATTGCGTACTTTGCGCAATGACAATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATTGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTTGTGTCGATTCACGCTGATGCCGCATCGAAc                                                                                                                                                                   <  2:130227/290‑1 (MQ=255)                                                                                                                   aCGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAAc                                                                                                                                                                   >  1:373162/1‑300 (MQ=255)                                                                                                                           gcgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGc                                                                                                                                                           >  1:627263/1‑300 (MQ=255)                                                                                                                             gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGa                                                                                                                                                         <  1:61898/300‑1 (MQ=255)                                                                                                                                       gcgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGGGCAGTGATGTGGCACGTAAGCAAAACGACAATTTCTTCGTGTCGATTCACGCTGATGCCGAACCGAACCGCAGTGCGACTGGCGCTTc                                                                                                                                               >  2:254663/1‑300 (MQ=255)                                                                                                                                              aCGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATgg                                                                                                                                        <  1:677074/300‑1 (MQ=255)                                                                                                                                               tgtctaatccttacgagaagaTTAGCATCGCTATTGAGTCTGTACACGGAGATGAGGACCCATGCGCTATCGGCTCCGGTGATACGCGGGAGAGAAATTTCACCGCCGCCACCCCGCTTAGATCGCGTATTTTACCCAATGATGATCCAATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATTTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATggg                                                                                                                                       <  2:379159/280‑1 (MQ=255)                                                                                                                                               cGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATggg                                                                                                                                       <  1:649191/300‑1 (MQ=255)                                                                                                                                                ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGACAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAANCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGAACGTAGGCAAAATGCCAATTTTCCCGTGTAGATTAACGCTGATGTCGACCCGAACCGCAGTGTGGCTGGCGCTTACGTCTGGGt                                                                                                                                      >  2:187380/1‑300 (MQ=255)                                                                                                                                                ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCNCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                      >  1:553595/1‑300 (MQ=255)                                                                                                                                                       acTGGTGATAAACTTATCATCGCTATTGATTCTGGACACGGCGTTTAGGACCCTGACGCTATCGGCCCTGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCATAAATTGCGTACTTTACGCAATGACGATCCGGTGTTCAAGGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGTGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGctctct                                                                                                                               <  2:379920/300‑1 (MQ=255)                                                                                                                                                          tgCGATNAAATTATCACCGCTATTGATGCCGTACACTGCGTTCAGGACCCTGGCNCTATCATCCCCGGTGGTGCGCGGGAGAAAAATGTCACTATCGCCATCGCGTGTAAGTGTCGTACTGTGCTCAATGACGATACGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAc                                                                                                                            <  2:666832/299‑1 (MQ=255)                                                                                                                                                            cGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCg                                                                                                                          <  1:800757/300‑1 (MQ=255)                                                                                                                                                                          tcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGCCGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAATCGTCGCGACAACAGTg                                                                                                            >  1:322624/1‑300 (MQ=255)                                                                                                                                                                          tcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTCACCGTCGCGCCAACAGTg                                                                                                            >  2:613844/1‑300 (MQ=255)                                                                                                                                                                            gCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGACATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGaa                                                                                                          >  1:31341/1‑300 (MQ=255)                                                                                                                                                                             cTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGTCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAACGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGaaa                                                                                                         <  2:484058/300‑1 (MQ=255)                                                                                                                                                                                  gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGcc                                                                                                    <  1:66321/300‑1 (MQ=255)                                                                                                                                                                                               gcggttggtaCCCTGGCACTATCGGTCTCGGTGGTACGCGGTAGGGAAATGTCACCATCGCCATCGCGCGTAAATTGCATATTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAGCCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAGATGGCCAGCTGGCTGGagt                                                                                       <  2:349788/291‑2 (MQ=255)                                                                                                                                                                                                 ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcag                                                                                     >  1:342966/1‑300 (MQ=255)                                                                                                                                                                                                 ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCAAGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTCACCGTCGAGCCAACAGTGAAATGGCCAGCTGGCTGGggcag                                                                                     >  2:488442/1‑300 (MQ=255)                                                                                                                                                                                                    cAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCCGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCAc                                                                                  >  2:315463/1‑300 (MQ=255)                                                                                                                                                                                                       gACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACgag                                                                               <  1:31337/300‑1 (MQ=255)                                                                                                                                                                                                        aCCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACgaga                                                                              >  2:517824/1‑300 (MQ=255)                                                                                                                                                                                                                         cccGGTGTTACGCGGGAGAAAAATGTCACCATCGCCAGCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCTTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGANTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACATTGAAATGGCCAGCTGGCNGGAGCAGCACGAGAAACAGTCGGAGctgctg                                                             <  2:579908/300‑1 (MQ=255)                                                                                                                                                                                                                             gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGTGCCGTGATGTGGCATGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGTTCTCTAACCGTCGCGCCACCAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtg                                                         >  2:787376/1‑300 (MQ=255)                                                                                                                                                                                                                                 tgcgcgGGGGAAAAATTTCACCACCACCAGCGCACGTAAATCGCGTACTTTGCTCAATGACAATCCGATGTGCGAAGGCGTTTTAACCCTTGACGGGGATTACTTTATTTTGGTGAGGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGGGTCGATTCACGCTGATGTCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGc                                                     <  2:227543/298‑1 (MQ=255)                                                                                                                                                                                                                                                                  gcgTAAATTGCGTACTTTTCTCAACGACAATCCGATAGTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCACACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGTCGAACAGTCAGTCTGAccc                    <  2:135127/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  gcgTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc                    <  1:128208/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        atttcGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGAGGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTAttt              <  1:114282/296‑1 (MQ=255)                                                                                                                                                                                                                                                                        aTTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTAttt              <  1:114185/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aTTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGNCGATTCACGCTGATGCCGCACCGAACNGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTAttt              >  2:543879/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               aCTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCCGTGATATGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAACCCTATTTAAGCCAg       >  2:74021/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCCGTGCTGCGGCACGTAAGTAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTAACCGTCGAGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTAGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTACGCCAGGCGGt  >  2:52284/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGATCCATGATGTGGCACGTAAGCAAAACGACAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCGCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTAGGAGCTGCTGGGTGGGGCGGGTGATGTGTTGGCGAACAGTTAGTCTGCCCACTATTCACGCCAggtggt  >  2:52443/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                       GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGT  >  NC_000913/4396476‑4397050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑