Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,753	2 bp→CC	coding (1552‑1553/3075 nt)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,753	0	T	C	100.0%	200.0 / NA	54	K518G (AAA→GGA)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/31);  total (23/31)
*	NC_000913	364,754	0	T	C	100.0%	197.6 / NA	55	K518G (AAA→GGA)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/31);  total (24/31)

ATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGAT  >  NC_000913/364461‑365017                                                                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                                        aTGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGcac                                                                                                                                                                                                                                                                   <  1:633947/300‑1 (MQ=255)            ggTCGGCACAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGCAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCAGGGAAgg                                                                                                                                                                                                                                                        >  1:66330/1‑300 (MQ=255)            ggTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAgg                                                                                                                                                                                                                                                        >  1:794169/1‑300 (MQ=255)             gTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAggg                                                                                                                                                                                                                                                       >  1:395601/1‑300 (MQ=255)                 gCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTgg                                                                                                                                                                                                                                                   >  1:227973/1‑300 (MQ=255)                   aaaGACNAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCNCCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTc                                                                                                                                                                                                                                                 <  2:654689/300‑1 (MQ=255)                   aaaGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTc                                                                                                                                                                                                                                                 <  2:446451/300‑1 (MQ=255)                      gaccagaccGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTc                                                                                                                                                                                                                                              <  2:391372/300‑1 (MQ=255)                         cagaccGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCNAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATc                                                                                                                                                                                                                                           <  1:555538/300‑1 (MQ=255)                               gctCATACAGAACTGGCGATCGTTCAGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCGGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGCCGAAACGCCTGCCAGTATTTAGCGAAACCGACAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGAATCAGCGGGCGCGTCTCTCAAGGTAGCGAAAACCATTTTTTGATGGACCATCCAGGCACAGCCGGGAAGGACTGGTCTTAATCCAcgcg                                                                                                                                                                                                                                     >  2:246930/3‑300 (MQ=255)                                        gAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTATAAAGGTAGCGAAAGCCATTTTTTGATGGACCATCCAGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACAt                                                                                                                                                                                                                            >  1:309651/1‑300 (MQ=255)                                        gAACTGGCGATCGTTCGGCGTATCGCCAAAATCAACGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGCAGGGCTGGTCTTCATCCACGCGCGTGTACAt                                                                                                                                                                                                                            >  2:4647/1‑300 (MQ=255)                                        gAACTGGCGATCATTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCATTCCCAGACGAAGCCGCCCTTTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGACTGGTTTTCATCCACGCGCGCGTACAt                                                                                                                                                                                                                            <  2:28796/300‑1 (MQ=255)                                        gAACTAGCGATCGTTCGGCGGATCGCCAAAGCCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGTCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACAt                                                                                                                                                                                                                            <  2:213348/300‑1 (MQ=255)                                                   cGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGTGCAaataa                                                                                                                                                                                                                 <  1:795084/300‑1 (MQ=255)                                                         gCGTATCGCCAAAATCACCGCCGTGAGCCAACCACGGGTTGCCGTTTTCATCATATTTAATCAGCTACTGATCCACCCAGTCCCAGACGAAGTCGCCCTGTAAGCGTGGATACTGACTAAACGCCTGCCAGTATTTAGCGAAACCGCCAGCACTGTTACCCATCGCGTGGGCGTATGCGCAAAGGATCAGCGGACGCCCCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCTGCACAGCCGGGAAGGGCTAGTCCTCATCCACGCGCGCATACATCGGGCAaataccacccc                                                                                                                                                                                                           <  2:100166/300‑8 (MQ=255)                                                                     aaTCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTNGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGNCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGgtgt                                                                                                                                                                                               >  2:629259/1‑300 (MQ=255)                                                                          ccgccgTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCt                                                                                                                                                                                          <  1:512183/300‑1 (MQ=255)                                                                                         cactggTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATAc                                                                                                                                                                           <  1:147334/296‑1 (MQ=255)                                                                                         cACGGGTTGCTGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCTGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATAc                                                                                                                                                                           <  2:163766/300‑1 (MQ=255)                                                                                         cACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATAc                                                                                                                                                                           <  2:516802/300‑1 (MQ=255)                                                                                         cACGGGTTGCCGTTGTCATCATATGTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGANACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATAc                                                                                                                                                                           <  1:569232/300‑1 (MQ=255)                                                                                         cACGGGGTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATAc                                                                                                                                                                           <  2:731927/300‑1 (MQ=255)                                                                                          aCGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACt                                                                                                                                                                          <  1:212652/300‑1 (MQ=255)                                                                                              gTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAGAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCAc                                                                                                                                                                      <  1:795847/300‑1 (MQ=255)                                                                                              gTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCAc                                                                                                                                                                      <  1:81386/300‑1 (MQ=255)                                                                                                        tcatcaTATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGaa                                                                                                                                                            <  1:123110/300‑1 (MQ=255)                                                                                                                              gATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGc                                                                                                                                      >  1:158902/1‑300 (MQ=255)                                                                                                                                   aCCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATAc                                                                                                                                 >  1:717337/1‑300 (MQ=255)                                                                                                                                   aCCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTAACCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTACAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTCCATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCAACAGATTTGATCCAGCGATAc                                                                                                                                 >  2:350972/1‑300 (MQ=255)                                                                                                                                        gTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCATGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAgcgc                                                                                                                            >  2:349057/1‑300 (MQ=255)                                                                                                                                              gACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTNGCGAAAGCCATTTTTTGATGGACCATNCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTg                                                                                                                      >  2:522637/1‑300 (MQ=255)                                                                                                                                                 gAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCNTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGAtt                                                                                                                   <  1:533235/300‑1 (MQ=255)                                                                                                                                                      cgccCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAgcgc                                                                                                              <  1:73486/300‑1 (MQ=255)                                                                                                                                                        cacTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTATCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCg                                                                                                            >  2:95632/3‑300 (MQ=255)                                                                                                                                                          cTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTg                                                                                                          >  1:783512/1‑300 (MQ=255)                                                                                                                                                                       tACTGACGAAACGCCTGCCAGTAGGTAGCGAAACCGCCAAGACTGTTACCCATCGCGTAGGCGTATTCGCAAAGGATCAACGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGTACCATCCCGGCNCAGCCTGGAAGGGCTGGTCTGCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCTTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCTGGAAGGATCTACAGATTTGATCCAGCGATACAGTGCGTCGTGATTAGCGCCGTGGCCTGattcattc                                                                                             <  2:199316/300‑1 (MQ=255)                                                                                                                                                                                    ccTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAga                                                                                >  1:202677/1‑300 (MQ=255)                                                                                                                                                                                        ccAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAgatgat                                                                            >  1:782410/1‑300 (MQ=255)                                                                                                                                                                                             aTTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTANATCGGGCAAATAATATCGGTGGCCGTNGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCacac                                                                       <  2:547103/300‑1 (MQ=255)                                                                                                                                                                                                    gAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTg                                                                <  2:361088/300‑1 (MQ=255)                                                                                                                                                                                                         cGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAANGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTAc                                                           <  1:578896/300‑1 (MQ=255)                                                                                                                                                                                                            cAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTNTCCAGGTAGCGAAAGCCATTTTTTGATTGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGAt                                                        <  2:604895/300‑1 (MQ=255)                                                                                                                                                                                                               gACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGAACAGATGATCACACTCGGGTGATTACGATcgc                                                     >  1:424373/1‑300 (MQ=255)                                                                                                                                                                                                                   gTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTATCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGAACAGATGATCACACTCGGGTGATTACGATCGCGCTg                                                 >  1:224752/1‑300 (MQ=255)                                                                                                                                                                                                                                   gCGTATTCGCAAAGGATCAGCATGCGCGTCTCTCCAGGTAGCGAAGGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGTTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTAcg                                 <  2:369792/300‑1 (MQ=255)                                                                                                                                                                                                                                       aTTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCCCACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGtt                             >  1:647084/1‑300 (MQ=255)                                                                                                                                                                                                                                              aaGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCAcctgtctcttatacacatctcgcag                      <  1:693625/300‑24 (MQ=255)                                                                                                                                                                                                                                                         ggCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCTGGGAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGTTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGcc           <  2:141370/300‑1 (MQ=255)                                                                                                                                                                                                                                                         ggCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGNCGGGAAGGATCGACAGATTTGATCCANCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGcc           <  2:532898/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cgTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAgcg       >  1:657672/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGGTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGTGTCGTGATTAGCGCCGTGGCATGATTCATTCCCAAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTAAGCGTTCGCTCATTGCCGGTAGCCAGCGCGGa   >  2:722651/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCNTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGANTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGa   >  2:636240/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGa   >  2:259069/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  ctcCAGGTAGCGAAAGCCATTTTTTGATGGACCATCCCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGTGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCATTCGCTCATCGCCGGTAGCCAGCGCGGat  <  2:240828/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                                        ATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGAT  >  NC_000913/364461‑365017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑