Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,543,590	G→A	R159H (CGT→CAT)	fimA →	type 1 fimbriae major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,543,590	0	G	A	100.0%	290.7 / NA	84	R159H (CGT→CAT)	fimA	type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (64/20);  total (64/20)

GTACCGCATCGCTGGCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAAC  >  NC_000913/4543296‑4543878                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  gTACCGCATCGCTGGCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCattatt                                                                                                                                                                                                                                                                                              >  1:48919/1‑300 (MQ=255)          cGCTGGCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCANACCAACGTTCTGGCTCTGCAGAGTTCNGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCg                                                                                                                                                                                                                                                                                     >  2:500985/1‑300 (MQ=255)               gCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACANAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGcc                                                                                                                                                                                                                                                                                >  1:508489/1‑300 (MQ=255)               gCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAAAAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCAGTTCCAGGCGCATTATTTTGCAACCGGGGcc                                                                                                                                                                                                                                                                                >  1:332652/1‑300 (MQ=255)                      aaggaGCAACCATTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACTGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAAccc                                                                                                                                                                                                                                                                         <  1:177019/300‑1 (MQ=255)                      aaggaGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGACTGATTGCGATACCAATGTTGCATCGAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTACGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGTCGCAAccc                                                                                                                                                                                                                                                                         <  2:113592/300‑1 (MQ=255)                      aaggaGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAAccc                                                                                                                                                                                                                                                                         <  1:680631/300‑1 (MQ=255)                           gCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGt                                                                                                                                                                                                                                                                    <  1:391588/300‑1 (MQ=255)                             aaCCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTCCCAGGCGCATTATTTTGCAAACGGGGCCGCAACCCCGGGtgc                                                                                                                                                                                                                                                                  >  2:152728/1‑300 (MQ=255)                                  gTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACNCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTa                                                                                                                                                                                                                                                             >  1:498862/1‑300 (MQ=255)                                  gTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTa                                                                                                                                                                                                                                                             >  1:158822/1‑300 (MQ=255)                                       gctgTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCg                                                                                                                                                                                                                                                        <  2:719892/300‑1 (MQ=255)                                                        tCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTNGGTACGGCGATTGATGCGGGTCATACCGACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGt                                                                                                                                                                                                                                       <  2:552466/300‑1 (MQ=255)                                                         cAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGtt                                                                                                                                                                                                                                      >  2:713695/1‑300 (MQ=255)                                                          aGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTc                                                                                                                                                                                                                                     <  1:121963/300‑1 (MQ=255)                                                             tGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGt                                                                                                                                                                                                                                  <  1:392567/300‑1 (MQ=255)                                                               aaTGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAt                                                                                                                                                                                                                                <  1:399321/300‑1 (MQ=255)                                                                      gCGATACCAATTTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAc                                                                                                                                                                                                                         <  1:689073/300‑1 (MQ=255)                                                                          tACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAc                                                                                                                                                                                                                     >  1:135252/1‑300 (MQ=255)                                                                             cAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCa                                                                                                                                                                                                                  >  1:103105/1‑300 (MQ=255)                                                                               aTGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGNACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCACTAACCTACCCAgg                                                                                                                                                                                                                >  1:520715/1‑300 (MQ=255)                                                                               aTGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAgg                                                                                                                                                                                                                >  2:721111/1‑300 (MQ=255)                                                                               aTGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAgg                                                                                                                                                                                                                <  1:265845/300‑1 (MQ=255)                                                                               aTGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGAAATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAgg                                                                                                                                                                                                                >  1:369730/1‑300 (MQ=255)                                                                                 gTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTGTTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGGTGCGACCTTCAAGGTTCAGTATCAATAACCTAACCAGGtt                                                                                                                                                                                                              >  2:641279/1‑300 (MQ=255)                                                                                      aTCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAACCAGGTTCAggg                                                                                                                                                                                                         >  2:626980/1‑300 (MQ=255)                                                                                      aTCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGATGGATGGTGCGGCATTTAGTTCAGAAACAACCATGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAAACGGGGCAGCAACCCCGGGTGCTGCTAATGCGGATGCGAACTTCAAGGTTCAGTATCAATAACCTACACAGGTTCAggg                                                                                                                                                                                                         >  2:271650/1‑300 (MQ=255)                                                                                      aTCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGNTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGNTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAggg                                                                                                                                                                                                         >  2:687038/1‑300 (MQ=255)                                                                                      aTCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTAATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAggg                                                                                                                                                                                                         >  2:451230/1‑300 (MQ=255)                                                                                                           tttAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCAGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAAGTCATTACGGGCAGGGATg                                                                                                                                                                                    >  2:374258/1‑300 (MQ=255)                                                                                                                gTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCAc                                                                                                                                                                               <  1:419569/300‑1 (MQ=255)                                                                                                                   cGGCGATTGATGCGGGTCATGCCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGGAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCt                                                                                                                                                                            <  2:349868/300‑1 (MQ=255)                                                                                                                      cGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTtgt                                                                                                                                                                         >  1:633167/1‑300 (MQ=255)                                                                                                                            aTGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAANCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATa                                                                                                                                                                   >  2:554971/1‑300 (MQ=255)                                                                                                                            aTGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATa                                                                                                                                                                   >  2:629757/1‑300 (MQ=255)                                                                                                                            aTGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAACCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATa                                                                                                                                                                   >  2:442375/1‑300 (MQ=255)                                                                                                                                gggTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACTCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCAAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCAACCTTGTGCGATaaaaa                                                                                                                                                               >  2:54500/1‑300 (MQ=255)                                                                                                                                 ggTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAGCTTCAAGGTTCAGTATCAATAACCTACNCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAAt                                                                                                                                                              >  2:553003/1‑300 (MQ=255)                                                                                                                                 ggTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAAt                                                                                                                                                              >  1:297070/1‑300 (MQ=255)                                                                                                                                 ggTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCAGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAAt                                                                                                                                                              >  2:236406/1‑300 (MQ=255)                                                                                                                                     aTACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACg                                                                                                                                                          >  1:137602/1‑300 (MQ=255)                                                                                                                                            cGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGaaaa                                                                                                                                                   >  2:706500/1‑300 (MQ=255)                                                                                                                                              ttCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAgg                                                                                                                                                 >  2:719906/1‑300 (MQ=255)                                                                                                                                                  ggCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑aga                                                                                                                                            >  1:35321/1‑300 (MQ=255)                                                                                                                                                           gagTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGNCCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTc                                                                                                                                   >  1:499995/1‑300 (MQ=255)                                                                                                                                                           gagTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCAGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTc                                                                                                                                   >  2:415680/1‑300 (MQ=255)                                                                                                                                                             gTTCAGCTGCGGGTAGCGCAACAAACGCTAGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGTATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAACCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTCGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATGTCTa                                                                                                                                 >  1:165801/1‑300 (MQ=255)                                                                                                                                                                                      cGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGAACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAACATAACGATGAAAGGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTg                                                                                                        >  2:157261/1‑300 (MQ=255)                                                                                                                                                                                             gtgCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGc                                                                                                 >  1:360720/1‑300 (MQ=255)                                                                                                                                                                                             gtgCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTACTTCTATTAGCGTCGTTGCTGCCAACGTTTGCTCTGGc                                                                                                 >  2:11021/1‑300 (MQ=255)                                                                                                                                                                                             gtgCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTAAGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTgga                                                                                                 >  2:287935/1‑299 (MQ=255)                                                                                                                                                                                                   aTCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAANCCCGGGTGCTGCTAATGCGGATGCGANCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaa                                                                                           >  2:501964/1‑300 (MQ=255)                                                                                                                                                                                                   aTCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaa                                                                                           >  1:430033/1‑300 (MQ=255)                                                                                                                                                                                                        ggACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaat                                                                                      >  2:426507/1‑300 (MQ=255)                                                                                                                                                                                                        ggACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaat                                                                                      >  1:663713/1‑300 (MQ=255)                                                                                                                                                                                                        ggACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTATATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaat                                                                                      >  2:380549/1‑300 (MQ=255)                                                                                                                                                                                                             gAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAAt                                                                                 >  1:717686/1‑300 (MQ=255)                                                                                                                                                                                                             gAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAAt                                                                                 >  2:366091/1‑300 (MQ=255)                                                                                                                                                                                                                    tgctgcGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGt                                                                          <  1:232720/300‑1 (MQ=255)                                                                                                                                                                                                                        gcgcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATNCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGcc                                                                      >  1:496358/1‑300 (MQ=255)                                                                                                                                                                                                                        gcgcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGcc                                                                      >  1:293395/1‑300 (MQ=255)                                                                                                                                                                                                                        gcgcTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTATATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCCCGTTGcc                                                                      >  2:292012/1‑300 (MQ=255)                                                                                                                                                                                                                             gACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCcggcg                                                                 >  1:172579/1‑300 (MQ=255)                                                                                                                                                                                                                              aCGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTGTTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAAGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCACTGTTTGCTCTGGCCGGAACTAAATGGCATACCAAGTTGCCcggcgg                                                                >  2:216047/1‑300 (MQ=255)                                                                                                                                                                                                                              aCGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCcggcgg                                                                >  1:304016/1‑300 (MQ=255)                                                                                                                                                                                                                              aCGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTACTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCcggcgg                                                                <  1:47227/300‑1 (MQ=255)                                                                                                                                                                                                                                   ggATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAata                                                           >  2:436321/1‑300 (MQ=255)                                                                                                                                                                                                                                        gTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCaa                                                      >  1:437682/1‑300 (MQ=255)                                                                                                                                                                                                                                        gTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCAGGCAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCaa                                                      >  2:463855/1‑300 (MQ=255)                                                                                                                                                                                                                                        gTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTCTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCaa                                                      >  1:728389/1‑300 (MQ=255)                                                                                                                                                                                                                                        gTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTACAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCaa                                                      >  2:3105/1‑300 (MQ=255)                                                                                                                                                                                                                                              cATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAg                                                >  1:298672/1‑300 (MQ=255)                                                                                                                                                                                                                                                gttAGTGCATAAACAACCCTGAATAACGGAACCAATACCATTCCGTGCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTNCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAggg                                              <  1:503157/299‑1 (MQ=255)                                                                                                                                                                                                                                                         gAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCattat                                     <  1:137331/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAAATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCattat                                     <  1:137062/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaaCAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCattatt                                    <  1:318386/300‑1 (MQ=255)                                                                                                                                                                                                                                                               acCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGa                               >  1:54039/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gAACCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAACGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGACACTCGCCAGAtt                  >  1:400971/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             aaCCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTg                 >  1:749830/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aCCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGa                >  1:475974/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aCCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCACGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGa                >  2:298809/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aCCAATACCATTCCGTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCAGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGa                >  2:407331/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aCCAATACCATTCAGTCCCAGGTGCATTATTTCGCAACCGGGGCCGCAACCCCGAGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCCAGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAA‑GGACAGAGATTATTTCTATTAGTGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATCAATGGAATACTCCGTTGCATGGCGGAAATATGCAATTTAAAGGCGTCATTATTGCGGAAACTTGCCGGATTGa                >  2:7116/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAAc  <  1:176469/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gTTCCAGGCGCATTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGCTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAAc  >  1:634163/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  GTACCGCATCGCTGGCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCAGAGTTCAGCTGCGGGTAGCGCAACAAACGTTGGTGTGCAGATCCTGGACAGAACGGGTGCTGCGCTGACGCTGGATGGTGCGACATTTAGTTCAGAAACAACCCTGAATAACGGAACCAATACCATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGA‑AGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAAC  >  NC_000913/4543296‑4543878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑