Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,4110GA98.9% 332.5 / ‑4.7 91I28N (ATC→AAT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (46/44);  total (47/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
*NC_0009133,336,4120AT98.9% 318.1 / NA 92I28N (ATC→AAT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (46/44);  minor base G (0/1);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGTTTTTTAACCCGACTCATGCCGTCAAACAAC  >  NC_000913/3336119‑3336699
                                                                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                                                                
tgtgTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCa                                                                                                                                                                                                                                                                                           >  1:560833/1‑300 (MQ=255)
 gtgtAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCag                                                                                                                                                                                                                                                                                          >  1:89599/1‑300 (MQ=255)
         cAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCTCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCTGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCAt                                                                                                                                                                                                                                                                                  <  2:413389/300‑1 (MQ=255)
         cAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCAt                                                                                                                                                                                                                                                                                  <  1:279779/300‑1 (MQ=255)
               gACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAg                                                                                                                                                                                                                                                                            >  1:474639/1‑300 (MQ=255)
                                  aCCGCCAGGTAGTGAAACTTGCCCATGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTc                                                                                                                                                                                                                                                         >  2:317918/1‑300 (MQ=255)
                                   ccGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCg                                                                                                                                                                                                                                                        >  1:677909/1‑300 (MQ=255)
                                         ggTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATATAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTgg                                                                                                                                                                                                                                                  <  1:281474/300‑1 (MQ=255)
                                            aGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGNTGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTNAGCGCCGGAAATTGTGACTTCGCCCTGGAGc                                                                                                                                                                                                                                               <  2:527021/300‑1 (MQ=255)
                                            aGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGc                                                                                                                                                                                                                                               >  1:432986/1‑300 (MQ=255)
                                              tGAAACTGGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATATAAGCGCGCATGGTTGTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCTGGAAATTGTGACTTCGCCCTGGAGCtt                                                                                                                                                                                                                                             <  2:188757/300‑1 (MQ=255)
                                                 aaCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCNCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGNTT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGt                                                                                                                                                                                                                                          <  2:696598/300‑1 (MQ=255)
                                                        ccatGACCAAAGCGCGCTACCAGCGGTCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGcccc                                                                                                                                                                                                                                   <  2:281141/297‑1 (MQ=255)
                                                         ccTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACNTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCt                                                                                                                                                                                                                                  >  1:533944/1‑300 (MQ=255)
                                                           tGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGa                                                                                                                                                                                                                                >  1:325614/1‑300 (MQ=255)
                                                               cAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCGTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAacac                                                                                                                                                                                                                            <  2:673805/300‑1 (MQ=255)
                                                                                        cgcCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTtct                                                                                                                                                                                                   >  1:622922/1‑300 (MQ=255)
                                                                                            cAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGt                                                                                                                                                                                               <  2:396811/300‑1 (MQ=255)
                                                                                                   aaGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACTTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCNAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAANTTATCCATTTAGTTTGTTCTCAGTTAACAAt                                                                                                                                                                                        <  2:529291/300‑1 (MQ=255)
                                                                                                   aaGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCNGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAAt                                                                                                                                                                                        <  2:550998/300‑1 (MQ=255)
                                                                                                         gCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACNTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAANATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCatat                                                                                                                                                                                  >  2:587313/1‑300 (MQ=255)
                                                                                                          cATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAGAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATc                                                                                                                                                                                 <  1:43089/300‑1 (MQ=255)
                                                                                                              gTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCt                                                                                                                                                                             >  2:412056/1‑300 (MQ=255)
                                                                                                              gTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCt                                                                                                                                                                             >  1:732204/1‑300 (MQ=255)
                                                                                                              gTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGAACAGAACCATTAAGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTCACAATTCATATCCGCt                                                                                                                                                                             >  2:454653/1‑300 (MQ=255)
                                                                                                                        gATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTCGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAAt                                                                                                                                                                   >  2:441169/1‑300 (MQ=255)
                                                                                                                         aTCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAANTTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGNCGAATc                                                                                                                                                                  >  2:600459/1‑300 (MQ=255)
                                                                                                                         aTCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATc                                                                                                                                                                  >  1:647368/1‑300 (MQ=255)
                                                                                                                         aTCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGGTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATc                                                                                                                                                                  >  2:627936/1‑300 (MQ=255)
                                                                                                                                 gTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCTGCTACCGGCGAATCGCCCATAg                                                                                                                                                          >  2:117294/1‑300 (MQ=255)
                                                                                                                                        gAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGAAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCACTACCGGCGAATCGCCCATAACTCaaaa                                                                                                                                                   >  1:683140/1‑300 (MQ=255)
                                                                                                                                          aTACATTAACGTCGCGGGCATCAATATACACAAAACCGTTACGTTCTACTTTCGCACCCAGCTGACTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTTGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGc                                                                                                                                                 <  2:41893/300‑1 (MQ=255)
                                                                                                                                                         gggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCTGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGAt                                                                                                                                  <  1:712410/300‑1 (MQ=255)
                                                                                                                                                         gggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGAt                                                                                                                                  <  2:414335/300‑1 (MQ=255)
                                                                                                                                                            cATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATcgc                                                                                                                               >  1:414666/1‑300 (MQ=255)
                                                                                                                                                              tCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCCTAGCTCAAAAGCCGTTCAGTTTGCGATcgcgc                                                                                                                             >  1:335715/1‑300 (MQ=255)
                                                                                                                                                                actatGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCgcgcgc                                                                                                                           >  1:588660/3‑300 (MQ=255)
                                                                                                                                                                aaTATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCgcgcgc                                                                                                                           >  1:612981/1‑300 (MQ=255)
                                                                                                                                                                aaTATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCACTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCgcgcgc                                                                                                                           >  2:421621/1‑300 (MQ=255)
                                                                                                                                                                     gCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATNTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTACGCCAGTAGTGCGGCAAAA‑GGATTAGGCAGAGCAGCATTTTTCGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTNCCGGCGAATCGACCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACt                                                                                                                      >  2:680463/1‑300 (MQ=255)
                                                                                                                                                                          gAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCa                                                                                                                 >  1:96333/1‑300 (MQ=255)
                                                                                                                                                                            aCCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATCTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAgg                                                                                                               <  1:579458/300‑1 (MQ=255)
                                                                                                                                                                             ccATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAggg                                                                                                              >  1:160576/1‑300 (MQ=255)
                                                                                                                                                                               aTTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTCTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCCGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGGAGGGGt                                                                                                            >  2:626543/1‑300 (MQ=255)
                                                                                                                                                                                     ttCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGc                                                                                                      >  1:449944/1‑300 (MQ=255)
                                                                                                                                                                                     ttCTACTTTCGCACCAAGATGACGTAGCAACTTCATTGATGTATCGACGCCTTTCAGTTTCGCGACGTTCGGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGACAAAAAAATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGGGTTTCGTCGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCATTCAGTTTGCGATCCCGCGCCCACTCCGCAGGGGTATACGc                                                                                                      <  2:135562/300‑1 (MQ=255)
                                                                                                                                                                                      tCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCt                                                                                                     <  1:300425/300‑1 (MQ=255)
                                                                                                                                                                                      tCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCAGTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCt                                                                                                     <  2:622882/300‑1 (MQ=255)
                                                                                                                                                                                         aCTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTg                                                                                                  >  1:409207/1‑300 (MQ=255)
                                                                                                                                                                                               gCACCCCGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGac                                                                                            <  2:627675/300‑1 (MQ=255)
                                                                                                                                                                                                    cAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATAAGCTTTGATCGACACAGc                                                                                       >  2:425245/1‑300 (MQ=255)
                                                                                                                                                                                                      gCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCAt                                                                                     <  1:467104/300‑1 (MQ=255)
                                                                                                                                                                                                       cTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCTGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCTTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCTGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATg                                                                                    <  2:250324/300‑1 (MQ=255)
                                                                                                                                                                                                         ggCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGaa                                                                                  >  1:18706/1‑300 (MQ=255)
                                                                                                                                                                                                         ggCTTAGCAGCTTAATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCAAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATNTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACTGGCGAATCGCACATAGCTCAAAAGCAGTTTAGTTTGCGATCGCGTGCCCACTCCGCCGGGGTATACGCTCTGATCGCAACAGCATGaa                                                                                  >  2:171741/1‑300 (MQ=255)
                                                                                                                                                                                                           ctgagcagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGNCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCTGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATg                                                                                <  2:177742/297‑1 (MQ=255)
                                                                                                                                                                                                           cTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGGGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATg                                                                                <  2:321987/300‑1 (MQ=255)
                                                                                                                                                                                                           cTTAACAGCTTCATTGATGTATCGACTTCTTTCATTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTCCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATg                                                                                <  2:40386/300‑1 (MQ=255)
                                                                                                                                                                                                                ccgcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGtt                                                                           >  1:437550/3‑300 (MQ=255)
                                                                                                                                                                                                                cagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGtt                                                                           >  2:119575/1‑300 (MQ=255)
                                                                                                                                                                                                                  gcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTAt                                                                         >  1:378800/1‑300 (MQ=255)
                                                                                                                                                                                                                  gcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCACATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTAt                                                                         >  2:453504/1‑300 (MQ=255)
                                                                                                                                                                                                                    ttCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATcc                                                                       <  1:301799/300‑1 (MQ=255)
                                                                                                                                                                                                                     tCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCg                                                                      >  1:559088/1‑300 (MQ=255)
                                                                                                                                                                                                                      cATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGCTCTCTACCGGTTCTTCCGCCAGTAGTGCTGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGc                                                                     <  2:627097/300‑1 (MQ=255)
                                                                                                                                                                                                                            tGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAatata                                                               >  2:566395/1‑300 (MQ=255)
                                                                                                                                                                                                                              tATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATAtt                                                             <  2:407238/300‑1 (MQ=255)
                                                                                                                                                                                                                               aTCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTc                                                            >  1:40051/1‑300 (MQ=255)
                                                                                                                                                                                                                                  gACGTCTTTCAGTTTCTGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCAt                                                         <  2:426391/300‑1 (MQ=255)
                                                                                                                                                                                                                                  gACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCAt                                                         <  1:726880/300‑1 (MQ=255)
                                                                                                                                                                                                                                      tCTTTCAGTTTCGGGACGTTCTGGATTTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCCCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGc                                                     >  2:358651/1‑300 (MQ=255)
                                                                                                                                                                                                                                             gTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCAt                                              <  1:430377/300‑1 (MQ=255)
                                                                                                                                                                                                                                             gTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGAGT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACGTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCTGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCAt                                              <  1:737968/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 ctggACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAc                                          <  2:565487/298‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTNGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAc                                          <  1:524739/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAc                                          <  1:338440/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAc                                          <  2:122438/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGGGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAc                                          <  1:25435/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGGAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTAGACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAc                                          <  1:26076/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGGACATTCTGGATCTTTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTTGAGCTTCGTTTGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGTACCATAGAc                                          <  2:320645/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  tggACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAcc                                         <  1:712077/299‑1 (MQ=255)
                                                                                                                                                                                                                                                  ggggcGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAcc                                         <  2:383361/296‑1 (MQ=255)
                                                                                                                                                                                                                                                  gggACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGAcc                                         <  1:116765/300‑1 (MQ=255)
                                                                                                                                                                                                                                                       gTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTctg                                    >  1:332180/1‑300 (MQ=255)
                                                                                                                                                                                                                                                        ttCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTctgc                                   >  1:386984/1‑300 (MQ=255)
                                                                                                                                                                                                                                                        ttCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCAATCAGCGGACCATAGACCGTctgc                                   >  2:195566/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGttt                              >  1:255081/1‑300 (MQ=255)
                                                                                                                                                                                                                                                               tctcTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTCTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGttttt                            >  2:572034/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                        gTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACTGTCTGCTGTTTTTTAACACGAc                   >  2:211217/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                          tcttcCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGTTTTTTAACCCGACTc                 <  1:171890/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      agtGCGGCAAAAAGATT‑GGGCAGAGCAGCACTTTCAGCGCCGGAAATTGTGACTTCGCCCTGTAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGTCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGTTTTTTAACCCGACTCATGCCGTCAaac     <  2:359614/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         gCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGTTTTTTAACCCGACTCATGCCGTCAaacaac  <  2:393446/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                                                                
TGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGACCATAGACCGTCTGCTGTTTTTTAACCCGACTCATGCCGTCAAACAAC  >  NC_000913/3336119‑3336699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑