Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,198,187	G→A	G158S (GGC→AGC)	hemE →	uroporphyrinogen decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,198,187	0	G	A	100.0%	309.4 / NA	87	G158S (GGC→AGC)	hemE	uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/47);  total (40/47)

GCTGCGTCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAGGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCAAAGGCGGCG  >  NC_000913/4197904‑4198472                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                              gCTGCGTCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCAGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGc                                                                                                                                                                                                                                                                               <  1:465479/300‑1 (MQ=255)  cTGCGTCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGcc                                                                                                                                                                                                                                                                              <  1:579591/300‑1 (MQ=255)      gTCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCa                                                                                                                                                                                                                                                                          >  1:53609/1‑300 (MQ=255)       tCGCTACCCGCTGGATGCTGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCAc                                                                                                                                                                                                                                                                         <  2:219480/300‑1 (MQ=255)       tCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGTACGCTATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCAc                                                                                                                                                                                                                                                                         <  2:219240/300‑1 (MQ=255)       tCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCAc                                                                                                                                                                                                                                                                         >  2:115028/1‑300 (MQ=255)        cGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCAcc                                                                                                                                                                                                                                                                        <  1:172087/300‑1 (MQ=255)         gCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCg                                                                                                                                                                                                                                                                       <  1:449036/300‑1 (MQ=255)                gCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCaa                                                                                                                                                                                                                                                                >  2:2182/1‑300 (MQ=255)                                  ctTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGTAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGa                                                                                                                                                                                                                                              <  1:271996/300‑1 (MQ=255)                                    tttCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATc                                                                                                                                                                                                                                            <  1:146973/300‑1 (MQ=255)                                      tCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCg                                                                                                                                                                                                                                          >  1:224639/1‑300 (MQ=255)                                            atCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGgcg                                                                                                                                                                                                                                    >  2:726614/1‑300 (MQ=255)                                            atCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGgcg                                                                                                                                                                                                                                    >  1:456774/1‑300 (MQ=255)                                             tCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGgcgc                                                                                                                                                                                                                                   >  1:379377/1‑300 (MQ=255)                                              ccTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCt                                                                                                                                                                                                                                  >  1:641346/1‑300 (MQ=255)                                               cTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTg                                                                                                                                                                                                                                 <  1:139316/300‑1 (MQ=255)                                                      tGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGctc                                                                                                                                                                                                                          <  2:663841/300‑1 (MQ=255)                                                          ggACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTAGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCAACGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCt                                                                                                                                                                                                                      >  2:311120/1‑300 (MQ=255)                                                           gACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTc                                                                                                                                                                                                                     <  1:447934/300‑1 (MQ=255)                                                                gATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATaa                                                                                                                                                                                                                <  1:566673/300‑1 (MQ=255)                                                                 aTGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATaaa                                                                                                                                                                                                               >  1:711798/1‑300 (MQ=255)                                                                    gggTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTCCCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTg                                                                                                                                                                                                            >  1:165035/1‑300 (MQ=255)                                                                     ggTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTgg                                                                                                                                                                                                           >  1:343100/1‑300 (MQ=255)                                                                     ggTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTAGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCAACGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTgg                                                                                                                                                                                                           >  1:60345/1‑300 (MQ=255)                                                                     ggTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTAACCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATCCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAACAAAATGATGTATACCGATCCACAGGCGCTGCACGCTCTGCTCGATAAACTgg                                                                                                                                                                                                           >  2:309767/1‑300 (MQ=255)                                                                     ggTTAGGGCTCTATTTTGAAGCCGGAGAAGGNCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCNGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTgg                                                                                                                                                                                                           >  2:529576/1‑300 (MQ=255)                                                                            gCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGNCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAg                                                                                                                                                                                                    >  1:531063/1‑300 (MQ=255)                                                                                  ttttGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTAGATAAACTGGCGAAAAGCGTCAc                                                                                                                                                                                              <  1:703844/300‑1 (MQ=255)                                                                                        aGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGNAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTa                                                                                                                                                                                        >  2:647977/1‑300 (MQ=255)                                                                                         gcaggAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTAt                                                                                                                                                                                       >  2:257893/4‑300 (MQ=255)                                                                                              agaAGTTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCGGACGTCGATAAACTGCCAATTCCGGACCCGTAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGaa                                                                                                                                                                                  <  2:269164/300‑1 (MQ=255)                                                                                              agaAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCNTTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCTGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGaa                                                                                                                                                                                  <  1:498731/300‑1 (MQ=255)                                                                                                       gcgTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGAt                                                                                                                                                                         >  2:669516/1‑300 (MQ=255)                                                                                                        cgTTTTACCTCGTCAGTCACCGGCAAAGCTGACGTCGATAAACTGCCAATTCCGNACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGGGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGNACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGAtt                                                                                                                                                                        <  2:689947/300‑1 (MQ=255)                                                                                                        cgTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGNACCCGGAAGATGAACTGGGTTACTTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGNACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGAtt                                                                                                                                                                        <  2:689934/300‑1 (MQ=255)                                                                                                             tACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACTTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGc                                                                                                                                                                   <  1:490138/300‑1 (MQ=255)                                                                                                             tACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGNAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGc                                                                                                                                                                   <  1:506592/300‑1 (MQ=255)                                                                                                             tACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGc                                                                                                                                                                   <  1:490146/300‑1 (MQ=255)                                                                                                             tACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGc                                                                                                                                                                   <  2:577660/300‑1 (MQ=255)                                                                                                                        tCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAgg                                                                                                                                                        <  1:257949/300‑1 (MQ=255)                                                                                                                         cACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGc                                                                                                                                                       <  1:891/300‑1 (MQ=255)                                                                                                                         cACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCAGGTGCTCAGGc                                                                                                                                                       >  2:379530/1‑300 (MQ=255)                                                                                                                           ccTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAg                                                                                                                                                     <  1:302919/300‑1 (MQ=255)                                                                                                                                   gCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATTATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGAtt                                                                                                                                             <  2:242793/300‑1 (MQ=255)                                                                                                                                        cGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGa                                                                                                                                        >  1:606016/1‑300 (MQ=255)                                                                                                                                            aaTAAACTGCCAATTCCGGACCCGGAAGATGAACGGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGGTTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACAcc                                                                                                                                    <  1:219112/299‑1 (MQ=255)                                                                                                                                                  cTGCCAATTCCGTACCCGGAAGATGAACTGGTTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGggc                                                                                                                              <  1:305293/300‑1 (MQ=255)                                                                                                                                                  cTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGggc                                                                                                                              <  1:73247/300‑1 (MQ=255)                                                                                                                                                              gACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACTTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTAcc                                                                                                                  <  1:78443/300‑1 (MQ=255)                                                                                                                                                              gACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTAcc                                                                                                                  <  1:618051/300‑1 (MQ=255)                                                                                                                                                               aCCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCNACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCg                                                                                                                 <  1:525318/300‑1 (MQ=255)                                                                                                                                                               aCCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCg                                                                                                                 <  1:372995/300‑1 (MQ=255)                                                                                                                                                                          gAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTAt                                                                                                      >  2:332914/1‑300 (MQ=255)                                                                                                                                                                              tGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAAc                                                                                                  <  2:701001/300‑1 (MQ=255)                                                                                                                                                                               gggTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGTAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACa                                                                                                 >  2:208827/1‑300 (MQ=255)                                                                                                                                                                               gggTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACa                                                                                                 >  1:169191/1‑300 (MQ=255)                                                                                                                                                                               gggTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCAATTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACa                                                                                                 >  2:405108/1‑300 (MQ=255)                                                                                                                                                                                 gTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCAGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCAATTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGt                                                                                               >  1:267889/1‑300 (MQ=255)                                                                                                                                                                                   tACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCAGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGAGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTtc                                                                                             >  2:33744/1‑300 (MQ=255)                                                                                                                                                                                        gatgaACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGct                                                                                        >  2:17655/1‑300 (MQ=255)                                                                                                                                                                                               gcgGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTAc                                                                                 <  1:359341/300‑1 (MQ=255)                                                                                                                                                                                                cgGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACa                                                                                >  1:587583/1‑300 (MQ=255)                                                                                                                                                                                                 ggTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACAt                                                                               <  1:551490/300‑1 (MQ=255)                                                                                                                                                                                                 ggTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCGGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAANGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGCCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACac                                                                               <  2:648301/300‑2 (MQ=255)                                                                                                                                                                                                  gTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGCTGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATg                                                                              >  1:662336/1‑300 (MQ=255)                                                                                                                                                                                                  gTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCCACAGTTCTCGCTCTATTACATg                                                                              >  1:377038/1‑300 (MQ=255)                                                                                                                                                                                                  gTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACAta                                                                              >  1:377091/1‑299 (MQ=255)                                                                                                                                                                                                  gTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATg                                                                              <  2:221754/300‑1 (MQ=255)                                                                                                                                                                                                  gTGCGTACCATTCATCGCAAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGCTGTATGCCGATTCGCAGGCGCTGCACGCTCTGCTCCATCAACTGGCTAAACGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGTTGCTCAGGCAGTGGTGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATg                                                                              >  2:372433/1‑300 (MQ=255)                                                                                                                                                                                                   tGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGc                                                                             <  1:306390/300‑1 (MQ=255)                                                                                                                                                                                                    gCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCa                                                                            >  1:673183/1‑300 (MQ=255)                                                                                                                                                                                                    gCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCAATTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCa                                                                            >  2:396847/1‑300 (MQ=255)                                                                                                                                                                                                      gTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCNCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGNGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTAAATGCATa                                                                          >  2:681950/1‑300 (MQ=255)                                                                                                                                                                                                          cATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAAt                                                                      <  1:585884/300‑1 (MQ=255)                                                                                                                                                                                                               gtcgCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAANAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAAttgttg                                                                 <  1:498638/300‑1 (MQ=255)                                                                                                                                                                                                                        tGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTAc                                                        <  2:39159/300‑1 (MQ=255)                                                                                                                                                                                                                          aaaGGCGAAGTGCCGCTGATTTGTTTGTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCATTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTg                                                      <  2:213773/300‑1 (MQ=255)                                                                                                                                                                                                                                gAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGaa                                                <  2:627383/300‑1 (MQ=255)                                                                                                                                                                                                                                                      ccGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAATCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCGGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCgg                          <  1:684844/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gCCCGTGGACGCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGc                    >  1:663350/1‑300 (MQ=255)                                                                                                                                                                                                                                                             ctcgttgacgccggctacctatgggttggAAAGCGCCAGCAGCAAAGACGCCACCGTAATCAAAAAAAGGATGCATCCCGATCCACAGGCGCTGCACGCTCTACTCGATAAACTGGCGAAAAGCTTCACTTTGCATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTTGGGCGGCGGGCTTACCGGGCGCGATTATCAACAGGTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGTTCACGCt                   <  2:112313/274‑1 (MQ=255)                                                                                                                                                                                                                                                                      gccggCGACCTACATGGTGGAAAACTGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACCGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGAGTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTGACCGGGCGCGATTATCAACAGTTCTCACTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCaa          <  2:37391/297‑1 (MQ=255)                                                                                                                                                                                                                                                                      gCTGGCGACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCaa          <  2:38539/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gACCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCAAAggcgg    >  2:35839/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             aCCTACATGGTGGAAAGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCAAAggcggc   >  1:52077/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ccTACATGGTGGAAAGCGGCAGCAGCAAAGCNTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAANGCGCAGATTAAAGCCGGTGCTCAGGCGGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCAAAAgcggcg  >  2:516579/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                              GCTGCGTCGCTACCCGCTGGATGCGGCGATCCTCTTTTCCGATATCCTCACCGTGCCGGACGCGATGGGGTTAGGGCTCTATTTTGAAGCCGGAGAAGGTCCGCGTTTTACCTCGCCAGTCACCTGCAAAGCTGACGTCGATAAACTGCCAATTCCGGACCCGGAAGATGAACTGGGTTACGTGATGAACGCGGTGCGTACCATTCGTCGCGAACTGAAAGGCGAAGTGCCGCTGATTGGTTTTTCCGGCAGCCCGTGGACGCTGGCGACCTACATGGTGGAAGGCGGCAGCAGCAAAGCCTTCACCGTGATCAAAAAAATGATGTATGCCGATCCGCAGGCGCTGCACGCTCTGCTCGATAAACTGGCGAAAAGCGTCACTTTGTATCTGAATGCGCAGATTAAAGCCGGTGCTCAGGCAGTGATGATTTTCGACACCTGGGGCGGCGTGCTTACCGGGCGCGATTATCAACAGTTCTCGCTCTATTACATGCATAAAATTGTTGATGGTTTACTGCGTGAAAACGACGGTCGCCGCGTACCGGTCACGCTGTTTACCAAAGGCGGCG  >  NC_000913/4197904‑4198472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑