Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,017,742	G→A	S137N (AGT→AAT)	rmuC →	putative recombination limiting protein RmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,742	0	G	A	100.0%	334.8 / NA	95	S137N (AGT→AAT)	rmuC	putative recombination limiting protein RmuC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (58/37);  total (58/37)

GAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAGTCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAAT  >  NC_000913/4017450‑4018031                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                  tagagggTCGCGGAGTTAAGCGCGGCAAAACAACAAAGTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGTCTGCAGAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTAAAc                                                                                                                                                                                                                                                                                            <  2:278363/295‑1 (MQ=255) gagaTGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAAc                                                                                                                                                                                                                                                                                            <  1:150441/300‑1 (MQ=255) gagaTGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCCCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAAGTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAAc                                                                                                                                                                                                                                                                                            <  1:48935/300‑1 (MQ=255)        tCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTCACACCTCTCTGGAGGCCGATTTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCAAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCACCCGTATTTTTGAGTACAGTAATCGCCGGGTTGATGAGCAAAATCGTCAGAATCTGAACAGCCtgt                                                                                                                                                                                                                                                                                     >  2:137606/1‑300 (MQ=255)           cgGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCtgttgt                                                                                                                                                                                                                                                                                  <  1:741042/300‑1 (MQ=255)             gAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTcg                                                                                                                                                                                                                                                                                >  1:375836/1‑300 (MQ=255)             gAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCAGGGTTGATGAGCAAAAACGTAAGAATCTGAACAGCCTGTTGTcg                                                                                                                                                                                                                                                                                >  2:358039/1‑300 (MQ=255)                          cAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGaac                                                                                                                                                                                                                                                                   <  1:457419/300‑1 (MQ=255)                             aacaacAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGaacaac                                                                                                                                                                                                                                                                <  1:15419/300‑1 (MQ=255)                                   aaaTTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACg                                                                                                                                                                                                                                                          >  1:167360/1‑300 (MQ=255)                                                  aGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAgg                                                                                                                                                                                                                                           <  2:634147/300‑1 (MQ=255)                                                   gCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGt                                                                                                                                                                                                                                          >  1:148959/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAgg                                                                                                                                                                                                                                     >  2:294469/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAgg                                                                                                                                                                                                                                     >  1:32275/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGNATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAgg                                                                                                                                                                                                                                     >  1:534289/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTAAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTACGCCGTCAGGTTCAgg                                                                                                                                                                                                                                     >  2:295381/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTAAGTGAGCAGTTTGAAAACCTCGCAAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAgg                                                                                                                                                                                                                                     >  1:169438/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTAAGTGAGCAGTTTGAAAACCTCGACAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAgg                                                                                                                                                                                                                                     >  2:725263/1‑300 (MQ=255)                                                        ggCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTATGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGCCGCTAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCCCCGCAATCGCCGGGTTGCTGAGCAAACCCGTCAGCATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAgt                                                                                                                                                                                                                                     >  2:115666/1‑299 (MQ=255)                                                          cGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACTGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGAc                                                                                                                                                                                                                                   <  2:318376/300‑1 (MQ=255)                                                          cGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGAc                                                                                                                                                                                                                                   <  1:131301/300‑1 (MQ=255)                                                                gggTGCGAGTTACTCAATAACGAAGTGCGCAGCATGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCCAAACCGTAAGAATCTGAACAGCCTGTTNTCGCCGCTACGTGAACAACTGGACGGTCTCCGCCGTCAGGTCCCGGACAGCtta                                                                                                                                                                                                                             >  2:550625/3‑299 (MQ=255)                                                                gagTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTc                                                                                                                                                                                                                             >  2:131671/1‑300 (MQ=255)                                                                  gtgtgAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACCGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCAGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTNGCCGCTACGTGAACAACTGGACGGTTTCCGCAGTCAGGTTCAGGACANCTTCgg                                                                                                                                                                                                                           >  2:607359/5‑300 (MQ=255)                                                                  gTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTATGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTAAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCttagg                                                                                                                                                                                                                           >  1:250897/1‑297 (MQ=255)                                                                           aCTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCACCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGc                                                                                                                                                                                                                  >  2:442554/1‑300 (MQ=255)                                                                                  aaCGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGaa                                                                                                                                                                                                           >  1:193072/1‑300 (MQ=255)                                                                                     gAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGc                                                                                                                                                                                                        <  1:324648/300‑1 (MQ=255)                                                                                     gAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTAAGTGAGCAGTTTGAAAACCCCGCAAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCATGTTGTCGCCGCTACGTGAACAACTGGATGGTTTCCGCCGTCAGGTTCAGGACAGCTTAGGTAAAGAAGCACAAGAACGc                                                                                                                                                                                                        >  2:9087/1‑300 (MQ=255)                                                                                        gTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGCTCTGCTTGAAGTANCCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCAt                                                                                                                                                                                                     <  2:540951/300‑1 (MQ=255)                                                                                           cgcAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATAcc                                                                                                                                                                                                  <  1:17798/300‑1 (MQ=255)                                                                                            gcAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAANAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATAccc                                                                                                                                                                                                 <  1:498517/300‑1 (MQ=255)                                                                                            gcAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATAccc                                                                                                                                                                                                 <  2:280360/300‑1 (MQ=255)                                                                                            gcAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATAccc                                                                                                                                                                                                 <  1:250283/300‑1 (MQ=255)                                                                                            gcAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTGGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATAccc                                                                                                                                                                                                 <  2:404663/300‑1 (MQ=255)                                                                                               gCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGATCAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGa                                                                                                                                                                                              >  1:455854/1‑300 (MQ=255)                                                                                                 cTGCAACGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCCTATTTTTGAGCACAGCAATCGCCGGGNTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGNTAAAGAAGCACAAGAACGCCATACCCTGAcc                                                                                                                                                                                            <  2:518766/300‑1 (MQ=255)                                                                                                 cTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGAcc                                                                                                                                                                                            >  2:278007/1‑300 (MQ=255)                                                                                                 cTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGAcc                                                                                                                                                                                            <  1:395659/300‑1 (MQ=255)                                                                                                   gCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCa                                                                                                                                                                                          >  1:639743/1‑300 (MQ=255)                                                                                                        gTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCCCAAGAACGCCATACCCTGACCCACGaaa                                                                                                                                                                                     >  1:226802/1‑300 (MQ=255)                                                                                                        gTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGaaa                                                                                                                                                                                     >  1:705991/1‑300 (MQ=255)                                                                                                        gTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGACGGAAGCCGCACCGCAACATGCTGACGATAAAATTCGACAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGCGCACAGCACTCGCCGGGTTGATGAGCACAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGCCGGTTTCCGCCGTCAGGTTCAGGACCGCTTCGGTAAAGAAGCACAAGAACACCATACCCTGACCCACGaaa                                                                                                                                                                                     >  1:741278/1‑300 (MQ=255)                                                                                                          aTTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGACAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTAGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAAtt                                                                                                                                                                                   >  2:294758/1‑300 (MQ=255)                                                                                                              acacCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAGTTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGCTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCa                                                                                                                                                                               >  2:47273/1‑300 (MQ=255)                                                                                                                 ccTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAAtc                                                                                                                                                                            >  1:557976/1‑300 (MQ=255)                                                                                                                   tctctGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCAGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGCACGCCATACCCTGACCCACGAAATTCGCAAtctc                                                                                                                                                                          >  2:316368/1‑300 (MQ=255)                                                                                                                    ctctGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTcc                                                                                                                                                                         >  1:612090/1‑300 (MQ=255)                                                                                                                    ctctGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTACGCCGTCAGGTTCAGGACAGCTTCGGTAACGAAGCACAAGAACGCCATACCCTGACCAACGAAATTCGCAAtctaa                                                                                                                                                                         >  2:248311/1‑298 (MQ=255)                                                                                                                          aGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGAACCACGAAATTCGCAATCTCCAGCAAc                                                                                                                                                                   >  1:20081/1‑300 (MQ=255)                                                                                                                            gCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTc                                                                                                                                                                 >  1:597187/1‑300 (MQ=255)                                                                                                                            gCCGATCTGCGTGAAGTAACCACGCAGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTc                                                                                                                                                                 <  2:287890/300‑1 (MQ=255)                                                                                                                                  cTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAAcgcg                                                                                                                                                           >  1:24434/1‑300 (MQ=255)                                                                                                                                    gCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGACGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACCAGAACGCCATACCCTGAACCACGAAATTCGCAATCTCCAGCAACTCCACGCGCa                                                                                                                                                         >  2:569497/1‑300 (MQ=255)                                                                                                                                         aaGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGGTGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATgg                                                                                                                                                    >  2:579540/1‑300 (MQ=255)                                                                                                                                         aaGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGTAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCNTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCACGACAGCTTCGGTAAAGAAGCACAAGAACGCAATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATgg                                                                                                                                                    >  2:167043/1‑300 (MQ=255)                                                                                                                                             aaCCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTAGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTAAACGCGCAAATGGCCCa                                                                                                                                                >  1:370120/1‑300 (MQ=255)                                                                                                                                                      gATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTNATGAGCAAAACCGTCAGAATCTGAACNGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTAGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACACGCAAATGGCCCAGGAAGCGAt                                                                                                                                       >  2:485288/1‑300 (MQ=255)                                                                                                                                                           aaGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAAcc                                                                                                                                  >  1:76171/1‑300 (MQ=255)                                                                                                                                                                   caGCAACATGCTGACGATAAAATTCGAAAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGAAAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATCCCCTGACCCACGAACTTCGCAATCTCAAGCAAATCAAAGCGCAAATGGCACAGGAAGTGATTAACCTGAcgcgc                                                                                                                          >  2:200036/1‑300 (MQ=255)                                                                                                                                                                     gCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGNACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGAagcgcgc                                                                                                                        >  1:512967/1‑300 (MQ=255)                                                                                                                                                                               gACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGc                                                                                                              <  2:479439/300‑1 (MQ=255)                                                                                                                                                                               gACGATAAAATTCGCCAGATGATGAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGc                                                                                                              <  2:150633/300‑1 (MQ=255)                                                                                                                                                                                            gCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAAccc                                                                                                 <  1:426092/300‑1 (MQ=255)                                                                                                                                                                                                 atgatTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCATGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCCGGACAGCTTCGGTAAAGAAGCACACGCACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCACCGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGCAAGGCGACACTAAAACCAAGGGc                                                                                            >  2:434114/1‑300 (MQ=255)                                                                                                                                                                                                   gatTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCCCAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCAAGGGCaa                                                                                          >  2:203036/1‑300 (MQ=255)                                                                                                                                                                                                   gatTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCaa                                                                                          >  1:58272/1‑300 (MQ=255)                                                                                                                                                                                                        aCAGCGAGCAGCGCCTAAGTGAGCAGTTTGAAAGCCTCGCAAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCGGCATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTACAGAAGCACAAGAACGCCATACCTTGACCCACGAAATTCGCAATCTCCAGCAACTCAACACGCCAATGGCCCAGGAAGCGACTAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCCGGGCAACtcgg                                                                                     >  2:101075/1‑297 (MQ=255)                                                                                                                                                                                                               gcagcGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGgt                                                                              <  1:227031/300‑1 (MQ=255)                                                                                                                                                                                                                  gcgcCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGgtagt                                                                           >  1:559178/1‑300 (MQ=255)                                                                                                                                                                                                                  gcgcCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCACAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCCGGGCAACTGGGGCGAGgtagt                                                                           >  2:478329/1‑300 (MQ=255)                                                                                                                                                                                                                    gccgcagggAGCAGTGTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTAt                                                                         <  2:464478/292‑1 (MQ=255)                                                                                                                                                                                                                    gcCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCTGGTGGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTAt                                                                         <  2:669190/300‑1 (MQ=255)                                                                                                                                                                                                                    gcCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTAt                                                                         >  1:367525/1‑300 (MQ=255)                                                                                                                                                                                                                       tCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTNCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGNAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGGTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGa                                                                      >  2:594695/1‑300 (MQ=255)                                                                                                                                                                                                                       tCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGa                                                                      >  1:277362/1‑300 (MQ=255)                                                                                                                                                                                                                        cAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGAc                                                                     >  1:302089/1‑300 (MQ=255)                                                                                                                                                                                                                         aGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGAcg                                                                    <  1:461341/300‑1 (MQ=255)                                                                                                                                                                                                                         aGTGAGCAGTTCGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCNCCGTCAGGTTCAGGACAGCTTCGGTANAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTAAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGAcg                                                                    >  2:503020/1‑300 (MQ=255)                                                                                                                                                                                                                                             cGCCAANCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGGCANAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCgg                                                <  2:613315/300‑1 (MQ=255)                                                                                                                                                                                                                                               ccAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTTTCGCCGCTACGGGAACAACGGGACGGTTTCTGCCGTCAGGTTCAGGACAGCTTCGGTGAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTc                                              <  2:84091/300‑1 (MQ=255)                                                                                                                                                                                                                                               ccAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGTAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTc                                              <  2:295533/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ccGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCg                                          >  2:729296/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTg                                        >  1:282278/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ttttGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAgg                                    <  1:34313/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAANAACGCCATACCCTGACCCACGAAATTNGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAAATGGGGCGAGGCAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGtatga                              >  2:489905/1‑300 (MQ=255)                                                                                                                                                                                                                                                                cacaGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGtatgaa                             <  1:89048/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGTTAAATAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAAc                     <  2:568711/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCGCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCGCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAAc                     <  2:253333/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          gCCGGGTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAAccc                   <  1:351900/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gggTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAGCCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAgg                <  1:325722/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gggTTGATGAGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAgg                >  1:706017/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gaGCAAAACCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATc        >  1:179107/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         aaaaCCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGaaa    <  1:607225/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aaCCGTCAGAATCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAAt  <  2:56923/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                  GAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTTACTCAATAACGAAGTGCGCAGCCTGCAAAGTATTAACACCTCTCTGGAGGCCGATCTGCGTGAAGTAACCACGCGGATGGAAGCCGCACAGCAACATGCTGACGATAAAATTCGCCAGATGATTAACAGCGAGCAGCGCCTCAGTGAGCAGTTTGAAAACCTCGCCAACCGTATTTTTGAGCACAGCAATCGCCGGGTTGATGAGCAAAACCGTCAGAGTCTGAACAGCCTGTTGTCGCCGCTACGTGAACAACTGGACGGTTTCCGCCGTCAGGTTCAGGACAGCTTCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAAT  >  NC_000913/4017450‑4018031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑