Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,464	G→A	P480L (CCG→CTG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,464	0	G	A	100.0%	327.4 / NA	93	P480L (CCG→CTG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (60/33);  total (60/33)

GTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAA  >  NC_000913/4283174‑4283754                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                   gTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCNGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACNGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGa                                                                                                                                                                                                                                                                                           >  2:688377/1‑300 (MQ=255) gTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGAACGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGa                                                                                                                                                                                                                                                                                           >  1:631488/1‑300 (MQ=255)        attatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAg                                                                                                                                                                                                                                                                                    <  2:87798/300‑1 (MQ=255)        attatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACNGCGCGCGGAACAGTTCACGCTCACGCNCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTGTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAg                                                                                                                                                                                                                                                                                    >  2:490969/1‑300 (MQ=255)           attggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCNCGCCTTCCGCTGAGTTATCGGTTGCCNAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGtac                                                                                                                                                                                                                                                                                 <  2:500805/297‑1 (MQ=255)           atGGGAAAAGGTGATGCATTTGGTAGAGGAAGAGTATTCCCCGGTCAGACGACCNGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGtac                                                                                                                                                                                                                                                                                 <  2:541166/300‑1 (MQ=255)           atGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGtac                                                                                                                                                                                                                                                                                 >  1:616228/1‑300 (MQ=255)             gggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGtacta                                                                                                                                                                                                                                                                               >  1:490584/1‑300 (MQ=255)                aaaaGGTGATGCATTTGGTAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTg                                                                                                                                                                                                                                                                            <  2:463042/300‑1 (MQ=255)                aaaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGNATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTg                                                                                                                                                                                                                                                                            <  1:506165/300‑1 (MQ=255)                aaaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTg                                                                                                                                                                                                                                                                            <  1:92328/300‑1 (MQ=255)                aaaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTCTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTg                                                                                                                                                                                                                                                                            <  2:465996/300‑1 (MQ=255)                aaaaGGNGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGANGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCTAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTg                                                                                                                                                                                                                                                                            <  2:604873/300‑1 (MQ=255)                   aGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCgg                                                                                                                                                                                                                                                                         <  1:64312/300‑1 (MQ=255)                               tGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCcca                                                                                                                                                                                                                                                             >  1:753236/1‑300 (MQ=255)                                 ggAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCACCGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCAGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCACATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTACTCAGcccccgc                                                                                                                                                                                                                                                           >  2:256298/1‑297 (MQ=255)                                  gagaGGAAGAGTATTCCCCGTTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTTTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCccagcc                                                                                                                                                                                                                                                          <  2:78885/300‑1 (MQ=255)                                       gAAGAGTATTCCCCGGTCAGACGACCGGGGAAGTGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAgccagcca                                                                                                                                                                                                                                                     >  1:662181/1‑300 (MQ=255)                                       gAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCAGAGAAGAACCAGATGCCGAGGAATGCCAAGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTAATGCGGTAATCAGCCCCAgccagcca                                                                                                                                                                                                                                                     >  2:329877/1‑300 (MQ=255)                                        aaGAGTATTCCCCGGTCAGACGACCGGGGAAGGGTAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACANTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCTAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCAc                                                                                                                                                                                                                                                    <  2:650974/300‑1 (MQ=255)                                               ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCcatcat                                                                                                                                                                                                                                             <  1:100541/300‑1 (MQ=255)                                                   ccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATcgcg                                                                                                                                                                                                                                         >  1:493540/1‑300 (MQ=255)                                                            cgacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATNAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTg                                                                                                                                                                                                                                >  2:553537/1‑300 (MQ=255)                                                                 ggggAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGGCAg                                                                                                                                                                                                                           <  1:746330/300‑1 (MQ=255)                                                                   ggAAGGGGAGAAATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCAACCATCATCGCGCCACGCGTGGTCAGtt                                                                                                                                                                                                                         >  2:573126/1‑300 (MQ=255)                                                                    gAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGttt                                                                                                                                                                                                                        <  1:45746/300‑1 (MQ=255)                                                                       ggggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCAGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTACTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTAAGGCCGAGGATCATCAGTACTACTGTGGTAATCAGCCCCAGCCGGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGa                                                                                                                                                                                                                     >  2:301013/1‑300 (MQ=255)                                                                             agaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGNCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCNTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCTCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTa                                                                                                                                                                                                               >  2:595731/1‑300 (MQ=255)                                                                             agaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTa                                                                                                                                                                                                               >  1:319405/1‑300 (MQ=255)                                                                             agaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCAGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTa                                                                                                                                                                                                               >  1:319849/1‑300 (MQ=255)                                                                                   aTGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGa                                                                                                                                                                                                         <  1:668085/300‑1 (MQ=255)                                                                                            gCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCGTACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTGCTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCAACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAAt                                                                                                                                                                                                >  2:637506/1‑300 (MQ=255)                                                                                            gCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGCAAGCAgcat                                                                                                                                                                                                >  2:324018/1‑297 (MQ=255)                                                                                            gCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAAt                                                                                                                                                                                                >  1:41126/1‑300 (MQ=255)                                                                                            gCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCAACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAAt                                                                                                                                                                                                >  2:318521/1‑300 (MQ=255)                                                                                            gCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACCACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGAACAGTACATAGCAAGCAGAAt                                                                                                                                                                                                >  1:444305/1‑300 (MQ=255)                                                                                            gCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCAAGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAAt                                                                                                                                                                                                >  1:346122/1‑300 (MQ=255)                                                                                                      aCGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAAATGGCTTTTTCGTGACCNAGGATCTGTACCCAAACCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCTCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAg                                                                                                                                                                                      >  2:178857/1‑300 (MQ=255)                                                                                                      aCGCCAAAGCAGGTCTGGAAGCGGATAAACTGCGCGCGGAGCAGTTCACACTCCCGCGCGCCTTCCACTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCAAGGAATGCCACGGTGATAGCGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCNAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGATAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGCTTCGACCGGTACATAAAAAGCAGAATGATCGGGAAg                                                                                                                                                                                      >  2:181619/1‑300 (MQ=255)                                                                                                       cGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGt                                                                                                                                                                                     >  1:468146/1‑300 (MQ=255)                                                                                                       cGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGAACAGTACATAGAAAGCAGAATGATCGGGAAGt                                                                                                                                                                                     >  1:322208/1‑300 (MQ=255)                                                                                                              gCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCt                                                                                                                                                                              <  1:411292/300‑1 (MQ=255)                                                                                                                           cGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCa                                                                                                                                                                 >  2:567922/1‑300 (MQ=255)                                                                                                                           cGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCa                                                                                                                                                                 >  1:737872/1‑300 (MQ=255)                                                                                                                           cGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCAACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACGCATCATCGCGCAACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCa                                                                                                                                                                 >  2:634868/1‑300 (MQ=255)                                                                                                                           cGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCAGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGAACAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCa                                                                                                                                                                 >  1:415309/1‑300 (MQ=255)                                                                                                                                     gcgcgcGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCTAGAAGAACCAGATGCCGAGGAATGCCACGTTGAGAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGcc                                                                                                                                                       <  2:207451/300‑1 (MQ=255)                                                                                                                                      cgcgcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCGGCCACCCATCATCGCGCCACGCGTGGGCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGGTACAGCGCGCCGCGATGGCAAACGCCAgtac                                                                                                                                                      <  2:72826/300‑4 (MQ=255)                                                                                                                                       gcgcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGcccc                                                                                                                                                     >  1:149029/1‑300 (MQ=255)                                                                                                                                       gcgcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGcccc                                                                                                                                                     >  2:483187/1‑300 (MQ=255)                                                                                                                                       gcgcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGAACAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCAGCGATGGTACACGCCAGccca                                                                                                                                                     >  2:316659/1‑299 (MQ=255)                                                                                                                                            gAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCAt                                                                                                                                                <  1:453741/300‑1 (MQ=255)                                                                                                                                                 gTTCACGCTCACGCGCGCCTTCCGCTGCGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCAACGATGATAGAGAACAGCGCCGGGTATTCCTACGGGAAGATGGCTTTTTCGTGAACAAGAATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAACCCCAGCAAGCCCCCCATCATCGCGCCACGCGTAGTCGGTTTCGAATAGTACCTAGAAAGCAGAATGATCGGGAAGGGATAGATCGACGCGATGGCAAACGCCAGcgccgctatgaagg                                                                                                                                           >  2:101097/1‑287 (MQ=255)                                                                                                                                                 gTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCCGCCCCAGCCAGCCACCCATCATCGCGCCCCGCGTGGTCAGTTTCGAACAGTAAATAGAAAGAAGAATGATCGGGAAGTTACAGCTCGCAGCGATGGCAAACGCCAGACCCACCATAAAgg                                                                                                                                           >  2:101116/1‑300 (MQ=255)                                                                                                                                                 gTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAgg                                                                                                                                           <  2:468452/300‑1 (MQ=255)                                                                                                                                                 gTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAgg                                                                                                                                           <  1:326916/300‑1 (MQ=255)                                                                                                                                                 gTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGAGAAGTTACAGCTCGCAGCGATGGCCAACGACAGCCCCACCATAAAgg                                                                                                                                           >  2:326052/1‑300 (MQ=255)                                                                                                                                                 gTTCACCCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTNATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCNGCGATGGCAAACGCCAGCCCCACCATAAAgg                                                                                                                                           <  2:532569/300‑1 (MQ=255)                                                                                                                                                                 gcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGNCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATtctc                                                                                                                           >  1:510989/1‑300 (MQ=255)                                                                                                                                                                     tCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAAc                                                                                                                       <  2:300679/300‑1 (MQ=255)                                                                                                                                                                             gTTATCGGTTGCCGAGAAGAACCAAATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAGTGATCGGGAAGTTACAGCTCGCAGCGATGGCAAACGCAAGCCCCACCATAAAGGCGATGTTATGATTATCAAACAGCACGca                                                                                                               >  2:75776/1‑299 (MQ=255)                                                                                                                                                                                        ccGAGAAGAACCAGATGCCTAGGAATGCCACGTTGATAGAGAACAGCGCCGGGTATCCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACGGCGGTAATCAGCCCCAGCCAGCCACCTATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGACTGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAAtgg                                                                                                    <  2:273784/300‑3 (MQ=255)                                                                                                                                                                                                      aTGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAANCAGCCCCAGCCAGCCACCCATCATCGNGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAACCAGCACGCCGAGGATAATCGCAATCACGCCGAgg                                                                                      >  2:508341/1‑300 (MQ=255)                                                                                                                                                                                                              gAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTAc                                                                              <  1:593922/300‑1 (MQ=255)                                                                                                                                                                                                                        gTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACNCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGNTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGAttttt                                                                    >  2:582050/1‑300 (MQ=255)                                                                                                                                                                                                                        gTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGAttttt                                                                    >  1:142704/1‑300 (MQ=255)                                                                                                                                                                                                                        gTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAACCGCAACCACGCAGAGGATCAGTACGGTGAttttt                                                                    >  2:138480/1‑300 (MQ=255)                                                                                                                                                                                                                        gTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTAAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGAttttt                                                                    >  2:331811/1‑300 (MQ=255)                                                                                                                                                                                                                        gTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTCTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATTACGCAGAGGATCAGTACGGTGGttttt                                                                    >  2:421788/1‑300 (MQ=255)                                                                                                                                                                                                                        gTGATAGAGAACAGCGCAGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGGttttt                                                                    >  1:143020/1‑300 (MQ=255)                                                                                                                                                                                                                           aTAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGAt                                                                 >  1:433481/1‑300 (MQ=255)                                                                                                                                                                                                                           aTAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCANCATCGCGCCACGCGTGGTCAGTTTCGNCCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCCGTACGGTGATTTTTGAt                                                                 >  2:485238/1‑300 (MQ=255)                                                                                                                                                                                                                           aTAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCANCATCGCGCCACGCGTGGTCAGTTTCGNCCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGAAATCACGCCGAGGATCAGTACGGTGATTTTTGAt                                                                 >  2:499354/1‑300 (MQ=255)                                                                                                                                                                                                                                gaacagtaccGGGTACTCATACGGAAAGATGGCTTTTTCGTGACCAAGGATCTTTACCAAAATCGTCAGGCCGAGGATCATCAGTACTCCTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCACGCCACGCGTGGTCAGTTTCGAACAGTAAATAGAAAGCAGAATGATCGGGAAGTTAGAGCTCGCCGCGATGGCAAATGCAAGCCAAAACATAAAGGCGATGTTCTGATTCTCAACCAGCACGCCGAGAATAATCGCAATCACGCAGAAGATCAGTACGGTGATTTTTGAtcaccg                                                            >  2:134096/9‑295 (MQ=255)                                                                                                                                                                                                                                gaACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCg                                                            >  1:700052/1‑300 (MQ=255)                                                                                                                                                                                                                                       gcCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCCTCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGTTGATTTTTGATACCCGCAGctct                                                     >  1:657728/1‑300 (MQ=255)                                                                                                                                                                                                                                            gTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCCGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAACGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTcccg                                                >  1:373431/1‑297 (MQ=255)                                                                                                                                                                                                                                            gTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACg                                                >  1:570736/1‑300 (MQ=255)                                                                                                                                                                                                                                              aTTCGTACGGGAGGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCTAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGGGGTCAGTTTCGACCAGTACATAGAAAGNAGAATGATCGGGAAGTTACAGCTCGCNGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGtt                                              <  2:506124/300‑1 (MQ=255)                                                                                                                                                                                                                                              aTTCATACTGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGtt                                              <  1:249994/300‑1 (MQ=255)                                                                                                                                                                                                                                              aTTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGtt                                              <  1:573690/300‑1 (MQ=255)                                                                                                                                                                                                                                                  agaCGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGt                                          <  1:176680/298‑1 (MQ=255)                                                                                                                                                                                                                                                        gAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGcc                                    >  1:365579/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCttt                                 >  1:463082/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           ccAAGGATCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCg                 >  1:443197/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 aTCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTANAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGTTCGCGCCTTTTTTGAAGACGTTAGCGTACAAg           <  2:168785/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aTCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAATGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACACCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGGCGCGCCTTTTTTGAAGACGTTAGCGTACAAg           <  2:729534/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aTCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAg           <  2:361505/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCNGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATNTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGt          <  2:585108/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tCTGTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGt          >  1:243455/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gTACCCAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGCTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGCAGACGTTAGCGTACAAGTCAt       >  2:314073/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          cAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGaa  >  2:299575/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          cAAATCGTCAGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTAACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGaa  >  2:470751/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                   GTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAA  >  NC_000913/4283174‑4283754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑