Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,459,147	G→A	V397I (GTA→ATA)	tldE →	metalloprotease subunit TldE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,459,147	0	G	A	100.0%	308.5 / NA	87	V397I (GTA→ATA)	tldE	metalloprotease subunit TldE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/53);  total (34/53)

AAGGGCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGGTAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG  >  NC_000913/4458854‑4459441                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       aaGGGCTTGCGTCGACGCCATTCGACAGCGAAGGTATGCGCACCGAGCGTCGCGATATTATTAAAGATTGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGTTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAgaga                                                                                                                                                                                                                                                                                                  <  2:213750/300‑1 (MQ=255) aaGGGCTGTCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAgaga                                                                                                                                                                                                                                                                                                  <  2:389345/300‑1 (MQ=255) aaGGGCTGTCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATNCACAACTGGCGGATTGCCGGACAAGGNCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAgaga                                                                                                                                                                                                                                                                                                  <  2:501032/300‑1 (MQ=255) aaGGGCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGNAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAgaga                                                                                                                                                                                                                                                                                                  <  1:542937/300‑1 (MQ=255) aaGGGCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAgaga                                                                                                                                                                                                                                                                                                  <  1:663724/300‑1 (MQ=255) aaGGGCTGGCGTCGACGCCATTCGACAGCGAAGGTATGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTACCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGGATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAgaga                                                                                                                                                                                                                                                                                                  <  2:129177/300‑1 (MQ=255)   gggCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTAACGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAAt                                                                                                                                                                                                                                                                                                >  2:144868/1‑300 (MQ=255)    ggCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATg                                                                                                                                                                                                                                                                                               >  2:48964/1‑300 (MQ=255)             cGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTc                                                                                                                                                                                                                                                                                      <  1:284553/300‑1 (MQ=255)                cGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGt                                                                                                                                                                                                                                                                                   <  1:132043/300‑1 (MQ=255)                    aTTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATcc                                                                                                                                                                                                                                                                               <  1:744262/300‑1 (MQ=255)                      tCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTTGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCgg                                                                                                                                                                                                                                                                             <  1:120085/300‑1 (MQ=255)                          cAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAg                                                                                                                                                                                                                                                                         >  1:744728/1‑300 (MQ=255)                          cAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCAAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTCTCCGGTGAg                                                                                                                                                                                                                                                                         >  2:359264/1‑300 (MQ=255)                               aaGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGaaa                                                                                                                                                                                                                                                                    >  1:691007/1‑300 (MQ=255)                                                                aaGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGgc                                                                                                                                                                                                                                   <  2:453155/300‑1 (MQ=255)                                                                        aTCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGNCAGGGCGTGAGTGCCATTACCGGCGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATAtt                                                                                                                                                                                                                           >  2:187853/1‑300 (MQ=255)                                                                            tGACTCATTAGCTGCGGACCAGCTGCTCGGCGCGGAAACGGGGGCTGAGAAGCACCGGACATGCGGGCGGTATTTACAATTGGCGGATTGCCGGACAGGGTCTAAGCTTCGAGCAGATGCGCAAAGGGAGGGGCACCGGGCTGGTGGTGACGGACTTGATGGGCCATGGCGTGAGTGCCATTACCGGTAATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATGTAAAAGATATGTGGCGCAATATTGTCa                                                                                                                                                                                                                       <  2:82383/300‑1 (MQ=255)                                                                             gACTCANTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACNGGACATGCGTGCGGTATTCACAACTGTCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGTTGGTGCCGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCAc                                                                                                                                                                                                                      <  2:618682/300‑1 (MQ=255)                                                                               cTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCg                                                                                                                                                                                                                    >  1:601653/1‑300 (MQ=255)                                                                                       ctgctgACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCAACGTCGGTAAc                                                                                                                                                                                                            >  1:154223/1‑300 (MQ=255)                                                                                         gctgACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGa                                                                                                                                                                                                          >  1:305950/1‑300 (MQ=255)                                                                                         gctgACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGa                                                                                                                                                                                                          >  2:310291/1‑300 (MQ=255)                                                                                               cAGCTACTCGGCGCGGAAACTGGGGCTGCAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGAGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCAACATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCAACGTCGGTAACGATGTTGa                                                                                                                                                                                                    >  1:191706/1‑300 (MQ=255)                                                                                                 gCTACTCGGCGCGGAAACTGGGGCTGAAGCACACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAAGTCGAAGCTTCGAGCAGATGCTCAAATAGATGAGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAGATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGaaa                                                                                                                                                                                                  <  2:5530/300‑1 (MQ=255)                                                                                                              gAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAatat                                                                                                                                                                                     <  2:47979/300‑1 (MQ=255)                                                                                                                agcTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:13809/298‑1 (MQ=255)                                                                                                                aaCTGGGGCTTAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGCTATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  2:581983/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGTGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCGCAAAGAGATGGGCACCGGGCTGGTGGTGNCGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGNTTCTGGATAGAGAATGGCGAAATTCAGTGTCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  2:587935/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGTCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:59083/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTTGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:222976/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCGATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:561912/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:172726/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGAGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:42476/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGGGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:188083/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATGGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGTGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  1:267024/300‑1 (MQ=255)                                                                                                                aaCTGGGGCTGAAAAACACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACNGTTGATTATTCCCGTGGTGCAGCGGGNTTCTGGATAGAGAATGTCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATAc                                                                                                                                                                                   <  2:512002/300‑1 (MQ=255)                                                                                                                  ctttggCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTTTCGGATTGCCGGACGAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGTGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAg                                                                                                                                                                                 <  2:164048/296‑1 (MQ=255)                                                                                                                  cTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGTCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAg                                                                                                                                                                                 <  1:584996/300‑1 (MQ=255)                                                                                                                  cTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGTCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGNGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAg                                                                                                                                                                                 <  1:503299/300‑1 (MQ=255)                                                                                                                  cTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGTTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAg                                                                                                                                                                                 <  2:734330/300‑1 (MQ=255)                                                                                                                  cTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTNCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGNTAACGATATTGAAACACGCAGTAATATACAg                                                                                                                                                                                 <  2:532947/300‑1 (MQ=255)                                                                                                                  cTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCACTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAg                                                                                                                                                                                 <  2:122181/300‑1 (MQ=255)                                                                                                                     gggCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAgtgt                                                                                                                                                                              <  1:422548/300‑1 (MQ=255)                                                                                                                          gAAAAGCACCGGATATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAAGCCTAAGCTTCGAGCAGATACTCAAAGAGATGTGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATTTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTc                                                                                                                                                                         <  2:348903/300‑1 (MQ=255)                                                                                                                          gAAAAGCACCGGACATGCGTGCGGTATTCACAACTGGCGGATGGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGANGTGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATANAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTc                                                                                                                                                                         <  2:593403/300‑1 (MQ=255)                                                                                                                          gAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAANTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGtgc                                                                                                                                                                         <  1:543623/300‑3 (MQ=255)                                                                                                                          gAAAAGCACCGGACATGCGGGCGGTATGCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGTTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTc                                                                                                                                                                         <  2:288224/300‑1 (MQ=255)                                                                                                                               gCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGc                                                                                                                                                                    >  2:157495/1‑300 (MQ=255)                                                                                                                               gCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGc                                                                                                                                                                    <  1:599895/300‑1 (MQ=255)                                                                                                                                 aCCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTg                                                                                                                                                                  <  2:428741/300‑1 (MQ=255)                                                                                                                                     gACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCAACATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGc                                                                                                                                                              >  2:420481/1‑300 (MQ=255)                                                                                                                                                 gTATTCGCAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCAACGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCAGGAGATGaaaa                                                                                                                                                  >  2:115683/1‑300 (MQ=255)                                                                                                                                                 gTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGTTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGAAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGgagacggaaa                                                                                                                                                  >  2:116635/1‑294 (MQ=255)                                                                                                                                                 gTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATGTTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGaaaa                                                                                                                                                  >  2:707377/1‑300 (MQ=255)                                                                                                                                                 gTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGaaaa                                                                                                                                                  >  2:712282/1‑300 (MQ=255)                                                                                                                                                 gTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGaaaa                                                                                                                                                  >  1:127147/1‑300 (MQ=255)                                                                                                                                                                  aTTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTaaaaa                                                                                                                                 <  1:615292/300‑1 (MQ=255)                                                                                                                                                                     gCCGGACAAGGTCTAAGCGTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGTCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATCTACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAc                                                                                                                              <  2:467628/300‑1 (MQ=255)                                                                                                                                                                              ggTCTAAGCTTCGAGCAGATGCTCCAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCACGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCAACATCGCAGGTAATTCAAAAGATATGTGGCGCAATATTGCCAACGTAGGTAACGATATTGAAACACGCAGTACTATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCACCGGACAGTAGAAAAACTGGCGCGAc                                                                                                                     >  2:371814/1‑300 (MQ=255)                                                                                                                                                                              ggTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGANTTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGNTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGAc                                                                                                                     >  2:682874/1‑300 (MQ=255)                                                                                                                                                                              ggTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGAc                                                                                                                     >  1:63829/1‑300 (MQ=255)                                                                                                                                                                              ggTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGACGGACAGTAAAAAAACTGGCGCGAc                                                                                                                     >  2:568215/1‑300 (MQ=255)                                                                                                                                                                              ggTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCAACGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGAc                                                                                                                     >  2:75863/1‑300 (MQ=255)                                                                                                                                                                              ggTCTAAGCTTAGAGCAGATGCTCAAAGAGANGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGNGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTAGCGCAATATTGTCACCGTCGGTAAAGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCAGGACAGTAAAAAAACTGGCGCGAc                                                                                                                     >  2:532662/1‑300 (MQ=255)                                                                                                                                                                                               gATGCTCAAAGAGAGGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCTGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACt                                                                                                    <  2:317500/300‑1 (MQ=255)                                                                                                                                                                                                              gggCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACaaa                                                                                     >  2:713817/1‑300 (MQ=255)                                                                                                                                                                                                                     gggCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCAACGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAACCATTc                                                                              >  2:114476/1‑300 (MQ=255)                                                                                                                                                                                                                                   ggAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAACGGCGAAATTCAGGATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAGAATCATTCGCACTGCATCgaga                                                                <  2:330274/300‑1 (MQ=255)                                                                                                                                                                                                                                             ggCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa                                                      >  2:94426/1‑300 (MQ=255)                                                                                                                                                                                                                                              gCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa                                                     >  1:378595/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAggg                                               >  2:433716/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGTAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                         <  1:488958/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       tGATTATTCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAg                            <  1:627344/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             ttccctgtgtgCAGCGGGCTTCTGGATAGAGAATGTCGAAATTCAGTATCCGTTGAGCGAAATCACCATCGCAGGTGATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGTAGATGAAANTCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAACTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGANTGAGGGCCGGGGAGCGTACTCGCAGTACGTg                      <  2:515947/292‑1 (MQ=255)                                                                                                                                                                                                                                                                             ttCCCGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGTTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGTAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTg                      <  2:242374/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               cccGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAc                    <  1:275081/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               cccGTGGGGCAGCGGGCTTCTGGATAGAGAATGTCGACATCTAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATGTAAAAGATATGTTGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGTAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTTCATCGAGACGGAAAGTGAATGAGGGCCGGGGGGCGTACTCGCAGTACGTGAc                    <  2:211884/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cGTGGTGCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAccc                  <  2:457661/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gtgCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGAACTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTc              >  2:392414/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gtgCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCAACATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTc              >  1:380010/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gtgCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCAACATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAACCGGTc              >  1:393763/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      tgCAGCGGGCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCg             <  1:431974/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tgCAGCGGGCTGCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGTTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCg             <  2:409678/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             ggCTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAAATAAAAAATTACAGAAAATCATTCGCACTGCATCGAGAAGGNAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGc      >  2:549548/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cTTCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATTTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGt    <  1:49089/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 tCTGGATAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAg  >  1:213689/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       AAGGGCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGGTATTCACAACTGGCGGATTGCCGGACAAGGTCTAAGCTTCGAGCAGATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGACGGAATTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGGTAGAGAATGGCGAAATTCAGTATCCGGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG  >  NC_000913/4458854‑4459441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑