Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	102,168	T→C	I468I (ATT→ATC)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	102,168	0	T	C	100.0%	304.6 / NA	85	I468I (ATT→ATC)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/49);  total (36/49)

AACCTGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCT  >  NC_000913/101884‑102459                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                    aaCCTGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGt                                                                                                                                                                                                                                                                                      >  2:107069/1‑300 (MQ=255)     tGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTCTCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCtg                                                                                                                                                                                                                                                                                  >  2:678944/1‑299 (MQ=255)     tGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCtt                                                                                                                                                                                                                                                                                  >  2:731534/1‑300 (MQ=255)     tGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCtt                                                                                                                                                                                                                                                                                  >  1:288219/1‑300 (MQ=255)     tGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCACGTTCtt                                                                                                                                                                                                                                                                                  >  1:288398/1‑300 (MQ=255)         aaTGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGTTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGc                                                                                                                                                                                                                                                                              <  2:443213/300‑1 (MQ=255)          aTGCTGTTTCAGCTGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATCCTTTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAACCGTTCGCTGTGTCGCACAGTTCGTGGACGTGGGAAAATTGATCCAATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGAACTGATTCTCGTTCCGGGGGCTGGTAATATCGGAAAAATTGCCCGTTTTTTAGCt                                                                                                                                                                                                                                                                             >  2:103371/1‑300 (MQ=255)               gTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAAt                                                                                                                                                                                                                                                                        >  2:88889/1‑300 (MQ=255)               gTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAAt                                                                                                                                                                                                                                                                        >  1:444191/1‑300 (MQ=255)                             gTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGc                                                                                                                                                                                                                                                          >  2:216871/1‑300 (MQ=255)                             gTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGc                                                                                                                                                                                                                                                          >  1:75275/1‑300 (MQ=255)                                             cgACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGgaaga                                                                                                                                                                                                                                          >  2:669428/1‑300 (MQ=255)                                               aCCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGaac                                                                                                                                                                                                                                        >  2:709416/1‑300 (MQ=255)                                                 cTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGaacaa                                                                                                                                                                                                                                      <  2:567500/300‑1 (MQ=255)                                                  tGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGaacaac                                                                                                                                                                                                                                     <  1:383280/300‑1 (MQ=255)                                                   gTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCACGTTCTTTAGCTGAAATCAAACTAAAGCCGCAACCTACGGAGGAGGCacaaca                                                                                                                                                                                                                                    >  2:270515/1‑300 (MQ=255)                                                             ttCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCGGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATaa                                                                                                                                                                                                                          >  2:668864/1‑300 (MQ=255)                                                                        gCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTcc                                                                                                                                                                                                               <  1:715681/300‑1 (MQ=255)                                                                             cGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCTANATGCTGGCACCGGTATTAACCGGTAANGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTtg                                                                                                                                                                                                          <  2:494166/300‑1 (MQ=255)                                                                                 ggTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGGTTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTTTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGCGGAGGAAAAACATGACTGATAAAGTCGCGGTATTGTtgggcg                                                                                                                                                                                                      >  2:236835/1‑298 (MQ=255)                                                                                 ggTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTtgggcg                                                                                                                                                                                                      >  1:426766/1‑298 (MQ=255)                                                                                   ttGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTtgggcggg                                                                                                                                                                                                    >  1:456767/1‑300 (MQ=255)                                                                                   ttGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGGTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTtgggcggg                                                                                                                                                                                                    >  2:640034/1‑300 (MQ=255)                                                                                      aTACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGAct                                                                                                                                                                                                 >  1:626353/1‑299 (MQ=255)                                                                                                        aaGTGTATCCGGCTGGCGAAGCGCCAATTCCNGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATNCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGtt                                                                                                                                                                               >  2:525807/1‑300 (MQ=255)                                                                                                            gtATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTtctc                                                                                                                                                                           >  1:409592/1‑300 (MQ=255)                                                                                                                             cgcCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGtctgtctcttatacacatctcgt                                                                                                                                                          <  1:425257/300‑23 (MQ=255)                                                                                                                             cgcCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGtctgtctcttatacacatctcgc                                                                                                                                                          <  1:426262/300‑23 (MQ=255)                                                                                                                              gcCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCgg                                                                                                                                                         <  2:130662/300‑1 (MQ=255)                                                                                                                                   ttCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGTAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTa                                                                                                                                                    <  2:468584/300‑1 (MQ=255)                                                                                                                                   ttCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTa                                                                                                                                                    <  1:109581/300‑1 (MQ=255)                                                                                                                                      cGGGAGCTGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGTTGCCGGACCCTGCGCAGGTAGCCTAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGTAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCTGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGcc                                                                                                                                                 <  2:243507/300‑1 (MQ=255)                                                                                                                                      cGGGAGCGGACAGCCGTTCGCTTTGTCGCACAATTCGTGGACGTGTGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCNGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCNGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGcc                                                                                                                                                 <  2:587643/300‑1 (MQ=255)                                                                                                                                      cGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGcc                                                                                                                                                 <  1:739469/300‑1 (MQ=255)                                                                                                                                      cGGGAGCGGACAGCCGTTCGCTGTGGCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCTAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAgga                                                                                                                                                 <  1:147749/300‑3 (MQ=255)                                                                                                                                          aGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCNGAAAAATTGCCCGTTCTTTAGCGGAANTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCgggc                                                                                                                                             <  2:488737/300‑3 (MQ=255)                                                                                                                                          aGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCACAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCgggc                                                                                                                                             <  1:267929/300‑3 (MQ=255)                                                                                                                                          aGCGGACAGCCGTTCGCTGCGTCGCACAATTTGTGGACGTTGGAAAATTGATCCCATTCTGGTGCCGGACCCGGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCTGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCgggc                                                                                                                                             <  2:250556/300‑3 (MQ=255)                                                                                                                                            cGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTg                                                                                                                                           <  1:733725/300‑1 (MQ=255)                                                                                                                                                    cGTTCGCTGTTTCGCACAATTCGTGGACGTGGGAAAATGGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCTGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAgg                                                                                                                                   <  2:30903/300‑1 (MQ=255)                                                                                                                                                       tCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCgg                                                                                                                                >  1:712133/1‑300 (MQ=255)                                                                                                                                                       tCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCgg                                                                                                                                >  2:247736/1‑300 (MQ=255)                                                                                                                                                           tgtgTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTAtt                                                                                                                            <  2:11697/300‑1 (MQ=255)                                                                                                                                                            gtgtCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTg                                                                                                                           >  2:478244/1‑300 (MQ=255)                                                                                                                                                            gtgtCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTg                                                                                                                           <  1:314797/300‑1 (MQ=255)                                                                                                                                                              gtCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATCGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTACGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGat                                                                                                                         >  2:48575/1‑299 (MQ=255)                                                                                                                                                               tCGCACAATTCGTGGACTTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGNGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTNCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGatg                                                                                                                        <  2:600683/300‑3 (MQ=255)                                                                                                                                                               tCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTNCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTNTTAGCCGGGCTGCGTGAAGGCGGTATTGatg                                                                                                                        <  2:526556/300‑3 (MQ=255)                                                                                                                                                               tCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGatg                                                                                                                        <  1:150508/300‑3 (MQ=255)                                                                                                                                                                 gCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGAtgcg                                                                                                                      <  1:653884/300‑1 (MQ=255)                                                                                                                                                                      atgcGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCTAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGNAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCNGGCTGCGTGAAGGCGGTATTGATGCGTATcc                                                                                                                 <  2:533435/297‑1 (MQ=255)                                                                                                                                                                      agttgtgtaCGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCTGGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCATTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGTAGTAAGAACAACATGACTGATAAAATCGCGTTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATcc                                                                                                                 <  2:150823/292‑1 (MQ=255)                                                                                                                                                                      aTTCGTGGACTTGGGAAAATTGATCCCATTCTGGTGCCGGACTCTGCGCAGGTAGCCGAGGTGCCGGCACCAGTAGCAACCGGTAACGACCTGATTCTCGGTCAGGGGGCTGGTAATATCGGAAAAAGTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCACAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGTGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATcc                                                                                                                 <  2:150444/300‑1 (MQ=255)                                                                                                                                                                      aTTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCTAGATGCTGGCACTGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGGAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCGGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGGGAAGGCGGTATTGATGCGTATcc                                                                                                                 <  2:150296/300‑1 (MQ=255)                                                                                                                                                                             taCGTGNGAAAATTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGANGCTTGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGAc                                                                                                          <  2:609971/299‑1 (MQ=255)                                                                                                                                                                                     aaaaTTGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAaaga                                                                                                  <  1:712960/300‑1 (MQ=255)                                                                                                                                                                                         ttGATCCCATTCTGGTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTc                                                                                              <  2:441172/300‑1 (MQ=255)                                                                                                                                                                                                       gTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAGTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGCATTCTGGCGTAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGAACCGAAAGAAGTCGACGTGACGCAACt                                                                                >  2:254504/1‑300 (MQ=255)                                                                                                                                                                                                       gTGCCGGACCCTGCGCAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACt                                                                                >  1:396732/1‑300 (MQ=255)                                                                                                                                                                                                           cggaccctgcgcAGGTAGCCTAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAg                                                                            <  1:730527/292‑1 (MQ=255)                                                                                                                                                                                                                 ctgcgcAGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATg                                                                      >  1:92918/3‑300 (MQ=255)                                                                                                                                                                                                                          tAGCCTAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGCTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAg                                                             <  2:448730/300‑1 (MQ=255)                                                                                                                                                                                                                          tAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAg                                                             <  1:450519/300‑1 (MQ=255)                                                                                                                                                                                                                                       ggCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCACGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCAGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGAACCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATcg                                                >  1:206168/1‑300 (MQ=255)                                                                                                                                                                                                                                              gTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTaca                                         >  1:387378/1‑300 (MQ=255)                                                                                                                                                                                                                                               tATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAACTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTacac                                        <  2:464823/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGt                                     <  1:218030/300‑1 (MQ=255)                                                                                                                                                                                                                                                     ccGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTGTCCTGTCGACCCGAAAGAAGTCGACGTNACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTcgc                                  >  2:549524/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ggTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCgcgg                                <  2:726509/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gACCTGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGAAGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGa                          >  1:662068/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGNGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGAt                      <  1:538222/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGAt                      <  2:731910/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGATTCTCGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGAt                      <  1:675098/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cGTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGGTGGTACGc               >  2:40536/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gTTCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCt              >  2:306442/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tCAGGGGGCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGc            <  1:248836/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             aGGGGGCTGGTAATATCGTAAAAATTGCCCGTTCTTTAGCTGAAATCAGACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGNACTTCCGCTGAGCTGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGNAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAg          <  2:596590/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gggCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGTAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAgggc       <  2:286163/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                gggCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAgggc       <  2:285987/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                gggCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAgggc       <  1:94992/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                gggCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACAAGGTCGCGGCGGTGAAGATGGTACGCTGCAgggc       <  1:249340/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                gggCTGGTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGCTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAgggc       >  2:285007/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                     gggaaTATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCTTAGGAAGAACAACATGACTGAGAAAATCGCGGTCCGGTTGTGCGGGACTTCCGCTGAGCTGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTTTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGTTGAAGATGGTACGCTGCAGGGCATGCt  <  2:35444/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ggTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACCTGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGCATGCt  <  2:36439/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ggTAATATCGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTAAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGCGGGACTTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGGCTGCGTGAAGGCGGTATTGATGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGTTTTCAGAAAGTGTTTATCGCGCTCCACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGCATGCt  >  1:230696/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                    AACCTGGTAATGCTGTTTCAGCCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATTCTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCT  >  NC_000913/101884‑102459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑