Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,317,195	G→A	Q10* (CAG→TAG)	trpA ←	tryptophan synthase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,317,195	0	G	A	98.8%	300.3 / NA	85	Q10* (CAG→TAG)	trpA	tryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (38/46);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGA  >  NC_000913/1316899‑1317490                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         gTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGc                                                                                                                                                                                                                                                                                                       >  1:634122/1‑300 (MQ=255)       cacCAGCTTAGCATACATCAACAGGCCAATGTGAATGGTCCGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACag                                                                                                                                                                                                                                                                                                 <  2:636931/300‑1 (MQ=255)           aGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGAt                                                                                                                                                                                                                                                                                             >  1:567311/1‑300 (MQ=255)            gATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCATTCTTTGCGCTCCTTCAACTAGGCAAACAGAGAtt                                                                                                                                                                                                                                                                                            >  2:638757/1‑300 (MQ=255)                ggCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCANCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCACGGATCAACGAGCGTGACGANAGGACCGAATGCGCCTTCTTTGCGCTCCTTCAAATAGGCAAACAGAGATTTGTa                                                                                                                                                                                                                                                                                        >  2:695697/1‑300 (MQ=255)                ggCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGTGCTCCTTCAACTAGGAAAACAGAGATTCGTa                                                                                                                                                                                                                                                                                        >  2:122435/1‑300 (MQ=255)                              ggCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGTGTTCAATCAGAt                                                                                                                                                                                                                                                                          >  2:242680/1‑300 (MQ=255)                                                   gtTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAat                                                                                                                                                                                                                                                     <  1:600406/300‑1 (MQ=255)                                                                  gTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑aa                                                                                                                                                                                                                                     >  1:341459/1‑300 (MQ=255)                                                                  gTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCAACGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCAATCAGATTTCCAATAGCGCTTTCAAAATATCGTGCACAGTG‑aa                                                                                                                                                                                                                                     >  2:114788/1‑300 (MQ=255)                                                                   tGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAg                                                                                                                                                                                                                                    <  1:615731/300‑1 (MQ=255)                                                                     ccAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGAt                                                                                                                                                                                                                                  <  1:588711/300‑1 (MQ=255)                                                                      cAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCAATCCGCCAGTGGGTCGTAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATg                                                                                                                                                                                                                                 <  1:753939/300‑1 (MQ=255)                                                                                aaaCATTGTGCCTGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATcgc                                                                                                                                                                                                                       <  2:268009/300‑1 (MQ=255)                                                                                aaaCATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAANTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGANCGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATcgc                                                                                                                                                                                                                       >  2:602647/1‑300 (MQ=255)                                                                                       gtgCAGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGGATGCGCCTTCTTTGCGCTCCTTAAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAAATTTCCCCTAGTGCTTTCAAAATATCGTAAACGGTG‑AAGATGTCTTTATCGCCGCGAcc                                                                                                                                                                                                                >  2:245129/1‑300 (MQ=255)                                                                                             gAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAg                                                                                                                                                                                                          >  1:188299/1‑300 (MQ=255)                                                                                             gAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCATCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAg                                                                                                                                                                                                          >  2:213821/1‑300 (MQ=255)                                                                                              aGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAgg                                                                                                                                                                                                         >  1:295078/1‑300 (MQ=255)                                                                                               gTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGt                                                                                                                                                                                                        >  1:632300/1‑300 (MQ=255)                                                                                               gTCACACCTGCAGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGAACGGAAAGGt                                                                                                                                                                                                        >  1:632654/1‑300 (MQ=255)                                                                                                     ccTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGTAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGTTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAacca                                                                                                                                                                                                  <  2:289990/300‑1 (MQ=255)                                                                                                     ccTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAacca                                                                                                                                                                                                  <  1:325227/300‑1 (MQ=255)                                                                                                       tGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAaccacc                                                                                                                                                                                                <  1:2260/300‑1 (MQ=255)                                                                                                         ccGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAg                                                                                                                                                                                              >  1:743311/1‑300 (MQ=255)                                                                                                           gCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTATGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTa                                                                                                                                                                                            <  2:145188/300‑1 (MQ=255)                                                                                                            cAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTNCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTANCGCCGCGACCGGAAAGGTTAACCACCAGTAg                                                                                                                                                                                           <  2:527929/300‑1 (MQ=255)                                                                                                             aaaGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATANTTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAgc                                                                                                                                                                                          <  1:521037/300‑1 (MQ=255)                                                                                                                ggCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAgctgc                                                                                                                                                                                       <  1:285507/300‑1 (MQ=255)                                                                                                                        gagTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATc                                                                                                                                                                               <  1:105585/300‑1 (MQ=255)                                                                                                                            ggCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGTGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCggg                                                                                                                                                                           <  2:46415/300‑1 (MQ=255)                                                                                                                               gTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGttt                                                                                                                                                                        >  2:727515/1‑300 (MQ=255)                                                                                                                               gTTTTGAATCGTCGGGCCATCCGCAAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCAACAGTAGCTGCTCTTTATCCGGGttt                                                                                                                                                                        >  2:367462/1‑300 (MQ=255)                                                                                                                                                    cGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTCTCGCCGCGACCGGAAAGGTTAACCAACAGCAGCTGGTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCa                                                                                                                                                   >  2:52375/1‑300 (MQ=255)                                                                                                                                                    cGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCa                                                                                                                                                   >  2:212254/1‑300 (MQ=255)                                                                                                                                                          tGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCGATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGTTG‑AAGATGTCTTTATCGCCGCGACCGGAAGGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGcc                                                                                                                                             <  2:160752/300‑1 (MQ=255)                                                                                                                                                             gTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAgg                                                                                                                                          >  1:294107/1‑300 (MQ=255)                                                                                                                                                               cggggaAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTTACGAAAGGAACGAATGCGCCTTCTTTGCGCGCCTTCAACTAGGCAAACAGAGATTCGTAGAGTTCCATCAGATTTCCCCTCGTGCGTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGGTAACCACCATTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGc                                                                                                                                        <  2:257772/296‑1 (MQ=255)                                                                                                                                                               cGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGNAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGCGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGc                                                                                                                                        <  2:649953/300‑1 (MQ=255)                                                                                                                                                               cGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGTCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGc                                                                                                                                        <  1:566385/300‑1 (MQ=255)                                                                                                                                                               cGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGc                                                                                                                                        <  1:321687/300‑1 (MQ=255)                                                                                                                                                                 gagaAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGt                                                                                                                                      >  1:646585/1‑300 (MQ=255)                                                                                                                                                                    aaGGGGATACCTAACTCCAGCGCGTCAGCACCTGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTggg                                                                                                                                   <  1:27942/300‑1 (MQ=255)                                                                                                                                                                      ggggATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACNGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGggag                                                                                                                                 >  2:659769/1‑300 (MQ=255)                                                                                                                                                                      ggggATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGggag                                                                                                                                 >  1:174267/1‑300 (MQ=255)                                                                                                                                                                      ggggATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATNTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGggag                                                                                                                                 <  2:557334/300‑1 (MQ=255)                                                                                                                                                                      ggggATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCATATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAATGAATGCGCCTTCTCTGCGCTCCTTCCACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCACCCTATCGTGCACGGTG‑AAGATGTCTGTATCGCCGCGACAGGAAAAGTTAACCACTAAGAGCTGCTCTTTCTCCGGGTTTCCGCGCATCATTTTCAAAGCATGGGcaatggcgcggaag                                                                                                                                 >  2:20633/1‑287 (MQ=255)                                                                                                                                                                         gATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGAt                                                                                                                              <  1:633074/300‑1 (MQ=255)                                                                                                                                                                            aCCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCAGATCACCGAGCGTGAAGAAAGGAACGAATGCGCCTTCTTTGTGCTCCTTCAAATAGGCAAACAGAGATTCGTCGCGTTTCACCAGATTTCCCCTCGTGCTTTCAAAATATCATGAACGGTG‑AAGATGTCTTTATCGCCGCGAACGGAAAGGTTAACCACCAGTCGCTGCTCTTTATCCTGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAAGATTcc                                                                                                                           >  2:241888/1‑300 (MQ=255)                                                                                                                                                                                 aCTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc                                                                                                                      >  1:432531/1‑300 (MQ=255)                                                                                                                                                                                 aCTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACAAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc                                                                                                                      <  2:479127/300‑1 (MQ=255)                                                                                                                                                                                             cAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATccc                                                                                                          <  1:557937/300‑1 (MQ=255)                                                                                                                                                                                                  ccGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGt                                                                                                     <  2:725281/300‑1 (MQ=255)                                                                                                                                                                                                        tCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGc                                                                                               <  1:445407/300‑1 (MQ=255)                                                                                                                                                                                                         cAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGca                                                                                              >  1:173342/1‑300 (MQ=255)                                                                                                                                                                                                                      gATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCCTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAg                                                                                 <  2:634113/300‑1 (MQ=255)                                                                                                                                                                                                                       aTAATTTTCAATGACTGCTCAATGTCCGGATCACCGAGCGTTACTAAATGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGTGCAAACAGAGATGCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGTTG‑AAGATGTCTTTATCGCTGCGACCGTAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATTGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAgg                                                                                <  2:394324/300‑1 (MQ=255)                                                                                                                                                                                                                                aaTGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCNCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAggg                                                                       <  1:501647/300‑1 (MQ=255)                                                                                                                                                                                                                                aaTGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCTGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAggg                                                                       <  1:713751/300‑1 (MQ=255)                                                                                                                                                                                                                                aaTGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAggg                                                                       <  1:452173/300‑1 (MQ=255)                                                                                                                                                                                                                                 aTGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGc                                                                      <  1:444855/300‑1 (MQ=255)                                                                                                                                                                                                                                  tGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCt                                                                     >  1:303111/1‑300 (MQ=255)                                                                                                                                                                                                                                     cTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtca                                                                  >  1:142459/1‑300 (MQ=255)                                                                                                                                                                                                                                            aTGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCGTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCgg                                                           <  2:634937/300‑1 (MQ=255)                                                                                                                                                                                                                                            aTGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCACTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCAACAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCgg                                                           >  2:362149/1‑300 (MQ=255)                                                                                                                                                                                                                                                  ggATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTAGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAg                                                     >  2:715926/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGa                                                    <  1:398117/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aTCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGac                                                   <  1:191321/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aTCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGac                                                   <  2:223796/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCACCTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCACCGGGATGATCCATTCGTGCAGGCACAGCGTTTTGCAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATc                                           >  1:312998/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          aCGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCTGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCTGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGc                             <  1:740125/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          aCGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGc                             <  1:41398/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          aCGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTCGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGc                             <  2:468225/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          aCGAATGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACG‑TGTAAGATGTCTTTATCGCCGCGACCTTAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGTGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGTTAATAGACACGTAATCAGCGCGTCCAGTGc                             <  1:1402/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               tGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCTGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTa                        <  1:561016/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               tGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTa                        <  1:462782/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               tGCGCCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGAACGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTa                        >  2:85249/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cgcCTTNTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCANATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAg                      <  2:614325/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cgcCTTCTTTGCGCTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAg                      <  1:334392/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gcTCCTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGtgttg          <  1:736428/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ccTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTgg       >  2:305449/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                ccTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTgg       >  1:12773/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                ccTTCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGAACGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGAATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTAATCATAGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTACGATACGCGTGTTGTgg       >  2:357097/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  ttCAACTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGc     <  1:122955/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     aaCTAGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGa  >  1:286665/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         GTTAAACACCAGATTGGCATACATCAACAGGCCAATGGGAATGGTCGGGTGTTTCTGGCGAATCAGTGCCAGCATTTCAAAACATTGTGCCGGAGTCACACCTGCCGCAAAGGCGCGCAGAGTGGCGTTTTGAATCGTCGGGCCATCCGCCAGTGGGTCGGAGAAGGGGATACCTAACTCCAGCGCGTCAGCACCGGCTTCAATTAGCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTG‑AAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGA  >  NC_000913/1316899‑1317490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑