| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,050,039 | A→C | T69T (ACA→ACC) S5R (AGC→CGC) |
insA4 → insB4 → |
IS1 family protein InsA IS1 family protein InsB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,050,039 | 0 | A | C | 92.3% | 23.4 / ‑2.6 | 13 | coding (207/276 nt) coding (13/504 nt) | insA4 insB4 | IS1 family protein InsA IS1 family protein InsB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (10/2); minor base . (1/0); total (11/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
CTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACAGCCCGCATTATGGGCGTTGGCCTCAACACGATT > NC_000913/1049747‑1050072 | cTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCa > 1:816280/1‑300 (MQ=255) ccTTTATTCCTCGCCTGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAAGTTCTCCAGTGGCTTCTGTTTCTCTCCGCTGTCCCTCCTTTTCAGCTACTGACGAGGTGGTGCGTAGTGGCAAAAGCACTGCCGGCCATTAGCGCTCTCCCTGCTCTCACTGCCGTAACACNTGGCACCTACAGTTCACTTACACCGCCTATAAACCCGGTACGCACCAGAAAAGGATTGATCTGGCCGTGAATGGCGTTGGATGCCGGGC‑AACCGCACGCATTATg > 2:824336/1‑300 (MQ=11) tttATTCATCGCATGGACAATACGGGTGAGGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATggg < 2:516856/300‑1 (MQ=255) tATTCATCGCATGGACAATACGGTTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTTAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCg < 2:251658/300‑1 (MQ=255) gCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCACCCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGTGTTGGATGTCGGGC‑AAACGCCCGCATCATGGGCGTTGGCCTc > 2:204503/1‑300 (MQ=255) ggACCATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGCGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAAACAGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCAGCATTATGGGCGTTGGCCTCAaca > 1:774011/1‑300 (MQ=255) ggACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGGGGTTCTACAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGACGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGTCGTTCACTTACACCGATTCTCAACCCGGCACGCACCAGAAAATCATTGATATGGCAACCAATGGCGTTGGATGCAGGGCCAAC‑GCACGCACTATGGGCGTTggtataaaca > 2:338286/1‑292 (MQ=11) ggACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAANGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACNGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCGTTGGCCTCAaca > 2:765868/1‑300 (MQ=18) ggACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCANTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCGTTGGCCTCAaca > 1:672302/1‑300 (MQ=18) ggACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCGTTGGCCTCAaca > 1:785579/1‑300 (MQ=18) ggACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACNGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCGTTGGCCTCAaca > 2:239198/1‑300 (MQ=18) cAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCGTTGGCCTCAACACGa > 1:336688/1‑300 (MQ=17) aTACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGACTGGCGTTGGATGCCGGGC‑AACCGCCCGCATTATGGGCGTTGGCCTCAACACGAtt > 1:291190/1‑300 (MQ=14) | CTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGC‑AACAGCCCGCATTATGGGCGTTGGCCTCAACACGATT > NC_000913/1049747‑1050072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |