Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,355	T→G	E84D (GAA→GAC)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,355	0	T	G	100.0%	299.0 / NA	92	E84D (GAA→GAC)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/52);  total (40/52)

TGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGTTGATTACGTATTG  >  NC_000913/2003060‑2003646                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                    tGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCgg                                                                                                                                                                                                                                                                                                 >  2:708144/1‑300 (MQ=255)  ggTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTCCCGTCAGTTCCCGCACACGCTGTAAGTTGTTGTTGATGTCGGa                                                                                                                                                                                                                                                                                                >  2:368969/1‑300 (MQ=255)   gTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGAc                                                                                                                                                                                                                                                                                               <  1:573504/300‑1 (MQ=255)                tATTTTTAACGCTAAAACCATCAAGGCCAAGATTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAATACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCgg                                                                                                                                                                                                                                                                                  <  2:414708/300‑1 (MQ=255)                 aTTTTTAACGCTAAAACCATCAAGTCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCggt                                                                                                                                                                                                                                                                                 <  1:723688/300‑1 (MQ=255)                 aTTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGTTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCggt                                                                                                                                                                                                                                                                                 <  1:487891/300‑1 (MQ=255)                  tttttAGCGCTAAAACCATCAAGGCCAAGGTTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCGCGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGTCTTCggtg                                                                                                                                                                                                                                                                                <  1:143057/300‑1 (MQ=255)                     ttAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTc                                                                                                                                                                                                                                                                             <  1:118006/300‑1 (MQ=255)                         cGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTGCCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGGTGTTGTTGATGTCGGACAGCGCGACTTCGGTGGTCTgcg                                                                                                                                                                                                                                                                         >  2:635861/1‑300 (MQ=255)                                aCCATCAAGGTCAAGAGTTTTAGTAGCAATCTGCTTCAGGGCGATGGTGATGGTCTGGTTGTCAGTTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACgg                                                                                                                                                                                                                                                                   <  1:155329/300‑1 (MQ=255)                                       gggCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCATACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACcg                                                                                                                                                                                                                                                           <  2:350729/299‑1 (MQ=255)                                       aGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACcg                                                                                                                                                                                                                                                           <  2:678739/300‑1 (MQ=255)                                       aGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACcg                                                                                                                                                                                                                                                           <  1:366263/300‑1 (MQ=255)                                          aaaggagTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCTAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTGGAACTTGGTCTGACCAGATACGCGGTCAATTTCATCCGGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTGCGGTGGTCTGCGCAACGGAGATACcgtcg                                                                                                                                                                                                                                                        <  2:288361/296‑1 (MQ=255)                                                gTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACAGAGATACCGTCGTTGGCg                                                                                                                                                                                                                                                  >  1:96150/1‑300 (MQ=255)                                                      gCATCAATCTGCTTCAGATCGATATTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACgg                                                                                                                                                                                                                                            <  1:95945/300‑1 (MQ=255)                                                      gCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACgg                                                                                                                                                                                                                                            <  1:252498/300‑1 (MQ=255)                                                        aTCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGgc                                                                                                                                                                                                                                          <  1:744203/300‑1 (MQ=255)                                                         tCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGCTCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACAATCTGTAAGTTGTTTTTGATGTCGGANAGCACGCCATCTGTGGTCTTCGCAACGGAGATACCATCCTTGGGGTTACGGgcc                                                                                                                                                                                                                                         >  2:550544/1‑300 (MQ=255)                                                                  ttCAGATCGATATTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTc                                                                                                                                                                                                                                <  1:262682/300‑1 (MQ=255)                                                                  ttCAGATCGATATTGATAGTCTGGTTATCA‑TCTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTTACGCTGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTc                                                                                                                                                                                                                                <  2:366872/300‑1 (MQ=255)                                                                       aTCGATGGTGAGAGTCTGGTTATCA‑TTTGCGCCAACCTTGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGTTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTGAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGcc                                                                                                                                                                                                                           <  2:11689/300‑1 (MQ=255)                                                                                     tCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAgg                                                                                                                                                                                                             <  2:713838/300‑1 (MQ=255)                                                                                             tCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACgg                                                                                                                                                                                                     <  1:19981/300‑1 (MQ=255)                                                                                              tcatcggcgcCAACCTGGATTGTCATGGAGCCATTTTTTGCCAGCACGTTCACACCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATGTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGTTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACgg                                                                                                                                                                                                     <  2:136867/294‑1 (MQ=255)                                                                                                        cAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCg                                                                                                                                                                                           <  1:683787/300‑1 (MQ=255)                                                                                                          aCCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGcc                                                                                                                                                                                         <  1:377060/300‑1 (MQ=255)                                                                                                          aCCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAAGTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGcc                                                                                                                                                                                         <  2:53427/300‑1 (MQ=255)                                                                                                               gATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGAcc                                                                                                                                                                                    <  1:380697/300‑1 (MQ=255)                                                                                                                    tCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTg                                                                                                                                                                               <  1:434350/300‑1 (MQ=255)                                                                                                                            ccATTTTTTGGCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATcc                                                                                                                                                                       <  1:331591/300‑1 (MQ=255)                                                                                                                            ccATTTTTTGCCAGCACGTTCGCGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATcc                                                                                                                                                                       <  2:342293/300‑1 (MQ=255)                                                                                                                              aTTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCtt                                                                                                                                                                     <  1:55391/300‑1 (MQ=255)                                                                                                                                 ttttGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATNTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACGCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTcgc                                                                                                                                                                  <  2:554680/300‑1 (MQ=255)                                                                                                                                     gCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTg                                                                                                                                                              <  1:395206/300‑1 (MQ=255)                                                                                                                                            cGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTNAGTTCCCGCACACGCTGTAAGTTGTTNTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCACCCTTCGCGCCGTTAATAc                                                                                                                                                       >  2:505530/1‑300 (MQ=255)                                                                                                                                            cGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCTCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATAc                                                                                                                                                       >  2:429708/1‑300 (MQ=255)                                                                                                                                             gTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACg                                                                                                                                                      <  1:146597/300‑1 (MQ=255)                                                                                                                                              ttCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGc                                                                                                                                                     <  1:441088/300‑1 (MQ=255)                                                                                                                                              ttCACGCCGTTGAACTGGGTCTGACCAGATACGCAGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGTGCCGCCTGAGTCAGGCCTTTAATGTTAGAGTTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGc                                                                                                                                                     <  1:482034/300‑1 (MQ=255)                                                                                                                                                    ccGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGCTAGAAGACAGACCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTCCCGTCCGTTCCCGCACACGCTGTCAGTTGTTGTTGACGTCGGACAGCGCGCCTTCNGTGGTCTGCGCAAAGGAGATACCGTCGTTGGGGTTACGGGCCGCCTGAGTTAGGCCTTTCATGCTCGAGGTGAAACGTTTTGCAATTGCCTGACCCGCTGCATCATCCTTCACGCTGTTAATCCGCAAGCCa                                                                                                                                               >  2:181160/1‑300 (MQ=255)                                                                                                                                                      gTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTCGCAATCGCCTGACCCGCTGTGTCATCCTTCGCGCTGTTAATACGCAAGCCaga                                                                                                                                             >  1:646008/1‑300 (MQ=255)                                                                                                                                                        tGAACGGGTTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACACACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCagaag                                                                                                                                           <  1:167129/300‑1 (MQ=255)                                                                                                                                                        ggAACTGGGTCTGACCAGATACGCGTTCAATTTCATCCATACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCagaag                                                                                                                                           <  2:98337/299‑1 (MQ=255)                                                                                                                                                         gAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCagaaga                                                                                                                                          >  1:696568/1‑300 (MQ=255)                                                                                                                                                         gAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGCTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCTGTGGTCTGCGCCACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTTAGTCAGGCCTTTCATGTTCGAGATGAAACGGTTAGCCATCGCATGACCCGCTGCGTCATCCTTCGCGCTGTTCATACGCAAGCCagaaga                                                                                                                                          >  2:202791/1‑300 (MQ=255)                                                                                                                                                             tGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACTGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAagacaga                                                                                                                                      <  2:375859/300‑1 (MQ=255)                                                                                                                                                               ggTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTCCCGTCAGTTCACGCACACGTTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTCATGTTCGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAAAAGACAGACg                                                                                                                                    >  1:285265/1‑300 (MQ=255)                                                                                                                                                               ggTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTCCGGGCCGCCCGAGTCAGGCCCTTAATGTTAGAAGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAAAAGACAGACg                                                                                                                                    >  1:56906/1‑300 (MQ=255)                                                                                                                                                                    gACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTCCCGTCAGTTCACACACACGCCGTAAGTTGTTGTTGACGTCGGACAGCGCGCCTTCGGTGGCCTGCGCAACGGAGATACCGTCGTTGGCGTTCAAGGCCACCTGAGTCAAGCCTTTAATGTTCGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGAGCTGTTAACCCGCAAGTCAGAagaaagacaatcga                                                                                                                               >  2:214584/1‑294 (MQ=255)                                                                                                                                                                    gACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCCCGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTCCGGGACGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCACTGTGTCATCCTTCTCGCTGTTAACCCGCCAGCCAGAAGACAGACGCTCGa                                                                                                                               >  2:199597/1‑300 (MQ=255)                                                                                                                                                                    gACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCNCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCACCCTTCGCGCTGTTAATACGCAAGCCAGAAGAAAGACGATCGa                                                                                                                               >  2:647414/1‑300 (MQ=255)                                                                                                                                                                     aCCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGAt                                                                                                                              <  1:669323/300‑1 (MQ=255)                                                                                                                                                                      ccAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACCCGCTGTAAGTTGTTGTTGGTGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTTGTGTTGCAGGCCGACTGAGTCAGGCCTTTAATGTTAGAGGTGAAACAGTTAGTAATCGCATGAACCACTGCGTCGTCCCTCGCGCTGTTCATAATCAAGCCagaagaaaaacgatcgaga                                                                                                                             >  2:47452/1‑287 (MQ=255)                                                                                                                                                                       caggtACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTTGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGTAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAg                                                                                                                            <  2:398359/296‑1 (MQ=255)                                                                                                                                                                               cgGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTCGAGGTGAAACGGTTCGCAATCGCCTGACCCGCTGCGTCATCCTTCGAGTTGTTAATCCGCAAGCCAGAAGACAGACGCTCTATAGAACTCGAc                                                                                                                    >  1:375611/1‑300 (MQ=255)                                                                                                                                                                                 gTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAg                                                                                                                  <  2:313644/300‑1 (MQ=255)                                                                                                                                                                                  tCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATATAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAgc                                                                                                                 <  2:397176/300‑1 (MQ=255)                                                                                                                                                                                                   ggATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTTGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTCATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCCTCCTTCGCGCTGTTAATACGAAAGCCAGAAGACAGACGCTCGATAGAAATCGACAGCGCAGAATGGTTCttgtt                                                                                                >  2:627612/1‑300 (MQ=255)                                                                                                                                                                                                             tCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTAttt                                                                                      <  1:438325/300‑1 (MQ=255)                                                                                                                                                                                                                    ggATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTCGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGACAGAAGACAGACGCTCGATAGAACTCGAAAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGa                                                                               >  1:570291/1‑300 (MQ=255)                                                                                                                                                                                                                    ggATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCATCCGTCCACGCCCACGCTGTAAGTTGTTGTTGACGTCAGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGACACCGTCGTTGGCGTTACGGGTCGCCTGAGTCAGGCCTTTAATGTTAAAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAACCCGCAAGCCAGAAGAAAAACGCTCGGTAGGACTCGACAGCGCAGGCTGGTTTTTGTTGATATTATTTTGAGTgg                                                                               >  2:365653/1‑299 (MQ=255)                                                                                                                                                                                                                      aTAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATc                                                                             >  1:453979/1‑300 (MQ=255)                                                                                                                                                                                                                           agacagacCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTTTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGATGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCTTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCga                                                                        <  2:254818/300‑1 (MQ=255)                                                                                                                                                                                                                            gaCAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCgag                                                                       <  1:233867/300‑1 (MQ=255)                                                                                                                                                                                                                                gATCAGACTCAGAGTTAGTGCCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGTAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGTTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGc                                                                   <  2:43569/300‑1 (MQ=255)                                                                                                                                                                                                                                gATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTCGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTTTTGTTGATATTATTTTGAGTGATCCGCGAGAGGc                                                                   >  2:215122/1‑300 (MQ=255)                                                                                                                                                                                                                                        tCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTa                                                           <  1:682817/300‑1 (MQ=255)                                                                                                                                                                                                                                           gagTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCCCGCACCCGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACAGAGATACCGTCGTTGGCGTTACGGGCCACCTGAGTCAGGCCTTTAACGTTCGAGGTGAAACGGTTAGCAACNACCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCAACAGCGTAGACGGGTTTTTGTTGGGATTCTTTTGAGTGGTTAGCGAGAGGCTGTTGGTCTTa                                                        >  2:177507/1‑300 (MQ=255)                                                                                                                                                                                                                                              ttAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATg                                                     <  1:153459/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gTACCGGTAGTGGCCTGTACCGTCAGTTCCCGCACACGCTGTAAGTTGTTTTTGATGTCGGACAGCACACCCTCGGTGGTCTGCGCCACAGAGATACCGTCGTTGGCGTTCCGGGCCGCCTGAGTTAGGCCCTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCCGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCCGAAGACAGACGCTCGATAGGACTCGACAGCGCAGAATGGGTCTTTTTGGTATTCTTTTGAGTGATCAGCGAGAGGCTGTTGGTAttcacgact                                                  >  1:212431/1‑293 (MQ=255)                                                                                                                                                                                                                                                 gTACCGGTAGTGGCCTGTACCGTCAGTTCCCGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTCCGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGACAGAAGACAGACGCCCGCTAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCCGAGAGAGGTTGTTGGTATTCATGACt                                                  >  1:358287/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gTAGTGGCCTGTACCGTCAGTTTCCGCCCACGTTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCTGTGGTTTGCGCAAAGGAGACACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACAGTTCGCAACCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCAAGAAGACAAGCGCCCGATAGAACTCGACAGCGTAGACTGGTTTTTGTTTATGTTATTTTGGGTGATTAGAGAGGGGATGTTGGTAGTCATGGCTTGTGcc                                            >  2:396531/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTCATGTTAGAGGTGAAACGGTTAGCAACCGCATGACCCGCTGCGTCATCCTTCGCGTTGTTAATACGCAAGCCAGAAGACAGACGCTCGATCGAACTCGACCGCGCAGACTGGTTCTTTTTGATATTATTTTTAGTGCTCAGCGAGAGGCTGTTGGTATTAATGACTtgtgtc                                            >  2:143167/1‑298 (MQ=255)                                                                                                                                                                                                                                                        tAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCa                                           <  1:465128/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ggCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCNAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGAt                                       >  1:525425/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGtt                                  >  2:55947/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   aCCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTAt                                >  2:735325/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        cAGTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCtat                           <  1:693881/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          gTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCCACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTatat                         >  2:240532/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGTAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTatat                         <  2:573849/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          gTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTatat                         >  1:472604/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTCACGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTatat                         >  2:443154/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               cGCACACGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCaa                    <  1:418233/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cacaCGCTGTAGGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGt                  >  1:258941/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cacaCGCGGTAAGTTTTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGATGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGt                  <  2:245900/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     cGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTCTTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCCAGTCGtt              >  1:719431/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     cGCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGAAAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGtt              >  2:124961/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGTTg             >  2:200244/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       cTGTAAGTTGTTGTTGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCGAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCNGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGTTGa            >  2:543473/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gttgttgttGATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGTTGATTaagt      <  1:203813/300‑4 (MQ=255)                                                                                                                                                                                                                                                                                                 ttgttgATGTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGTTGATTACGTATTg  >  1:452640/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                    TGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCA‑TTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGATAGAACTCGACAGCGCAGACTGGTTCTTGTTGATATTATTTTGAGTGATCAGCGAGAGGCTGTTGGTATTAATGACTTGTGCCATGATTCGTTATCCTATATTGCAAGTCGTTGATTACGTATTG  >  NC_000913/2003060‑2003646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑